Objective To investigate the role of ECRG2 gene in migration and invasion of human fibrosarcoma HT1080 cells. Methods Tet-on system was used to set up ECRG2-inducible cell clones.Knockdown of ECRG2 gene was done by stably expressing short hairpin RNA (shRNA) specific for ECRG2 mRNA in a highly invasive fibrosarcoma cell line HT1080. The expressions of ECRG2 proteins were detected by Western blotting assay. Wound-healing scrape assay and transwell invasion assay were performed to assess the effect of ECRG2 gene on the invasive properties of HT1080 in vitro. Results The most effective ECRG2siRNA interference and inducible was selected by Western blotting assay. Knockdown of ECRG2 gene significantly promoted the migration and invasion of HT1080 cells while expression of ECRG2 impaired the migration and invasion of HT1080 cells in vitro. Conclusion ECRG2 gene is correlated with the invasive and metastatic potential of fibrosarcoma carcinoma, and expression of ECRG2 induces the inhibition of migration and invasion of HT1080 cell line.

Objective To evaluate the clinic effect of two kinds of interventional intracavitary closure combined with deligation of varicose veins of lower extremity. Methods Seventy-five sick legs of 68 cases,23 sick legs of 21 cases accepted interventional intracavitary closure combined with high deligation, while 52 sick legs of 47 cases accepted interventional catheter electric coagulation combined with high deligation. Results The average hospitalization time was(2.7±1.4)days, lyins in bed time was 6 hours,12 sick legs(16.0%)suffered superficial skin mild numbness. The follow-up time was(7.3±3.0)months, all the varicose veins were occluded by color Doppler and no local recurrence was found. Conclusion Interventional intracavitary closure is a kind of safe, convenient and effective therapy to varicose veins of lower extremity.

Objective To study the quality control method for Chanshukang granule. Methods TLC and HPLC method were used to identify the Chinese herbal medicines. The determination was carried on HPLC, using Kromasil C18 column (200 mm?4.6 mm, 5 ?m), ethanol-2% glacial acetic acid (40∶60) as mobile phase and detected at the wavelength of 324 nm. Results The Chinese herbal medicines, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Placenta Hominis and Radix Glycyrrhizae Preparata, in granules were identified by TLC. The calibration curve was linear in the range of 0.625～3.750 ?g for ferulic acid. The contents in three batches were 0.054%, 0.037%, 0.042% respectively. The average recovery was 96.2% and RSD=2.37%. Conclusion The TLC method can be used for the quality control of four herbal medicines in the granule. The HPLC method is simple, sensitive, accurate and provides a certain evidence for controlling the quality of Chanshukang Granule.

Objective We aimed to assess systematically the effect of evidence-based nursing in the prevention of postpartum hemorrhage in puerpera of our country.Methods Relative literature was searched by computer at home according to the inclusion and the exclusion criteria,which was analyzed by RevManS.2 software,and literature selection and repetition was used according to the Note Express software.Results A total of 22 studies were brought into the study,the statistical result was analyzed by fixed effect model and the result was OR=0.29,95％CI (0.22,0.39),P ＜ 0.01.The sensitive analysis was carried out by replacement of statistical models,the result was OR=0.31,95％CI (0.24,0.42),P ＜ 0.01,which indicated that the result was stable and reliable.Conclusions The results of this study show that the application of evidence-based nursing can significantly reduce the postpartum hemorrhage rate in China,which is worthy of application in clinic.

Esophageal Diseases ; Humans ; Medicine ; Tracheal Diseases

Child ; Humans ; Laryngostenosis ; diagnosis ; therapy ; Tracheal Stenosis ; diagnosis ; therapy

Objective To explore the construction and application effects of extended care form for children with epilepsy based on Omaha System.Methods Based on Omaha System,the form was established by referring medical records,literature review and three rounds of focus group discussion.From February to June,2016,48 children with epilepsy were selected as the observation group and received routine care as well as management using the form based on Omaha System;from August to December,2015,48 children with epilepsy were selected as the control group and received routine care and follow-up.The effects of intervention and scores of Family Management Measure were compared between two groups.Results Nursing issues in all domains for the observation group 3 months after intervention were lower than those during hospitalization except financial situation (P＜0.05);there was statistically significant difference in scores of knowledge-behavior-status of main nursing issues before and after intervention except cognition and mental health items (P＜0.05).Duration of hospitalization,expenditure,readmission rate,EEG results and scores of FAMM in the observation group were better than those in the control group (P＜0.05).Conclusion The construction and application of the form based on Omaha System can provide references for longterm management for children.

Objective To assess systematically the effect of problem-based learning (PBL) versus traditional methods in nursing students.Methods Relative literature was searched by computer at home and abroad.according to the inclusion and the exclusion criteria,which were analyzed by RevMan5.2 software,and literature selection and repetition were used according to the Note Express software.Results A total of 9 studies of randomized controlled or not randomized controlled trial were brought into the study by random effects model.A total of 1 187 were collected into the study,574 in the experimental group and 613 in the control group.According to subgroup analysis showed by measurement scale,problem-based learning (PBL) can significantly improve the critical thinking ability of nursing students.The results had high stability and reliability.Conclusions PBL teaching method can significantly improve the critical thinking ability of nursing students,and provide a better learning method for clinical teachers and students,which is worthy of further promotion.

Objective: This study was to observe the changes of IL-6 gene expression in acute repetitive hypoxia. Method: When the ratio of living cells was more than 95%, nerve cells cultured were passed through with mixed gases of 95% N_2+5%CO_2 for 3 min followed by another gases mixture of 95% O2+5% CO_2 for 10 min. After repeated the experiment as above, the alteration in the expression of IL-6 gene was measured using the RT-PCR method. The prod ucts of PCR were analyzed with computer Gel imaging and image analysis instrument. Result: After the first hypoxia IL-6 gene expression enhanced, and after the second hypoxia, it was still stronger than baseline although it declined slightly. There was no significant RNA disintegration. Conclusion:The IL-6 involves in the cellular defense in hypoxia adaptation.

Objective To analyze the problem existing in the process of health records informatization con-struction and explore solution on the basis of situation of health record informatization in Yanta district of Xi'an city. And to provide bases for acceleration of residents'health records informatization construction in Xi'an.Methods Send 10 investigators to study and investigate the hospital information management system of 10 full-time public universities in Yanta district.Results There were a total of 25 711 faculties in 10 full-time public universities and 19 809 facul-ties had established electronic files,the filing rate was 77.04% A.On the whole,it seemed that the teaching staff in the filing rate(78.91%)was higher than that of the retired workers filing rate(73.78%),the two groups had statisti-cally significant difference(χ2 =88.164,P <0.05)B.Conclusion Xi'an Yanta district university faculty of electron-ic health records has exceeded the national average level,but there are still many problems need to be improved.We should strengthen government investment,the establishment of the region's unified information management standards, increase publicity and education efforts,and actively create the region's resource information sharing platform.