1.CONSTRUCTION OF IMMORTALIZEDBONE MARROW MESENCHYMAL STEM CELLS
Modern Hospital 2015;(1):13-15
Objective To establish immortalized bone marrow mesenchymal stem cells (BMSCs) from SD rats in vitro for further research on the characteristics and clinical application of BMSCs.Methods By using L ipo-fectamine TM 2000 -mediated gene transfection, plasmid pCMVSV40T/PUR containing the simian virus 40 large T antigene gene (SV40Tag) was transfected into BMSCs.BMSCs were screened by puromycin and then cultured on an extended scale.Their cell morphology and growth conditions were observed.Growth curve of cells was graphed.The expression of SV40Tag in transfected cells was identified by enzyme digestion method for the detection of tumor forma-tion.Results The growth rate of the experimental group of BMSCs after the 7th generation was significantly higher than that of the control group.No tumorigenicity was found in BMSCs after the 7th generation.Conclusion In vitro, the immortalized BMSCs by pCMVSV40T/PUR can provide basis on a large scale for its application in clinic and sci-entific research.
2.Evolution of the thematic structure of oncology based on the co-word analysis
Journal of International Oncology 2015;(10):795-800
Objective To explore the changes of the internal structure and research topics of oncology from 2005 to 2014,and offer reference for the development of the related studies and research policies. Methods The articles published in 203 journals in the area of oncologyfrom 2005 to 2014 included by JCR were chosen.Firstly,we cleaned the author keywords using the TDA software.After that,we standardized and categorized the keywords according to the Medical Subject Headings (MeSH)manually.In the end,we builted the co-occurrence matrixes of high-frequency words in three stages:2005—2007,2008—2010 and 2011 —2014, and drew the evolution maps and strategic diagrams of each stage to analyze the changes of the internal structure and research topics of oncology.Results While the word frequence increasing rapidly,the main keywords reduced gradually from 2005 to 2014.The traditional treatments such as chemotherapy and radiotherapy,the diagnostic and the epidemiological investigation were the core subject of oncology.With the screening of neoplasms,the research on the cell physiological phenomena and the genetic phenomena gained vigorous expan-sion.Breast neoplasms,the topic whose deep exploration also led the development of other topics,has always been the hard nut as well as the hot topic of the oncology.During this period,researches on the screening of neo-plasms and the genetic phenomena were developed rapidly.Conclusion The content of oncology will be more thoroughly with some merge,division,development,declining and perishing of the internal themes occurred as well.The boundaries among the sub-structure are becoming more and more blurred and the screening of neo-plasms and the genetic phenomena has gradually become the dominant trend in general practices.
3.The Origin of Ethical Problems in Medical Genetics and Relevant Strategies
Chinese Medical Ethics 1994;0(05):-
In the paper,we will deeply research sorts of ethical problems in medical genetics and their origins through a view of the human dual evolutions,and bring forward the viewpoint that any ethical problem can be originated from the conflict between human biological evolution and cultural evolution.Based on fully reasoning the relation between ethical problems in medical genetics and human evolution,the authors put forward a series of corresponding strategies to solve the ethical problems in medical genetics.
4.Progress of Angelman syndrome
Clinical Medicine of China 2021;37(1):93-96
Angelman syndrome is a neurodevelopmental disorder characterized by developmental delay, intellectual disability, dyskinesia, speech impairment, pleasant expression, epilepsy and abnormal electroencephalogram.The early symptoms of this disease are not typical, and attention should be paid to identification.In this article, the recent advances in clinical, ubiquitin-protein ligase E3A gene, genetic characteristics, genetic counseling and the treatment strategies of Angelman syndrome will be reviewed.
5.Glycotechnology in China.
Chinese Journal of Biotechnology 2015;31(6):797-804
Glycotechnology is a new branch of biotechnology, emerged early 1990's. In this article, the international background of glycotechnology is briefly introduced and history of glycotechnology in China is reviewed.
Biotechnology
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China
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Glycomics
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history
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History, 20th Century
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History, 21st Century
7.Investigation on relevant factors of coronary in-stent restenosis in elder patients with coronary heart disease
Chinese Journal of Primary Medicine and Pharmacy 2010;17(22):3043-3044
Objective To evaluate the elderly coronary in-stent restenosis risk factors and the incidence of restenosis. Methods Coronary angiography in 143 cases of patients with coronary heart disease were randomly selected, analyzing the characteristics of coronary heart disease, and 89 patients of them were reviewed coronary angiography, restenosis was observed, and the restenosis factors including age, sex, hypertension, diabetes, hyperlipidemia and coronary lesions, stent properties were analyzed. Results Statistical multi-vessel disease in patients with coronary heart disease was 73.9% ,restenosis rate was 23.5%. One narrow and contrast the age of the patients without stenosis and no statistically significant difference between lipids. The number of diabetes cases in the two comparison groups,stent length and stent volume were significant different. One bare stent restenosis rate was significantly higher than the drug-eluting stent group. Conclusion The results showed that diabetes ,stent length,multiple stents, stent restenosis,were the relevant factors of coronary restenosis.
8.Diagnostic value of combined determination of serum CA125,TSGF and DD levels in patients with ovarian cancer
Chinese Journal of Primary Medicine and Pharmacy 2010;17(23):3173-3174
Objective To investigate the clinical value ofcombined detection of the serum levels of CA125,TSGF and DD in the diagnosis of ovarian cancer. MethodsThe serum CA125 and DD were determined by enzyme linked immunosorbent assay and TSGF was determined by biochemistry shade selection in all 252 patients(including 84 cases of ovarian cancer,84 cases of ovarian benign diseases,84 cases of controls). ResultsThe concentration of CA125,TSGF and DD in the diagnosis of ovarian cancer group were higher than those in ovarian benign diseases group and the controls(all P<0.05).There was no statistical significance in the concentration of CA 125,TSGF and DD between ovarian benign diseases group and the controls(all P > 0.05).In combined assay of CA 125,TSGF and DD,the sensitivity rate was 94.05%,the specificity rate was 90.48%,and the accuracy rate was 92.26%.Comparing with the single assay,combined analysis had much higher diagnostic sensitivity(all P<0.05). ConclusionThe assay of CA125,TSGF and DD had significant dinical value in diagnosis of breast cancer,combined detection could increase the positive detection of ovarian cancer,and compensate the use of only one tumor marker.
9.How to diagnose muscular atrophy in children
Journal of Clinical Pediatrics 2009;(11):1007-1013
In clinical practice, muscular atrophy is a common sign in children. Because of relatively thick subcutaneous fat in children, muscular atrophy is not easy to be discovered. In order to confirm the diagnosis earlier, it is very important to take history in detail, to observe the motor function, cry, the posture of sitting and standing carefully, to do the physical examination thoroughly, and to use the assistant test reasonably.