Aim To study the inhibitory effect of Oxymatrine-Baicailin compound on the secretion of hepatitis B viral antigens in HepG 2.2.2.15 cells.Methods HepG2.2.2.15 cells were cultured and treated with a series of Oxymatrine,Baicailin,or Oxymatrine-Baicailin compound respectively.The toxicity effect was determined by MTT colorimetry.Con-tents of the hepatitis surface antigen(HBsAg) and hepatitis e antigen(HBeAg) in the culture supernatants were determined by Enzyme linked immunosorbent assay.Results Oxymatrine at concentrations between 0.125 and 1 g?L~(-1) had little toxicity effect on cells,but Oxymatrine at 2 g?L~(-1) and 4 g?L~(-1) had much more toxicity effect on cells.The inhibitory effect of Oxymatrine on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1).The toxicity of Baicailin on cells increased from 0.625 to 2 g?L~(-1),especially when the dose surpassed 1 g?L~(-1).The inhibitory effect of Baicailin on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1),but its efficacy was inferior to Oxymatrine's efficacy.Oxymatrine-Baicailin compound had good inhibitory effect on hepatitis B viral antigens secretion,and the inhibition effect of the compound on HBeAg was superior to the effect on HBsAg.The Group C Oxymatrine-Baicailin compound had good synergism inhibition effect on hepatitis B viral antigens secretion and the inhibition rate of the specific group compound was significantly superior to that of Oxymatrine treatment alone(HBsAg:P=0.043;HBeAg: P=0.026).Conclusion Oxymatrine-Baicailin compound has good synergism effect on hepatitis B viral antigens secretion in HepG2.2.2.15 cells.

Animals ; Breast Neoplasms ; pathology ; Cell Division ; physiology ; Cells, Cultured ; Humans ; Macrophages ; cytology ; Signal Transduction ; immunology ; physiology ; Space Flight ; Tumor Cells, Cultured ; Weightlessness

Burns ; therapy ; Fluid Therapy ; Humans

Objective To study the expressive levels of KAI-1mRNA,Kiss-1mRNA and MTA1 in the benign and malignant lesions of breast,and their clinicopathological significance.Methods We detected the expression levels of KAI-1mRNA,Kiss-1mRNA and MTA1 in the sections from 60 cases of breast cancer and 30 cases of benign lesions of breast.Results The positive rates and scores of KAI-1mRNA and Kiss-1mRNA were significantly lower in breast cancer than those in benign lesions(P

In the paper, a microtiter plate method was used to determine the residual prekallikrein activator(PKA) activity in blood products.The result of study indicated that it would be satisfactory that the reaction mixture of PKA and prekallikrein (PK) was iucul ?? at room temperature for 30 minutes with 0.05M Tris/0.15M NaCl buffer(pH 8.0) , and then the mixture continued to be incubated at room temperature for another 15 minutes after the chromogenic substrate for kallikrein was added. The method had a good reproducibility. The PKA contents determined in IVIG agreed with that of the processing method of IVIG and the hypotensive test of rats, and were basically consistent with the result, determined by the method developed by the Finnish Red Cross Blood Transfusion Service.

AlM:To analyze and explore the clinical efficacy of strengthening lateral tarsal strip and advancing lower eyelid retractor for the treatment of degenerative entropion with eyelid relaxation
METHODS: Reviewed from January 2011 to January 2013, 21 cases (33 eyes) of degenerative entropion eyelid relaxation were treated with operation of strengthening lateral tarsal strip and advancing lower eyelid retractor ( fornix conjunctival incision ) , the follow-up was 12-24mo, curative effect was observed.
RESULTS: All the patients were cured completely, have no recurrence or overcorrection phenomenon. The cure rate of operation was 100%, there was no complication.
CONCLUSlON: Operation of strengthening lateral tarsal strip and advancing lower eyelid retractor is a safe, reliable, efficient operation to the treatment of degenerative entropion with mode of eyelid relaxation.

Burns ; complications ; therapy ; Fluid Therapy ; Humans

Burns ; complications ; metabolism ; therapy ; Fluid Therapy ; Humans ; Hypoxia ; etiology ; metabolism ; prevention & control ; Ischemia ; etiology ; metabolism ; prevention & control

Objective To evaluate the clinical efficacy and toxicities of three dimensional conformal radiotherapy combined with chemotherapy for advanced esophageal carcinoma. Methods We retrospectively reviewed 127 patients with advanced esophageal squamous carcinoma in our department from January 2008 to January 2011,who were divided into two groups:conformal radiotherapy combined with chemotherapy(RCT) group,69 patients and radiotherapy(RT)group,58 patients. The side effects,quality of life and effective rates of them were compared. Results The severe rate of the marrow toxic effect and the incidences of radia-tion-induced esophagitis were higher in the RCT group(29. 0% vs 12. 1% ,χ2 = 5. 387,P = 0. 020;24. 6% vs 10. 3% ,χ2 = 4. 341,P = 0. 037). Patients in the RT group had better quality of life than those in the RCT group in gastrointestinal side effects(nausea,vomiting,diarrhea)and food intake(73. 9% vs 12. 1% ,χ2 =48. 574,P = 0. 000;43. 5% vs 25. 9% ,χ2 = 4. 274,P = 0. 039). In the RCT group,the effective rate was 91. 30%(63 / 69)and 72. 41 %(42 / 58)in the RT group,with a statistical significance(χ2 = 7. 852,P =0. 005). Conclusion Three dimensional conformal radiotherapy combined with chemotherapy for advanced esophageal carcinoma can improve the short-term curative effects,but it also increase the acute toxic effect. Further follow-up studies are needed for its long-term effect.

AIM: To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer(MIP) and Scatchard analysis was carried out. METHODS: Pueraria MIP was synthesized using pueraria as the template,acrylamide as the functional monomer and ethylene glycol dimethaerylate(EDMA) as the crosslinker. RESULTS: The result showed that at least two classes of binding sites were formed in the imprinted polymer.Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION: The component of the products is analyzed by HPLC.The result shows that the final products contain pueraria and the other two kinds of isoflavone.