The effect of high dos; dexamethasone(5mg/kg wt, intravenous injection)to prevent and treat the secondary pathological damage of craniocerebral injury was studied in animal models of craniocerebral injury caused by high-velocity missiles in dogs.We observed ths physiological changes, analyzed the value of serum and. cerebrospinal fluid lactate dehydrogenase, estimated the permeability of blood brain barrier (BBB) and studied brain pathology by light and electron microscopy.The results suggest high dose dexamethasone can help to restore ths structure and function of BBB, protect brain cells as well as lessai the secondary pathological damage in tha respiratory and circulatory systems and reduce th production of lipoptrox-ides(LPO).

In order to provide an experimental evidence for clinical utility, we undertook a series of experimental studies of glucocorticosteroid to treat closed head injury and gun shot brain wound, For studies of closed injury in rats, there were four groups as follows: Group 1: methyl prednisone 30mg/kg (MPG); Group 2: dexamethasone 5 mg/kg (HDMG): Group 3: dexamethasone0.5mg/kg (LDMG); and Group 4: normal saline as a control group (CG) For study of gun shot wound of brain, The dogs were divided into three groups, Grup 1 (wound group, n=8); Group 2 (treated group, n=7) and group 3 (control group, n=7). After injury, the vital signs were observed, the water content of the brain and Even' s blue level were measured and the brain were examined under optical and electronic microscopy, All results proved that secondary traumatic edema in the animals used high dose glucocotricosteroid were much more slight

The left ventricular bands of isolated human heart were studied by microscopy, echocardi ography and gross anatomical method. The results indicate that the structures of the bands were those commonly observed in human heart. They could be classified into two types: myo-bands and tendinous bands. However, we could only differentiate the “bands whose” diameter was more than 1.5mm with Aloka-SSD720 echocardiograph (made in Japan). we suggest that the resolution of the instrument we used was too low, and this might be the main reason that the detection of left ventricular bands was much lower than the incidence, we classified these bands according to the adhering points and diameters of bands.In this paper, the forming factors of bands and their clinical significance are also discussed.

As a new way,on-line bid & purchase expands and deepens the bid principle of "Honest,Justice,Public". This paper analyzes the characteristics of on-line bid,puts forward the centralized bidding frame based on Internet,and optimizes the bid procedure. The study not only makes the on-line bid more effective and strict but also makes the bid result more convincing.

Objective To compare the effects of transabdominal hysterectomy(TAH),transvaginal hysterectomy(TVH),and laparoscopic assisted vaginal hysterectomy(LAVH).Methods Clinical data of 48 cases of TAH(TAH Group),38 cases of TVH(TVH Group),and 31 cases of LAVH(LAVH Group) were retrospectively analyzed.Results The operation time was shorter in the TAH Group(73.7?5.9 min) than in the TVH Group(80.9?7.0 min) and the LAVH Group(129.3?9.1 min)(F=612.04,P=0.000).The intraoperative blood loss was greater in the LAVH Group(142.8?17.1 ml) than in the TAH Group(128.1?9.6 ml) and the TVH Group(129.7?10.2 ml)(F=15.18,P=0.000).The postoperative analgesic requirement rate was higher in the TAH Group(75.4%,36 cases) than in the TVH Group(30.2%,11 cases) and the LAVH Group(38.4%,12 cases)(?~2=20.310,P=0.000).The length of postoperative hospitalization was longer in the TAH Group(7.3?1.6 d) than in the TVH Group(4.8?1.0 d) and the LAVH Group(5.1?1.1 d)(F=47.07,P=0.000).The postoperative pyrexia rates were not significantly different among the TAH Group(8.4%,4 cases),the TVH Group(7.4%,3 cases),and the LAVH Group(8.2%,3 cases)((?~2=0.074,) P=0.964).Conclusions As compared with transabdominal hysterectomy,transvaginal hysterectomy and laparoscopic assisted vaginal hysterectomy show advantages of less invasion,less pain,and quicker recovery.

Objective To explore whetherthere exists Schwann-cell derived neurotrophic factor (SDNF) receptor in peripheral nerve. Methods SDNF binding sites in rat sciatic nerve were studied using 125 Ⅰ-SDNF as a radioligand (radioligand binding assays). Results There exists SDNF specific binding sites in peripheral nerve,and the specific binding sites have the following characteristics:①The equilibrium dissociation constant (kd) was (93.11?0.52) pmol/L.②The maximal binding capacity (B max ) of SDNF was (8.91?0.26)fmol/mg protein.③Saturation.④Kinetic studies revealed that the association rate constant (K 1) was (3.91?0.63)?10 7M -1 ?min -1 and the dissociation constant (K -1 ) was (3.38?0.54)?10 -3 min -1 .⑤Specific studies of SDNF binding sites showed that the binding sites were highly specific for SDNF. Conclusion SDNF receptor exists in the peripheral nerve and the peripheral nerve may be one of target tissues of SDNF.

Observations on the developement of pathological changes of rat brain, together with dynamic detection of CN- concentrations in blood and brain tissue and quantitive analysis of brain cytochrome oxidase activity, are carried out within 24 h after acute cyanide intoxication (4.5mg/kg i.p.) . The results indicate that in the cyanide poisoning with the dose under lethality (80%LD) , the pathological changes in rat brain appear, especially in cytochrome oxidase poorly- contained areas, including! 1 ) degeneration and necrosis of neurons and gliocytes; 2) degeneration, swelling and lysis of different cell projection components; 3) the myelinoclasis of myelinated nerve fibers. Those changes undergo a dynamic course divided into three phases: 1 ) the phase of metabolic disturbance; 2) of response to injury; and 3) of restoration. The authors consider that the acute poisoning displayed by the animal after NACN injection is directly caused by the intense inhibation of brain cytochrome oxidase; the secondary lesions of brain structure may be responsible for the manifestations such as trembling, unstable, and ataxia etc.occur later. The mechanisms of the brain pathological changes after cyanide intoxication are also disscussed.

The changes of immunoreactive TRH (TRH-ir) in cerebrospinal fluid (CSF) and plasma before and after acute traumatic head injury were determined with radioimmunoassay (RIA) on a feline model of acute experimental head trauma. The results showed that the concentrations of TRH-ir in experimental animals pre- and 2, 4, and 6h post-injury were 119.48?51.77, 460.71 ?178.72, 377.27? 139.33, and 280.17?110.46pmol/L in CSF, and 122.58?28.87,1158.89?163.18, 909.69?160.55, and 545.38?132.80pmol/L in plasma, respectively, while those in control animals measured at the corresponding time were 112.22?53.42, 105.36?49.64, 100.63?52.89, and 104.52?52.42pmol/L in CSF, and 113.57 ?25.79, 107.32?26.60, 119.84?31.53, and 117.21?28.95pmol/L in plasma, respectively. The contents of TRH-ir in CSF and plasma in the experimental group after traumatic head injury were significantly higher than those, in the control group (P

The effects of high frequency jet ventilation(HFJV) on brain resuscitation had been investigated in 28 cats in which simple brain functional failure was produced by occlusion of both carotid and both vertebral arteries. The results suggested that HFJV could improve endurance of cats when subjected to brain ischemia, raise the survival rate of cats with reversible brain functional failure, and prolong the survival time of cats with permernent brain functional failure. The mechanisms of how HFJ would exert beneficial effect on brain resuscitation was discussed.

Through expounding the connotation of clinical physicians'humanistic medicine practice ability,this paper pointed out that building the evaluation index system should abide by the four principles of systematicness,scientificity,guidance and feasibility.Constructing the evaluation index system of clinical physicians' humanistic medicine practice ability included 4 first-level indicators,13 second-level indicators and 47 third-level indicators,which is beneficial to promoting the physicians' humanistic medicine practice ability and is conducive to improve the human resource evaluation and management of hospitals.