Objective To explore the clinical efficacy of tension-free vaginal tape obturator inside-outside(TVT-O)in the treatment of female stress urinary incontinence.Methods In this study,we enrolled the patients with stress urinary incontinence who were treated with TVT-O(n=72)or midodrine hydrochloride(n=72).The patient self-evaluation and the Results of pad test and urodynamic test 6 and 24 months after the treatment were compared.Results These 2 kinds of treatment significantly improved the symptoms in patients with stress urinary incontinence,and in patients treated with TVT-O the clinical efficacy 24 months after the treatment was better.Conclusion TVT-O is safe and effective in treating female stress urinary incontinence,and the long-term follow-up and the improvement of urodynamic indices show it is better than simple drug treatment.

AlM:To analyze and explore the clinical efficacy of strengthening lateral tarsal strip and advancing lower eyelid retractor for the treatment of degenerative entropion with eyelid relaxation
METHODS: Reviewed from January 2011 to January 2013, 21 cases (33 eyes) of degenerative entropion eyelid relaxation were treated with operation of strengthening lateral tarsal strip and advancing lower eyelid retractor ( fornix conjunctival incision ) , the follow-up was 12-24mo, curative effect was observed.
RESULTS: All the patients were cured completely, have no recurrence or overcorrection phenomenon. The cure rate of operation was 100%, there was no complication.
CONCLUSlON: Operation of strengthening lateral tarsal strip and advancing lower eyelid retractor is a safe, reliable, efficient operation to the treatment of degenerative entropion with mode of eyelid relaxation.

Sera were collccted from mice immunized separately with schizonts, merozoitcs or different parasite stages, or from mice which had been recovered from Plasmodium yoelii yoelii infection. The effect of protection of cach serum against P. y. infection was assayed by passivc transfer technique. It was shown that only sera collected from mice which either had been trcated with chloroquine or had been naturally recovcred from P. y. infections can transfer certain dcgree of resistance to normal mice. The cffectivc sera delayed the appearance and the peak of parasitemia, and prolonged the survival time of the challenged mice. After these sera were fractionatcd by 50%(NH4)2SO4 precipitation, the prccipitated fraction showed similar degree of protection as the non-fractionated sera. Chloroquine-treated serum was shown to be able to inhibit the invasion of new erythrocytes by marozoite in vitro. 35S-methionine-labelcd antigens were precipitated by protective or non-protective sera and the bands were compared on SDS-PAGE and autoradiogram. Since thc antigcn bands of 245, 210, 190, 156 and 130 KD can only bc precipitated by protective sera but not by non-protectivc sera, it is possible that the protection of chloroquine-treated serum is induced by the specific antibodies against the above antigens.

Objective:To explore the anti-fatigue effect of Tibet maca in mice. Methods: The mice were respectively given the powder or the alcohol extract of Tibet maca. The lactic acid concentration in blood, serum lactate dehydrogenase ( LDH) , the time of weight loading swimming and serum urea ammonia level after the exercise in the mice were detected, and the anti-fatigue effect of the powder and the alcohol extract of Tibet maca was compared. Results: After the 30-day feeding, the serum LDH activity of the mice taking the powder or alcohol extract of Tibet maca was obviously higher than that of the mice in the control group(P<0. 05), the time of weight loading swimming was significantly longer than that in the control group (P<0. 05), and the blood lactic acid concentration after the exercise was obviously lower than that in the control group (P<0. 05). Conclusion: Tibet maca can improve the time of weight loading swimming of mice, and reduce the level of serum urea ammonia after exercise and blood lactic acid concentration, sug-gesting the powder and alcohol extract of Tibet maca have obvious anti-fatigue effect.

Culture ; Drugs, Chinese Herbal ; Formularies as Topic ; Humans ; Language ; Medicine, Chinese Traditional ; Tablets ; Translations

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Neural Conduction ; physiology ; Paraplegia ; diagnosis ; physiopathology ; Sural Nerve ; physiopathology

Objective To investigate the role of p38 mitogen-activated protein kinase (MAPK) signal transduction pathway in lipopolysaccharide (LPS)-induced acute lung injury (ALI).Methods Sixty male SD rats weighing 180-230 g were randomly divided into 4 groups: control group (group C, n = 6), ALI group ( n = 24),p38MAPK specific inhibitor SB203580 + ALI group (group SB + ALI, n = 24), SB203580 group (group SB,n =6). LPS 5 mg/kg was injected intravenously via tail vein in group ALI and SB + ALI, while the equal volume of normal saline was given instead in group C. Group SB + ALI received iv injection of SB203580 10 mg/kg via tail vein 30 min before LPS administration. Group SB received injection of SB203580. The rats were sacrificed at 1, 3 and6 h agter LPS administration (T1-3) in group ALI and SB + ALI (8 rats at each time point) andat 1 h after administration in C and SB groups. The lungs were immediately removed for microscopic examination and determination of phosphorylated p38MAPK (p-p38MAPK) expression, the concentration of protein in bronchoalveolar lavage fluid (BALF) and apoptotic index (AI). Another 32 rats were selected and randomly divided into 2 groups for survival study: ALI group and SB + ALI group ( n = 16 each), and then they were treated as mentioned above and observed for 48 h. Results The concentration of protein in BALF, AI and p-p38MAPK expression were significantly increased in group ALI and SB + ALI compared with group C, while decreased in group SB + ALI compared with group ALI ( P ＜ 0. 05 ). LPS-induced pulmonary histological changes were significantly attenuated in group SB + ALI compared with group ALI. The survival rate was significantly decreased in group ALI compgred with group SB + ALI ( P ＜ 0.01 ). Conclusion p38 MAPK signal transduction pathway is involved in LPS-induced ALI, which may be related to the apoptosis in the cells in the lung.

AIM: To evaluate the clinical effect and the rotational stability of AcrySof Toric intraocular lens ( IOL ) implantation to correct preexisting corneal astigmatism in cataract surgery.
METHODS: Twenty-three patients ( 28 eyes ) were enrolled from the department of ophthalmology in the first Affiliated Hospital of Nanjing Medical University. All patients underwent similar phacoemulsification procedure combined with AcrySof Toric IOL implantation from June 2012 to December 2013. The uncorrected visual acuity ( UCVA ) , best corrected visual acuity ( BCVA ) , anticipated residual astigmatism, postoperative residual astigmatism, and Toric IOL axis were detected and measured.
RESULTS:Three months after operation, the UCVA of all eyes were 0. 75±0. 16 and the BCVA were 0. 84±0. 15, there was no significant difference between UCVA and BCVA ( t = 1. 036, P>0. 05 ). The anticipated residual astigmatism was ( 0. 28±0. 12 ) D. The actual residual astigmatism after 3mo of the operation was (0. 42±0. 17) D. There was no significant difference between anticipated and actual residual astigmatism (t=1. 259, P>0. 05). The mean axis rotation of Toric IOL was 3. 02o±1.56o (0o-7o) after 1d of operation and 3. 28o±1. 85o (0o-7o) after 3mo. Among all the eyes, 25 eyes ( 89%) rotated <5o, in 3 eyes (11%) rotated 5o-7o.
CONCLUSION: The AcrySof Toric IOL implantation shows good effectiveness, predictability and stability in correcting pre-existing astigmatism in cataract patients.

Objective To establish an in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons in order to found a basis for research concerning the injury of oxygen glucose deprivation and axons regeneration. MethodsThe hippocampal neurons isolated from rats 24 h after born and cultured for 7 d were exposed to D-hanks solution with nitrogen gas instead of the original culture medium for 0.5, 1.0 and 1.5 h respectively, and then continue to be cultured in the original culture medium with oxygen. LDH content in the culture media was measured at 1, 5, 24, 48 and 72 h after reoxygenation. The morphological changes of neuron and axon were observed with inverted phase contrast microscopy. ResultsAfter oxygen-glucose deprivation/reoxygenation injury, hippocampal neurons became darker, swollen, and were with shorten axons. With the elapse of time, the LDH content was increased. The survival rate of hippocampal neurons was higher and the change of axon length was more obvious in the group of oxygen-glucose deprivation for 0.5 h than in the other groups. ConclusionAn in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons is successfully established.

BACKGROUND: Tongxinluo acts on benefiting qi, promoting circulation in collateral, activating blood circulation and resolving stasis, which is well effective in treatment of ischemic cerebral vascular disease. Single-photon emission computed tomography (SPECT) provides reliable reference evidence objectively and sensitively for clinical evaluation of therapeutic effects.OBJECTIVE: SPECT and clinical neurofunctional defect score (NDS)were used to observe the therapeutic effect of tongxinluo capsule on cerebral infarction.DESIGN: Case analysis was designed.SETTING: Internal Department of Neurology of First Affiliated Hospital of Chongqing Medical University.PARTICIPANTS: Totally 22 cases of acute cerebral infarction were admitted in Internal Department of Neurology of First Affiliated Hospital of Chongqing Medical University from March 2002 and July 2003, in which 12 cases were male and 10 cases were female, aged varied from 40 to 78 years, sick duration in range from 3 hours to 3 days.METHODS: Voluntarily, 22 cases of acute cerebral infarction were randomized in tongxinluo capsule group (capsule group)(11 cases) and the control (11 cases). In the control, danshen tablet was administrated,4 tablet/time, 3 times/d. In capsule group, tongxinluo capsule group was administrated, 4 pills/time, 3 times/d. The drugs were administrated for 15 days in two groups. Before and after treatment, SPECT ROI technique was used to assay ROI average radioactive counts on the affected and healthy sides to understand local blood flowing volume of brain in two groups. Neurofunctional defect evaluation was done before and after treatment on each patient.in two groups.RESULTS: Totally 22 cases of cerebral infarction all entered result analcontrol after treatment [(49.182±5.344 5), (28.364±4.610 3) score P ＜ 0.001].the affected side of the control and treatment group before treatment was decreased. Fifteen days after treatment, the volume in original decreased flowing area of brain was improved after taking the capsule (66.536±18.676,ter administration of the capsule reflected in SPECT radionuclide, but it was not improved remarkably in the control.CONCLUSION: Tongxinluo capsule improves definitely cerebral blood flow in patients with cerebral infarction. With improvement of blood flowing in brain, the neurofunctional defect score is promoted clinically, too. It is explained that the capsule promotes the recovery of nerve function.