Aim To study the inhibitory effect of Oxymatrine-Baicailin compound on the secretion of hepatitis B viral antigens in HepG 2.2.2.15 cells.Methods HepG2.2.2.15 cells were cultured and treated with a series of Oxymatrine,Baicailin,or Oxymatrine-Baicailin compound respectively.The toxicity effect was determined by MTT colorimetry.Con-tents of the hepatitis surface antigen(HBsAg) and hepatitis e antigen(HBeAg) in the culture supernatants were determined by Enzyme linked immunosorbent assay.Results Oxymatrine at concentrations between 0.125 and 1 g?L~(-1) had little toxicity effect on cells,but Oxymatrine at 2 g?L~(-1) and 4 g?L~(-1) had much more toxicity effect on cells.The inhibitory effect of Oxymatrine on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1).The toxicity of Baicailin on cells increased from 0.625 to 2 g?L~(-1),especially when the dose surpassed 1 g?L~(-1).The inhibitory effect of Baicailin on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1),but its efficacy was inferior to Oxymatrine's efficacy.Oxymatrine-Baicailin compound had good inhibitory effect on hepatitis B viral antigens secretion,and the inhibition effect of the compound on HBeAg was superior to the effect on HBsAg.The Group C Oxymatrine-Baicailin compound had good synergism inhibition effect on hepatitis B viral antigens secretion and the inhibition rate of the specific group compound was significantly superior to that of Oxymatrine treatment alone(HBsAg:P=0.043;HBeAg: P=0.026).Conclusion Oxymatrine-Baicailin compound has good synergism effect on hepatitis B viral antigens secretion in HepG2.2.2.15 cells.

Animals ; Breast Neoplasms ; pathology ; Cell Division ; physiology ; Cells, Cultured ; Humans ; Macrophages ; cytology ; Signal Transduction ; immunology ; physiology ; Space Flight ; Tumor Cells, Cultured ; Weightlessness

Burns ; therapy ; Fluid Therapy ; Humans

Burns ; complications ; Humans ; Shock ; etiology ; prevention & control ; Universal Precautions

Burns ; complications ; therapy ; Fluid Therapy ; Humans

Burns ; complications ; metabolism ; therapy ; Fluid Therapy ; Humans ; Hypoxia ; etiology ; metabolism ; prevention & control ; Ischemia ; etiology ; metabolism ; prevention & control

This review is to provide an overview of current situation and advance of abdominal compartment syndrome. Progress has been made in diagnosis and therapy of abdominal compartment syndrome. At present patients who are diagnosed as abdominal compartment syndrome are associated with a high mortality rate. Therefove, it is important to diagnoze and treat the disease early. Surgical treatment of increased intraabdominal pressure leads in most instances to a rapid and profound correction of the physiological abnormalities. Operative treatment is the unique and effective approach of abdominal compartment syndrome.

ObjectiveTo study the relation of phonation and respiration in normal subjects. MethodsUsing Aerophone Ⅱ Model 6800 and Visipitch 6097, vital capacity was recorded first , then the simultaneous phonation volume, maximum sustain phonation time,mean airflow rate,sound pressure level and average pitch were recorded in order to induce the relation of phonation and respiration in normal subjects. ResultsThere was a linear correlation between phonation volume and vital capacity. Maximum sustain phonation time varied with changes of mean airflow rate and phonation volume and mean airflow rate varied little in different subjects and had no relation with average pitch and sound intensity. ConclusionMaximum phonation time, the mean airflow rate and ratio of phonation volume to vital capacity can be used as indicator in clinical application.

Objective To induce experimental autoimmune myasthenia gravis (EAMG) rabbits by using T?125～147 Methods Peptides T?125～147 were synthesized referring to the residue sequence of acetylcholine receptor of Torpedo California and the rabbits were inoculated with the peptides Clinical manifestation was graded in 4 levels Electrophysiological function was assessed by repetitive nerve stimulation (RNS) and single fibre electromyography (SFEMG) tests Anti peptides antibodies were evaluated by enzyme linked immunosorbent assay (ELISA) Student t test was used to analyze the difference between the EAMG and healthy rabbits Results Following the second inoculation,the rabbits appeared weakness Clinical symptoms were improved by neostigmine At 3,5 and 10 Hz,the decrement of compound muscle action potential (CMAP) and the mean jitter (MCD) of the immunized rabbits were higher than in the healthy ones The percentage of decrement of CMAP in order of the control group and the T?125～147 group were:3 Hz: 1 625?1 317,25 375?7 945; 5 Hz: 2 000?1 732,25 625?9 102; 10 Hz: 1 750?1 392,28 875?8 709.Following the above sequence,the MCD were:3 Hz: (9 875?1 126) ?s,(25 875?7 945) ?s; 5 Hz: (11 375?0 916) ?s,(27 500?3 381) ?s; 10 Hz: (12 375?1 061) ?s,(31 000?4 811) ?s Anti peptide antibodies in immunized groups were significantly higher than those in the control group Conclusion T?125～147 might be served as the immunogenic to induce EAMG in rabbits,accompanied by elevation of anti peptide antibodies and the blockage of neuromuscular transmission

BACKGROUND:Maxil ary sinus floor elevation via a lateral window approach is the most effective method of overcoming the shortage of bone mass deficiency in atrophic maxil ary posterior region. Bone transplantation is considered to be a prerequisite for the success of maxil ary sinus floor elevation. Platelet-rich plasma and platelet-rich fibrin have been used to accelerate bone formation, regeneration, and repair. However, few in-depth studies are reported on the effects of concentrated growth factors on new bone formation. OBJECTIVE:To investigate the effects of concentrated growth factors applied in maxil ary sinus floor elevation via a lateral window approach with simultaneous implant placement on repair of bone defects and new bone formation. METHODS:This was a randomized double-blind control ed trial, which was performed at the Hospital of Stomatology, Southwest Medical University, China. Forty patients presenting with loss of maxil ary molars and residual bone height of 2-5 mm in the posterior maxil ary region were included in this study. These patients were randomly assigned to either receive concentrated growth factors, hydroxyapatite and autogenous bone (experimental group, n=20) or hydroxyapatite and autogenous bone (control group, n=20) at bone defect sites. X-ray examination was performed 1, 3 and 6 months after surgery to evaluate bone regeneration and repair as per excel ent and good rate. The thickness of the cortical bone at the defect sites was measured using a dental cone beam CT scanner to reflect bone density. The study protocol had been approved by Ethics Committee of Hospital of Stomatology, Southwest Medical University, China, was performed in strict accordance with the Declaration of Helsinki, and had been registered with ClinicalTrial.gov (identifier NCT03046173). Written informed consent had been obtained from each patient prior to enrol ment. RESULTS AND CONCLUSION:This study was completed in 2016. Study outcomes were obtained as fol ows:X-ray images showed that 3 and 6 months after surgery, bone regeneration around the implant was better in the experimental group than in the control group (P<0.01) and better bone regeneration in the experimental group was observed at 6 months than at 3 months after surgery. At 1, 3 and 6 months after surgery, bone density at bone defect site was significantly greater in the experimental group than in the control group (P<0.01). These results suggest that concentrated growth factors applied in maxil ary sinus floor elevation via a lateral window approach with simultaneous implant placement can promote new bone formation and accelerate synosteosis.