It has been shown that powerful Saussurea involucrata spirit has effects of expelling wind and removing dampness, anti-inflammation and alleviating pain, and activating blood flow and diminishing swelling. The pharmacokinetic findings show that this drug has advantages of slow-absorption, long-action and high-bioavailability.

Objective To screen the HBV SPⅡ promoter DNA-binding protein, and to investigate its potential role in the regulation of replication and expression of HBV DNA. Methods By using HBV SPⅡ biotinylated promoter DNA as a selective molecule, the T7 select human liver cDNA library was biopanned and the positive clones were selected. After screening, amplification of positive plaques was performed for inserted DNA fragment and then they were cloned into the pGEM-Teasy vector. Results Four positive plaques were chosen for DNA sequencing. The binding protein of HBV SPⅡ promoter was demonstrated as nicotinamide adenine dinucleotide (NADH) dehydrogenase 4 by BLAST. Conclusion The result suggests that this approach may provide a new tool for the study of replication and expression mechanism of HBV DNA.

Objective To investigate the association between TLR4,C-Jun N-terminal kinase,tumor necrosis factor-α(TNF-α),Leptin changes and chronic intermittent hypoxia(CIH). Methods Twenty-four SD rats were divided into 3 groups (n=8):control group,CIH 6 weeks group, CIH 8 weeks group. After the modeling,the change of blood pressure and renal artery were observed by the use of non-invasive blood pressure analyzer and HE staining. ELISA was employed to determine the production of TNF-αand Leptin in sera of these animals. The expressions of TLR4 and JNK mRNA were detected by real time quantitativer everse transcription-polymerase chain reaction ( QRT-PCR) method in the adipose tissue. Results The blood pressure in intermittent hypoxia group was signifi-cantly higher than those in control group(P<0.01), and the renal artery pathological changes in intermittent hy-poxia group were significantly heavier than those in control group;the content of TNF-α and Leptin in intermittent hypoxia was significantly higher than those in control group(P<0.01),and the content of TNF-α and Leptin was significantly increased with the days of hypoxia;the expression of TLR4 and JNK at mRNA levels in intermittent hy-poxia was significantly higher than those in control group(P<0.01),and the expression of TLR4 and JNK at mR-NA levels was significantly increased with the days of hypoxia. Conclusion TLR4,JNK,TNF-α,Leptin may be in-volved in the inflammatory response of chronic intermittent hypoxia in rats.

Objective To study the effect of the extract of total flavonoids of Chrysanthemum indicum(TFC) on adjuvant arthritis(AA) synoviocytes.Methods Totally 0.1ml of the complete Freund's adjuvant was subcutaneously injected into the right hind feet pads of 20 SD rats.24 days after immunity synoviocytes in the knee joint were treated with TFC.Cell morphology was examined with electron microscopy.Protein level of caspase-3 cleaved fragments was analyzed by Western blotting.The annexin V stain assay was applied to explore the effect of caspase-3 inhibitor on the amelioration in synovial cells apoptosis of AA rats.Results Typical morphology and biochemical feature of apoptosis in synovial cells of AA rats were observed with TFC.The protein level of caspase-3 cleaved fragments increased obviously and was related with the concentration of TFC in synovial cells of AA rats.The apoptotic cells positively stained with annexin V were markedly reduced by caspase-3 inhibitor.Conclusion TFC can induce apoptosis in AA rats synoviocytes,which may achieve therapeutical effects in AA.The activation of caspase-3 may be one of the main causations.

Brain injury is one of the most serious diseases in neonatal period,which can cause cerebral palsy,motor development delay,cognitive dysfunction and learning difficulties and other sequelae,and severely affects the health development and quality of life of the newborn.Neonatal brain injury (NBI) is a wide range of diseases caused by a variety of causes,its clinical manifestations lack specificity,clinically,it is difficult to judge the severity,duration and the time of prenatal injury,and it has been paid much attention to by scientific researchers and clinicians.At present,imaging method is a major means of NBI diagnosis,but imaging examination is usually a lag and limitations.Levels of humoral biomarkers change early after brain injury,and early brain injury can be predicted by detecting their changes.In recent years,a variety of sensitive brain damage biomarkers have been detected in various body fluids of newborns,mainly including neuron-specific enolase (NSE),ubiquitin carboxyl hydrolase L1 (UCH L1),S100B protein,tau protein,myelin basic protein (MBP),glial fibrillary acidic protein (GFAP) and activin A and so on.the application and research progress of these commonly used biomarkers in NBI are reviewed in this paper.

Objective To investigate the effect of escitalopram treatment on cognitive bias to the emotional facial information in patients with panic disorder. Methods 30 patients met CCMD-3 criteria for panic disorder were enrolled as research group and marched sexual and age 30 healthy persons enrolled as control group. Patients were treated with escitalopram for 8 weeks. All participants measured with dot-probe task of emotional facial information at base and after 8 weeks. RTs and attentional bias scores were compared respectively. Results After 8 weeks,HAMA scores (7. 81 ± 2. 52) in research group were lower than that of at base ( 17. 23 ± 3. 12) (P = 0.002). A repeated measure ANOVA revealed a significant probe site main effect (F(1,58) =4. 34, P = 0.031 ) , RTs of antarafacial site were longer than that of homonymy site. It revealed a significant probe site and group interaction(F(1,58) =16.15, P=0.000) ,a significant emotional facial information type and probe site interaction(F(1,58)=9.25, P =0.015) ,and a significant emotional facial information type × probe site× group interaction(F(1,58) =7. 31, P = 0. 002). LSD test showed that RTs of antarafacial site to fear facial information in research group were longer than that of homonymy site(P = 0.0009). RTs and attention bias scores of antarafacial site to fear facial information after 8 weeks in research group were lower than that of at base(P=0.032,0.008). Conclusion Patients with panic disorder have the cognitive bias to the fear facial emotional stimulus, and escitalopram treatment might improve the cognitive bias.

Objective:To optimize the extraction process for the total saponins from the root of Thladiantha dubia Bunge. Meth-ods:The extraction technology was optimized by orthogonal experiment with the dissolution content of total saponins as the index and the extraction times, extraction duration and ratio of solid to liquid as the influencing factors. Results:The optimum extraction condi-tions for the total saponins from the root of Thladiantha dubia Bunge were as follows:the reflux extraction was conducted twice(1. 5 h per time) with 70% ethanol as the solvent, and the ratio of solid to liquid was 1 ∶6. Conclusion:The optimum extraction technology is simple, reproducible and stable.

Objective To examine the effects of p38 mitogen-activated protein kinase (MAPK) inhibitor on the behavioral response to zebrafish larvae after hypoxia/reoxygenation brain injury and to identify whether the protective effect is mediated by inhibiting apoptosis and protein,and mRNA related to apoptosis.Methods The 5-day post-fertilization zebrafish larvae were randomly assigned to 3 groups:control group,model group and intervention group.Fishes in the intervention group were separated into 3 subgroups according to p38 MAPK inhibitor concentration (5,10,20 μmol/L).The activity levels of the larvae were analyzed by using quantization mode of ZebraLab software,swimming distance and moving speed were recorded.Terminal transferase dUTP nick end labeling (TUNEL) assays of brain assays were performed.The protein levels of phosphorylation of p38 MAPK,apoptosis related proteins of B-cell lymphoma-2 (Bcl-2),Bcl-2 associated X protein(Bax) and Caspase-3 were determined by Western blot.The mRNA expressions of Bcl-2,Bax,and caspase-3 were also analyzed by reverse transcription-quantitative PCR (RT-qPCR).Results The activity movement analysis of the intervention group 2 and 3 demonstrated a significantly increase in the swimming distance compared with the model group(P ＜ 0.05).After irradiation under strong light,all groups showed dramatically increasing in the moving speed.After removal of strong light,a significant decrease in moving speed was found in the control group and intervention group 2 and intervention group 3.The TUNEL assay showed that apoptosis index decreased in the intervention group (21.7 ±2.0,12.8 ± 1.9,17.7 ±2.6) compared with model group (46.8 ±5.3) (all P ＜0.01).Western blot assays demonstrated a significant increase protein level of phosphorylation of p38 MAPK after hypoxia and reoxygenation,and the inhibitor reduced the p-p38 MAPK expression.Compared with the model group,p38 MAPK inhibitor increased the protein and mRNA expression level of Bcl-2,whereas reduced the Bax and caspase-3 expression in the brain.Conclusions Under the influences of p38 MAPK inhibitor,zebrafish larvae improved the behavioral changes after hypoxia-induced brain injury.The inhibitor (10 μmol/L) optimally reduces hypoxia-induced apoptosis in brain by up-regulating Bcl-2,down-regulating Bax/caspase-3 protein and their mRNA level.

Objective To investigate the association between mycoplasma infection and pathogenesis of renal cell carcinoma(RCC). Methods There were 95 samples of archived embedded tissues of RCC and 60 samples of archived embedded normal renal tissues around the tumor for comparison.We detected mycoplasma hyorhinis P40 expression in archived embedded renal tissues by immunohistochemistry(IHC) with mycoplasma hyorhinis monoclonal antibody(MAb) PD4. Results Mycoplasma hyorhinis P40 was present in 64.2%(61/95 cases) of RCC and in 21.7%(13/60 cases) of the paratumor normal renal tissues in controls.The mycoplasma hyorhinis P40 expression ratio in RCC was significantly higher than that in the normal renal tissue,around the tumor(P

Objective To investigate the association between mycoplasma DNA and renal cell carcinoma (RCC). Methods There were 95 samples of archived embedded tissues of RCC. We detected mycoplasma DNA in the tissues by nested polymerase chain reaction (PCR) with mycoplasma universal primer. Results The mycoplasma DNA detection ratio in RCC samples was 81.1%. The samples with moderate and low differentiation had a higher mycoplasma DNA ratio (90.9%) compared with that of high differentiation samples (72.6%) (P