Aim To study the inhibitory effect of Oxymatrine-Baicailin compound on the secretion of hepatitis B viral antigens in HepG 2.2.2.15 cells.Methods HepG2.2.2.15 cells were cultured and treated with a series of Oxymatrine,Baicailin,or Oxymatrine-Baicailin compound respectively.The toxicity effect was determined by MTT colorimetry.Con-tents of the hepatitis surface antigen(HBsAg) and hepatitis e antigen(HBeAg) in the culture supernatants were determined by Enzyme linked immunosorbent assay.Results Oxymatrine at concentrations between 0.125 and 1 g?L~(-1) had little toxicity effect on cells,but Oxymatrine at 2 g?L~(-1) and 4 g?L~(-1) had much more toxicity effect on cells.The inhibitory effect of Oxymatrine on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1).The toxicity of Baicailin on cells increased from 0.625 to 2 g?L~(-1),especially when the dose surpassed 1 g?L~(-1).The inhibitory effect of Baicailin on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1),but its efficacy was inferior to Oxymatrine's efficacy.Oxymatrine-Baicailin compound had good inhibitory effect on hepatitis B viral antigens secretion,and the inhibition effect of the compound on HBeAg was superior to the effect on HBsAg.The Group C Oxymatrine-Baicailin compound had good synergism inhibition effect on hepatitis B viral antigens secretion and the inhibition rate of the specific group compound was significantly superior to that of Oxymatrine treatment alone(HBsAg:P=0.043;HBeAg: P=0.026).Conclusion Oxymatrine-Baicailin compound has good synergism effect on hepatitis B viral antigens secretion in HepG2.2.2.15 cells.

Animals ; Breast Neoplasms ; pathology ; Cell Division ; physiology ; Cells, Cultured ; Humans ; Macrophages ; cytology ; Signal Transduction ; immunology ; physiology ; Space Flight ; Tumor Cells, Cultured ; Weightlessness

Objective To investigate the efficacy and safety of tranexamic acid in perioperative blood loss in old patients with multi-level lumbar spinal stenosis.Methods From January 2009 to September 2010,a total of 68 consecutive patients with multi-level lumbar spinal stenosis ( ≥65 years old) underwent posterior decompression,internal fixation and bone graft fusion who were randomly divided into group A and group B with 34 patients in each.The patients in group A received tranexamic acid and the patients in group B received an equal volume of normal saline.The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion,the number of the patients needing blood transfusion and hemoglobin,fibrinogen,prothrombin time and so on were examined preoperatively and 24 hours postoperatively.All the patients were observed for the clinical symptoms of deep vein thrombosis.Results The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion and the number of the patients needing blood transfusion in group A were significantly decreased than those in group B[ (641.1 ± 128.4) ml vs.(780.1 ± 107.3) ml,(228.80 ± 52.07) ml vs.(345.50 ±42.16) ml,(1.02 + 1.56) U vs.(2.89 ± 1.76) U,16 cases vs.28 cases ],there were significant differences between two groups (P ＜ 0.05 ).As for the value of postoperative hemoglobin concentration in group A [ ( 104.00 ± 4.87) g/L ] was significantly higher than that in group B [ (92.20 + 5.47 ) g/L ] (P ＜ 0.05 ).There were no significant differences in the levels of fibrinogen,prothrombin time,and activated partial thromboplastin time between two groups (P ＞ 0.05).No deep vein thrombosis was found 7 days postoperatively.Conclusion Tranexamic acid can be effectively used in spine surgery in old patients with multi-level lumbar spinal stenosis without increasing the risk of venous thrombosis.

ObjectiveTo study the relation of phonation and respiration in normal subjects. MethodsUsing Aerophone Ⅱ Model 6800 and Visipitch 6097, vital capacity was recorded first , then the simultaneous phonation volume, maximum sustain phonation time,mean airflow rate,sound pressure level and average pitch were recorded in order to induce the relation of phonation and respiration in normal subjects. ResultsThere was a linear correlation between phonation volume and vital capacity. Maximum sustain phonation time varied with changes of mean airflow rate and phonation volume and mean airflow rate varied little in different subjects and had no relation with average pitch and sound intensity. ConclusionMaximum phonation time, the mean airflow rate and ratio of phonation volume to vital capacity can be used as indicator in clinical application.

Objective To investigate the effect of goal-directed fluid treatment (GDT) on prognosis during emergency operation for craniocerebral trauma patients.Methods Thirty craniocerebral trauma patients treated with emergency craniotomy from January 2015 to October 2015 were divided into control group (n =15) and GDT group (n =15),according to the random number table.In control group,the patients [ten males and five females,age of (40.3 ± 12.1) years and weighting (62.1 ± 9.1) kg] were given conventional fluid therapy based on the intraoperative mean arterial pressure,heart rate,central venous pressure,etc.In GDT group,the patients [eleven males and four females,age of (44.5 ± 9.6) years and weighting (64.0 ± 6.9) kg] received GDT based on the stroke volume variation (SVV) under Vigileo monitor.Serum levels of neuronspecific enolase (NSE) and S100-β were respectively detected by electrochemical luminescence and ELISA method before anesthesia induction (T1),1 h after dura incision(T2),immediately after surgery (T3),6 h after operation(T4),24 h after operation(T5) and 48 h after operation (T6).Intracranial pressure and cerebral perfusion pressure (CPP) were detected at all time points,and Glasgow Coma Score (GCS) at T1,T5 and T6.Postoperative infection,brain edema,hospital stay in ICU,total hospital stay and death rate were recorded.Results Compared with control group,levels of NSE in GDT group were decreased at T4,T5 and T6 and levels of S100-β in GDT group were decreased at T3,T4,T5 and T6 (all P ＜ 0.05).Intracranial pressure in GDT group was not significantly different from that in control group (P ＞ 0.05).GCS at T6 was higher in GDT group than that in control group (P ＜ 0.05).Length of ICU stay and length of total hospital stay in GDI group were (4.5 ± 2.1) d and (14.3 ± 3.6) d respectively,shorter than (6.3 ± 1.9) d and (18.3 ± 4.0) d in control group (P ＜ 0.05).None experienced infection,brain edema and death after operation.Conclusion Compared with conventional fluid therapy,GDT is beneficial to the prognosis of craniocerebral trauma patients undergoing emergency craniotomy without increasing intracranial pressure and brain edema,and GDT can reduce hospital stay.

Objective To study the expressive levels of KAI-1mRNA,Kiss-1mRNA and MTA1 in the benign and malignant lesions of breast,and their clinicopathological significance.Methods We detected the expression levels of KAI-1mRNA,Kiss-1mRNA and MTA1 in the sections from 60 cases of breast cancer and 30 cases of benign lesions of breast.Results The positive rates and scores of KAI-1mRNA and Kiss-1mRNA were significantly lower in breast cancer than those in benign lesions(P

The activity of the negative regulator of cell cycle, P27, affects the normal process of cell cycle. As a type of cell cycle diseases, tumor is closely correlated with the activation of P27. It is shown that the level of P27 decreases in most kinds of tumors.By its influence on cell proliferation and apoptosis, P27 plays an important role in the occurrence and development of tumor. The activity of P27 is regulated by several pathways either at or after transcription, so various ideals which directly change the protein level of P27 or indirectly interfere the activities of elements in regulative pathways of P27 will provide many novel and efficient ways for therapies in tumors.

Casein was hydrolysed with sulphuric acid under pressure and the hydrolysate was oxidized and nitrated by a mixture of sulphuric acid and potassium nitrate. After decolorization and neutralization to pH 4, the residue was removed by filtration.After adjusting the filtrate to pH 6.8 and supplementing it with tryp-tophane, cystine, methionine, and tyrosine, it was used as the source of amino acids ill the preparation of the medium for the microbiological assay of phenyl-aianine with Lactobacillus ardbinosus 17-5 as test microorganism.The standard curve shows that the medium is almost free from active phenyl-alanine, for blank values are of the same magnitude as those of a synthetic medium.Increasing the quantity of nitrated casein hydrolysate from 8 g. to 10 g. doesnot cause any difference in acid production. Further supplementation of themedium with fustidine, arginine, and lysine is also without effect.The results obtained by analyses of several foods with the present medium and the findings of Sauberlich and Baumann (1946), and Schweigert (1944) are in

BACKGROUND:Maxil ary sinus floor elevation via a lateral window approach is the most effective method of overcoming the shortage of bone mass deficiency in atrophic maxil ary posterior region. Bone transplantation is considered to be a prerequisite for the success of maxil ary sinus floor elevation. Platelet-rich plasma and platelet-rich fibrin have been used to accelerate bone formation, regeneration, and repair. However, few in-depth studies are reported on the effects of concentrated growth factors on new bone formation. OBJECTIVE:To investigate the effects of concentrated growth factors applied in maxil ary sinus floor elevation via a lateral window approach with simultaneous implant placement on repair of bone defects and new bone formation. METHODS:This was a randomized double-blind control ed trial, which was performed at the Hospital of Stomatology, Southwest Medical University, China. Forty patients presenting with loss of maxil ary molars and residual bone height of 2-5 mm in the posterior maxil ary region were included in this study. These patients were randomly assigned to either receive concentrated growth factors, hydroxyapatite and autogenous bone (experimental group, n=20) or hydroxyapatite and autogenous bone (control group, n=20) at bone defect sites. X-ray examination was performed 1, 3 and 6 months after surgery to evaluate bone regeneration and repair as per excel ent and good rate. The thickness of the cortical bone at the defect sites was measured using a dental cone beam CT scanner to reflect bone density. The study protocol had been approved by Ethics Committee of Hospital of Stomatology, Southwest Medical University, China, was performed in strict accordance with the Declaration of Helsinki, and had been registered with ClinicalTrial.gov (identifier NCT03046173). Written informed consent had been obtained from each patient prior to enrol ment. RESULTS AND CONCLUSION:This study was completed in 2016. Study outcomes were obtained as fol ows:X-ray images showed that 3 and 6 months after surgery, bone regeneration around the implant was better in the experimental group than in the control group (P<0.01) and better bone regeneration in the experimental group was observed at 6 months than at 3 months after surgery. At 1, 3 and 6 months after surgery, bone density at bone defect site was significantly greater in the experimental group than in the control group (P<0.01). These results suggest that concentrated growth factors applied in maxil ary sinus floor elevation via a lateral window approach with simultaneous implant placement can promote new bone formation and accelerate synosteosis.

This review is to provide an overview of current situation and advance of abdominal compartment syndrome. Progress has been made in diagnosis and therapy of abdominal compartment syndrome. At present patients who are diagnosed as abdominal compartment syndrome are associated with a high mortality rate. Therefove, it is important to diagnoze and treat the disease early. Surgical treatment of increased intraabdominal pressure leads in most instances to a rapid and profound correction of the physiological abnormalities. Operative treatment is the unique and effective approach of abdominal compartment syndrome.