Objective]To discuss and analyze Professor Cheng Jing's clinical experience for treating decline in ovarian reserve(DOR). [Method]Through learning from the tutor in the outpatient service and case studies ,we analyzed and summarized Pro. Cheng's recognition on the etiology and pathogenesis of DOR,together with his dialectical way of thinking, combined therapy of Chinese and western medicine ,and empirical prescriptions. [Result] Pro. Cheng believes that this disease lies in the kidneys as wel as in the thoroughfare and conception meridian ,the pathogenesis is kidney deficiency and the imbalance of the thoroughfare and conception meridian,or combined with the stagnation of liver qi,spleen deficiency,and blood stasis and phlegm fire (heat); the therapy is mainly nourishing and warming kidneys as wel as conditioning the thoroughfare and conception meridian ,combined with Chinese medicine's periodical therapy,such as adjusting the circulation of qi and blood in livers,spleens,hearts and other organs. [Conclusions] Pro. Cheng's combined therapy of Chinese and western medicine for curing DOR ,of which his empirical prescriptions the Bushenyichongkangshuaitang is the main part,is of remarkable curative effect and significant clinical value.

Economy type medicine is the requirement of economy type society,the demand of the development of the hygiene sector,the necessary part of medical ethics education.The economy type medicine has concrete content.In the process of building harmonious and economy type society,we need to enhance the economy type ethics education,strengthen the economy type consciousness of medical personnel and medical student.

Fetal intrauterine growth restriction is one of the common diseases in the perinatal period,which not only increases the child morbidity and mortality in the perinatal period,affecting the long-term development of the nervous system,and can make the risk of adult obesity,hypertension,type Ⅱ diabetes increased significantly.A number of studies suggest that the down-regulation of A amino acid transporter expression is closely associated with intrauterine growth restriction.The down-regulation of A amino acid transporter expression may be an important cause of intrauterine growth restriction.

Objective To explore the variation about the application of fast-track surgery and laparoscopy in treatment for colorectal cancer in recent years.To investigate the probability of combining protocols of the two for treatment for colorectal cancer.Methods The clinical and basic literatures of related researches about colorectal treatment of laparoscopy and fast-track surgery were collected and reviewed.Results Compared with the traditional treatment modalities,both of fast-track surgery and laparoscopy used for the treatment of colorectal cancer have better clinical effects.Conclusions Fast-track surgery and laparoscopic techniques used for the treatment of colorectal cancer are feasible,but the combination of the two should be confirmed by further randomized controlled trials.

Objective To investigate the effects of Benazepril on inflammatory cytokines in patients with primary hypertension and its efficacy analysis.Methods 72 patients with mild to moderate primary hypertension were randomly divided into benazepril group(37 cases)and control group(35 cases).The control group patients were given felodipine sustained release tablets Stag,1 times daily.Benazepril group were given benazepril tablets 10mg,1 times daily.Both groups were treated for 8 weeks.Changes in blood pressure and heart rate,plasma inflammatory cytokine levels and the adverse reactions were observed.Results After 8 weeks of treatment,the patients' blood pressures were significantly decreased (t =2.87,2.43,2.31,2.23,P ＜ 0.05 or P ＜ 0.01) than before in both groups.And the decrease in the benazepril group was more obvious than that in the control group (t =2.21,2.13,all P ＜ 0.05),while the patients' heart rates in the two groups showed no significant change (P ＞ 0.05) ; Patients' plasma IL-4 and IL-8 levels decreased significantly,plasma IL-10 levels increased significantly (t =2.99,2.87,3.21,2.13,2.19,2.23,all P＜0.05 or P＜0.01),and the magnitude of decrease or increase in the benazepril group were more obvious compared with the control group (t =2.21,2.13,2.34,all P ＜ 0.05).Conclusion Benazepril in the treatment of primary hypertension has good antihypertensive effect and security which can significantly reduce the plasma levels of proinflammatory cytokines IL-4 and IL-8 levels,improve the anti-inflammatory cytokine IL-10 levels,and has the role of suppressing the inflammatory response of the vessel wall.

Gene therapy has emerged as an efficient modality to treat human diseases.This method is based on the transfer of genetic material to tissues to induce a curative effect.Gene therapy vectors are molecular constructs used to facilitate the penetration of genomic sequences inside the cells.Viral vectots have however several limitations when administered directly to the patient.They may cause significant toxicity by activating innate immunity or by eliciting an adaptive immune response against viral proteins.In addition,targeting the vector to the desired site is an issue when given systemically.The use of cells as vehicles for gene therapy vectors has many advantages.The combination of cell-viro-gene therapy has been thought as a new and promising strategy for therapy of cancer.The targeting vector to cancer stem cells will become a new direction in the field of gene therapy.In this article,we will introduce progressions,limitations and future directions of gene therapy of liver cancer.

Objective To elucidate the MRI appearance of prostatic abscess,the DWI and enhanced MRI features.Methods 12 cases of prostatic abscesses were retrospectively analyzed,the clinical symptom mainly manifested as lower urinary tract symptoms and fever.All of the patients were given routine MR examination including DWI sequence,6 patients received further enhanced MR examination.Results In the 12 cases,there were 4 cases behaved as single type,8 cases as multifocal type.The abscess showed iso-or slightly hypo-signal intensity on T1 WI,hyper-signal intensity on T2 WI,markedly high signal intensity on DWI and correspond-ing markedly low signal intensity on ADC.Complete abscess walls showed iso-or slightly hyper signal on T1 WI,hypo-signal inten-sity on T2 WI.The mature abscess walls were thin and smooth,which showed homogeneously ring enhanced in 4 cases.The imma-ture abscess walls showed uneven thickness and moderately enhanced in 2 cases.Septum in the abscess could be found in 4 cases, which showed similar enhancement to the abscess walls,while the abscess cavity showed non-enhanced.Abscesses involved the sur-rounding structures in 2 cases,the involved area showed obvious hyper-signal on T2 WI fat-suppression sequence.Conclusion DWI is the best sequence in the diagnosis of prostatic abscess,the markedly high signal intensity on DWI is the characteristic sign.The enhanced MRI showed the walls and septa clearly,the extent and involvement of adjacent structures.The multi-parametric MRI is a prominent procedure in the diagnosis of prostatic abcess.

Objective To explore the diagnostic value of diffusion weighted imaging (DWI) and CT perfusion imaging (CTPI) in diagnosis of liver fibrosis. Methods Fifty-seven hepatic fibrosis patients and 23 normal controls received DWI (b=500 s/mm2), ADC value of different fibrosis stages was measured, while 35 patients and all 23 normal controls received CTPI. The parameters of CTPI including blood flow (BF), blood volume (BV), mean transit time (MTT), hepatic arterial fraction (HAF) and permeability surface (PS) were measured. Analysis of variances was performed to compare the difference among the groups in both examinations. ROC curve was used to analyze the sensitivity and specificity of DWI and CTPI. Results The difference of ADC value between control group and S1 group was not significant, but between control group and S2, S3, S4 group and among group S2, group S3, group S4 was significant. In the parameters of CTPI, only the difference of HAF between control group and S3-S4 group was significant. Sensitivity and specificity of DWI and CTPI was 78.90%, 82.60% and 66.67%, 73.91%, respectively. Conclusion DWI is superior to CTPI for early diagnosis and degrading of liver fibrosis.

Rat calcineurin (CaN) A alpha isoform (Ppp3ca) cDNA recombinant adenovirus vector was constructed in order to explore the effect of CaN on the myocardium apoptosis induced by ischemia-reperfusion injury. Total RNA was isolated from the heart of the adult Wistar rat, and Ppp3ca CDS segment of approximate 1.59 kb size was amplified by reverse transcriptional PCR method. Ppp3ca cDNA segment was cloned into pMD18-T Simple vector for sequencing, and the right clone was named T-Ppp3ca. Ppp3ca cDNA segment obtained from T-Ppp3ca was ligated with pShuttle2-IRES-EGFP to construct a recombinant plasmid pShuttle2-Ppp3ca-IRES-EGFP. Ppp3ca-IRES-EGFP expression cassette containing CMV, Ppp3ca-IRES-EGFP and SV40 polyA DNA fragment (3.97 kb) obtained from pShuttle2-Ppp3ca-IRES-EGFP was connected with pAdeno-X backbone sequence to construct a recombinant plasmid pAdeno-Ppp3ca. After being identified by PCR and enzyme digestion, recombinant plasmid pAdeno-Ppp3ca was packaged in HEK293 cells. Supernatant of adenovirus from HEK293 cells was collected after a visible cytopathic effect (CPE) appeared. The DNA of the recombinant adenovirus was extracted with the standard method. The presence of the recombinant adenovirus was verified by PCR. The results showed that sequencing results verified that the PCR product of Ppp3ca gene was identical to GenBank. Agarose electrophoresis showed the bands of recombined plasmid pAdeno-Ppp3ca and the recombinant adenovirus identified by enzyme digestion and PCR were in the right range corresponding with expectation. It was concluded that the recombinant adenovirus carrying rat calcineurin A alpha (Ppp3ca) cDNA as well as a report gene-enhancer green fluorescent protein gene was successfully constructed in this experiment.
Adenoviridae/*genetics ; Calcineurin/*biosynthesis ; Calcineurin/genetics ; Cloning, Molecular ; DNA, Complementary/genetics ; Genetic Vectors/genetics ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/genetics ; Myocardial Reperfusion Injury/*genetics ; Myocardium/chemistry ; Rats, Wistar ; Recombination, Genetic/genetics

In view of the current conditions and reasons about the reform of medical and health system in China,the paper expounds the feasibility of authorized operation with state-owned assets of public hospital and the concrete operational pattern from the angle of hospital operational mechanism, which proposes a new idea about how to effectively solve the contradiction between the limited investment on state-owned health resources and the growing demands on medical health.