Animals ; Depression ; etiology ; metabolism ; Frontal Lobe ; metabolism ; Glutamic Acid ; metabolism ; Male ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley ; Serotonin ; metabolism ; Stress, Physiological ; physiology ; Swimming

Cardiovascular Diseases ; Follistatin-Related Proteins ; physiology ; Humans

Abortion, Induced ; adverse effects ; Acupuncture Therapy ; Adult ; Female ; Humans ; Infertility, Female ; etiology ; therapy ; Pregnancy

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Arrhythmias, Cardiac ; diagnosis ; genetics ; therapy ; Child ; Child, Preschool ; Electrocardiography ; Humans ; Infant ; Male ; Middle Aged ; Syndrome

Chromatography ; methods ; DNA Fingerprinting ; Drugs, Chinese Herbal ; analysis ; Magnetic Resonance Spectroscopy ; Mass Spectrometry

Competing endogenous RNA (ceRNA) is a class of RNA which includes mRNA,pseudogenes,long non-coding RNA (lncRNA),circular RNA (circRNA).ceRNA weakens its inhibitory effect on mRNA translation through competitive binding with shared microRNA (miRNA).Many studies have confirmed that the disorder of ceRNA is closely related to the occur-ence of breast cancer,gastric cancer,lymphoma and other tumors.With the improvement of researches,ceRNA may be used as a tumor marker of clinical diagnosis and therapeutic target.

Functional constipation(FC) in childhood is characterized by a low defecation frequency in combination with either involuntary loss of stools(encopresis),passage of large amounts of stool,retentive posturing,or hard and often painful defecation.It represents 3%-5% of general pediatric outpatient visits and up to 25% of pediatric gastroenterology consultations.FC is a diagnosis made by history and physical examination.No testing is necessary or desirable.The decision to seek medical care for symptoms arises from a parent′s or caretaker′s concern for children.The caretaker′s threshold for concern varies with his or her experiences and expectations,coping style,and perception of illness,till now there is not an universal native criteria established in spite of much has been done in it.The criteria used are still to draw assistance from the foreign standard,among which the classic criteria,the Rome Ⅱ and Rome Ⅲ criteria are considered commonly used.The purpose of this paper is to descript the several foreign criteria,and to make comparison among them,and to explore their applicability and effectiveness in childhood FC

Animals ; Herpesviridae ; genetics ; metabolism ; Humans ; Viral Proteins ; chemistry ; genetics ; metabolism

Objective To explore the normal reference values of infant intelligence in Fagan test of infant Method Fagan test was carried out in 315 healthy infants aged 6.5～14.0 month in Shenyang,Tieling and Jinzhou in Liaoning province.Results Infants with cognitive exponent X≥54.6 might be considered to be normal in development,49.9

Objective To construct recombinant human Bcl-2 adenoviral vector. Methods Sense human Bcl-2 cDNA fragment from plasimid pcDNA3 1/bcl-2 was cloned into adenovirus shuttle vector pAdCMV. pAdCMV/bcl-2 and plasmid pJM17 were cotransfected into 293 cells with lipofectamine 2000. Replication defective adenovirus was reproduced and purified. Adenoviral virus DNA was detected with polymerase chain reaction(PCR), and plaque assay was performed to determine the titer of virus. Results Recombinant adenovirus containing bcl-2 target gene was obtained. The titer of virus was 2 0?10 10 pfu/ml. Conclusion High titer of adenovirus containing target gene was successfully obtained by recombinant adenovirus vector.