Objective To investigate the effect of lansoprazole injection on 24-hour intragastric pH in healthy volunteers. Methods The volunteers inclouding 10 women and 10 men, were divided into experitmental group and control group randomly, each group of 10 cases. After testing the pH initial value in stomach through pH determination electrode, the cases in experitmental group were drip the drug of lansoprazole injection, the cases in control group were drip the same volume of 0.9% sodium chloride solution. Then constantly determined the value of pH in the stomach for 24 h. Results The pH value in the stomach of expertimental group after injected lansoprazole was significantly higher than that before administration (P < 0.05). The pH value in the stomach of expertimental group was higher than that in control group between finished of injection and 24 h, especially in the first 12 h after administration, there were significantly differences (P < 0.01). The pH value in experimental group after injected lansoprazole for 24 h was 4.565 ± 1.109, in control group was 2.211 ± 0.263, there was significant difference (P < 0.01). The percentage of maintenance time of pH reaching 4.0 and 6.0 in stomach in experimental group were (73.36 ± 21.08)% and (53.22 ± 23.76)% , in control group were (13.28 ± 10.55)%and (3.92 ± 2.17)%, there were significant differences (P<0.01). Conclusions The strength of acid suppresion of lansoprazole injection is worthy of approving, and it can work at full capacity in treatment of patients with peptic ulcer complicated with upper gastrointestinal hemorrhage if lansoprazole is given every 12 h.

Objective To investigate the efficacy of wax therapy plus functional training in treating ankylosing spondylitis. Method One hundred patients were randomly allocated to treatment and control groups, 50 cases each. The treatment group received wax therapy plus functional training and the control group, functional training alone. One course of treatment consisted of seven days in both groups. The therapeutic effects clinical were evaluated after two course of treatment. Result Joint pain severity and morning stiffness time improved in both groups after treatment (P＜0.05 or P＜0.01) and did more in the treatment group than in the control group (P＜0.01). The total efficacy rates in the two groups were 84.0%and 66.0%, respectively;there was a statistically significant difference between the two groups (P＜0.05). Conclusion Wax therapy plus functional training is safe, reliable, convenient and easy to perform in clinical treatment of ankylosing spondylitis.

Objective To evaluate the distribution of Pneumoconiosis and provide scientific basis for preventing the occur-rence of Pneumoconiosis and improving the life quality of patients for the workers in coal mine of Songzao.Methods Retrospective epidemiological research and chi-square test were used for all collected Pneumoconiosis cases in Songzao coal mine of Chongqing from 2010 to 2012.Results From 2010 to 2012,the incidence was significantly different(χ2 =25.048,P <0.05),and the prevalence was significantly different(χ2 = 11.223,P <0.05).Besides,the mean duration of dust exposure was(20.50± 5.45)years.Mean-while,the difference of proportion between stageⅠand stageⅡ、stage Ⅲ show statistical significance(χ2 =9.162,P <0.05).What′s more,the rate of complicated pulmonary tuberculosis with pneumoconiosis was 2.94%.From 1979 to 2012,the difference in propor-tion of different stage′s fatality was significantly different(χ2 = 174.101,P <0.05).Conclusion Nearly three years,although the incidence and prevalence were all decreasing,but the situation was not optimistic.Pneumoconiosis are mainly in stageⅠ,and the le-thality of Ⅲ period is far stronger than the other two.Prevention of complication should be paid high attention.

Objective Multiple organ failure (MOF) has been regarded as a continuous, uncontrolled inflammatory response. Intestinal mucosal mast cells (IMMC) may be involved in MOF. Substance P (SP), one of gut peptides, is an important regulator in the neuro-endocrine-immune network. However, the effects of SP on IMMC, especially in the case of MOF, remain unclear. This study was aimed to investigate the effects of SP on IMMC in the development of MOF. Methods The rat model of MOF was established by injecting zymosan. After thirty minutes of the injection, SP was given through tail veins at the dose of 20 nmol/kg weight and 0.2 nmol/kg weight. The concentrations of histamine and tumor necrosis facfor-?(TNF-?) in plasma and intestine tissues were measured. The pathological alterations of essential organs including intestine, liver, kidney and lung were examined under light microscope. Their corresponding functions were reflected with ALT, Cr and PO 2. The ultrastructure of the IMMC was also observed under a transmission electronic microscope. Results Compared with the controlled rats, those injected with SP showed much more serious inflammatory response under light microscope. Both ALT and Cr increased by about 50%, but PO 2 decreased by about 40%. Histamine level in the intestinal tissue of the rats treated with SP remarkably decreased, whereas the plasma histamine level did not show any significant changes. The level of TNF-? was higher in the intestinal tissue of the rats treated with SP but no change in plasma, and the degranulation of IMMC under transmission electronic microscope was more obvious.Conclusions SP may trigger MOF through acting on IMMC which may release inflammatory mediators such as histamine and TNF-?.

Objective To investigate the effect of somatostatin(SST) on the activity of intestinal mucosal mast cells(IMMC) and its pathological significance in the development of multiple organ failure(MOF). Methods The rat model of MOF was established by the peritoneal injection of zymosan. Thirty minutes after the injection of zymosan, SST at 2.300 ng?kg -1 ?h -1 or 0.023 ng?kg -1 ?h -1 was injected respectively through tail veins. The concentration of histamine and tumor necrosis factor-? (TNF-?) in plasma and intestinal tissue were measured. The pathological alterations of essential organ including intestine, liver, kidney, lung and heart were studied under light microscope. Their corresponding functions were reflected with alanine aminotransferase (ALT), cretinine (Cr) and oxygen pressure (PO 2). In addition, the ultra structure of the IMMC was observed under a transmission electronic microscope. Results Compared with the controlled rats, the rats injected with SST (2.300 ng?kg -1 ?h -1 ) showed less serious inflammatory response under light microscope. ALT and Cr were decreased 53% and 60% respectively. However, the lung ventilation was improved and PO 2 was increased by 50%. The histamine level in the intestinal tissue from rats treated with SST remarkably increased( ( 8.60? 0.50 ) ng/g protein to ( 14.50? 1.08 ) ng/g protein), whilst the plasma histamine level did not show any significant changes. Exogeneous SST also resulted in lower level of TNF-? in intestine but no changes in plasma. Furthermore, degranulation of IMMC from the rats treated with SST was less obvious. Conclusion SST may prevent from or arrest the development of MOF through suppression of the release of inflammatory mediators, such as histamine and TNF-?.

OBJECTIVE To know the situation and problems of anti-infective agents used in clinics in our hospital. METHODS Antibacterial use of 1771 cases discharged from hospital in April 2005 was retrospectively analyzed. RESULTS The use rate of anti-infective agents was 94.81%,in which the drug combination therapy was the majority and the rate was 55.63%,and the rate of the prophylaxis of infections was 63.61%;of the first 10 drugs the use rate of second line and better drugs was 36.21%;the use rate of anti-infective agents used for Ⅰ incision for more than 4 days was 90.30%;the use rate of anti-infective agents used in obstetrics was 98.17%. CONCLUSIONS We should enhance the supervising of the use of anti-infective drug and mush adopt management measure.

Thin basement membrane nephropathy(TBMN) is one of the most common disorders of the kidney,affecting at least 1% of the population.It seems to be a disease of the adult glomerular basement membrane(GBM) type Ⅳ collagen trimer ?3∶?4∶?5.Genetic evidence indicates that autosomal TBMN is caused by heterozygous mutations in either COL4A3 or COL4A4, whereas homozygous or combined heterozygous mutations in the same genes lead to autosomal recessive Alport syndrome.The author summarized the epidemiology,clinical features,renal biopsy,genetics,pathogenesis,diagnosis and therapy of TBMN.

Objective To study the difference of the peripheral blood lymphocyte gene expression in the glioblastoma patients and the normal subjects by the gene chip engineering technique. Methods The human gene expression spectrum chip containing 13 824 cDNA was used to detect the gene expression in the peripheral blood lymphocytes of 23 glioblastoma patients and 40 normal persons. The lymphocyte mRNA in the glioblastoma patients and healthy subjects was withdrawn. The cDNA probe with the different fluorescein was made by reverse transcription. The probe and the chip were hybridized. The fluorescence intensity was scanned and digitized,and gene expression was compared between the two groups. Results The peripheral blood lymphocytes in the glioblastoma patients had 96 genes to present the difference expression with that of normal subjects,including 25 upregulated genes and 71 downregulated genes. Conclusion The gene expression in the peripheral blood lymphocytes of glioblastoma patients and normal subjects exists difference. The lymphocyte DNA union,the abnormal function of transcription and signal conduction are the possible reasons for the different immune function between the glioblastoma patients and the healthy subjects.

Objective:To investigate the effects of substance P(SP),somatostain(SST) and vasoactive intestinal peptide(VIP) on the activation of rat intestinal mucosal mast cells(IMMC) in vitro.Methods:IMMC isolated and purified from the whole intestines of normal rats were incubated with gut peptides at various concentrations.The histamine concentration in IMMC and their supernate were determined.Furthermore,the ultrastructure of the incubated IMMC was observed under a transmission electronic microscope.Results:①The spontaneous release rate of histamine was (22.86?3.22)%.②SP significantly increased the histamine release rate from IMMC(P0.05)④At the concentration from 1?10 -1 mol/L to 1?10 -8 mol/L,the higher concentration of VIP was used,the lower histamine release rate was observed(P

Dendritic Cells ; Humans ; Rhinitis, Allergic, Perennial ; Rhinitis, Allergic, Seasonal