Objective To explore the related high risk factors of hyponatremia after transsphenoidal approach for pituitary adenomectomy and to direct postoperative nursing. Methods The clinical data of seventy-seven patients suffering from pituitary adenomas with the way of treatment by transsphenoidal pituitary adenomectomy from June 2014 to May 2015 were summarized retrospectively. According to the value of postoperative serum natremia, the data were divided into hyponatremia group and non-hyponatremia group. The different data between the 2 groups were compared and the high risk factors related to hyponatremia were analyzed. Results Univariate analysis showed that gender(P=0.254) and high blood pressure(P=0.742) were unrelated to postoperative hyponatremia and there was significant difference between two groups in age (P=0.038), max- diameter of tumors(P=0.004), preoperative pituitary dysfunction (P=0.013), visual deficit (P=0.000), operative procedure duration (P=0.008) and diabetes mellitus rate (P=0.023). While Logistic regression analysis showed that preoperative visual deficit (OR=0.152,P=0.004,95%CI 0.043-0.542) and preoperative pituitary dysfunction (OR=0.069, P=0.046,95%CI 0.005-0.950) were independent factors for postoperative hyponatremia. Conclusions Hyponatremia is a common complication after transsphenoidal pituitary adenomectomy. The optimal treatment can benefit the early recovery and more careful nursing should be focused on these patients who are in high risk of postoperative hyponatremia.

BACKGROUND:The repair effects of bone marrow mesenchymal stem celltransplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem celltransplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury. METHODS:A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5-303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem celltransplantation group and fingolimod+bone marrow mesenchymal stem celltransplantation group. The Morris water maze test was tested at 21-28 days after PKH-26-labeled bone marrow mesenchymal stem celltransplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury. RESULTS AND CONCLUSION:At 4 weeks after transplantation, the average escape latency was gradual y decreased in each group. The average escape latency was shorter in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the bone marrow mesenchymal stem celltransplantation group (P<0.05), and significantly shorter than in the brain injury group (P<0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). Results confirmed that bone marrow mesenchymal stem celltransplantation could apparently improve memory function of rats with severe brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.

Extracellular vesicles (EV) miRNAs are a class of small non-coding RNAs that are encapsulated in membrane-containing vesicles from a variety of cells, and which can mediate intercellular communication and transfer of functional molecules. EV miRNAs in circulation can be served as novel molecular markers for cancer diagnosis, monitoring and prognosis, and also the most promising liquid biomarkers for clinical translation and utility currently, while optimal research strategy is crucial for identifying valuable EV miRNAs in clinical study. Moreover, EV miRNAs have recently also been implicated in cancer progression as direct regulators of metabolic reprogramming, mesenchymal-to-epithelial transition, angiogenesis, anti-cancer immune response and drug resistance in the tumor microenvironment. Study on some key EV miRNAs involving in those processes may highlight their potential utility as liquid biopsies in early diagnosis, companion diagnosis, metastasis, therapeutic control, prognosis analysis as their therapeutic potential for patients with cancer.

Objective To evaluate the value of liver acquisition with volume acceleration(LAVA) in the diagnosis of hepatic outflow abnormalities.Methods LAVA was performed in 78 patients with suspected hepatic outflow abnormalities,thrombosis,stenosis,occlusion,or compression,including 71 hepatocellular carcinomas and 7 Budd-Chiari syndrome.Image quality and the presence of venous abnormalities and collaterals were evaluated.Results LAVA images were of diagnostic quality and without artifact in all patients.LAVA clearly showed the vascular anatomy and various disorders.The site,extent,cause of venous obstruction,and collateral pathways were well depicted.Conclusion LAVA is simple to perform and accurate in detecting hepatic outflow abnormalities.

Objective To observe the effect of hyperglycemia on intracranial pressure (ICP) and neurological outcome following severe brain injury. Methods A retrospective review was done on 79 cases with severe traumatic brain injury (with no history of diabetes or important extracranial complications) who underwent craniotomy for evacuation of intracranial hematoma and were divided into two groups based on Glasgow outcome scales (GOS) score, ie, favorable group with GOS of 5 or 4 and unfavorable group with GOS less than 3. Statistical analysis of data was accomplished by using SPSS 11.5 software. The outcome was assessed at the 6th month after injury. Results A significant correlation was found between ICP levels and admission or postoperative glucose values. The unfavorable group had significantly higher glucose levels both on admission and after operation compared with the favorable group, with statistical difference (P

Objective To investigate the application effect of quality control management in the prevention of indwelling catheter associated urinary tract infection in intensive care unit (ICU) patients. Methods Cases of patients stayed in the department of ICU undergoing indwelling catheter over 10 d were selected by using time stage sampling method. Totally 136 cases of patients were selected from January 1, 2012 to December 31, 2012, as the control group. A total of 145 cases of patients were selected from January 1, 2013 to December 31, 2013, as the performance group. Cases in performance group were taken standardized training and quantify the performance appraisal on the basis of measures in the control group. The urine routine was tested and bacterial was cultured at indwelling catheter 3 d, 7 d and 10 d, respectively. The indwelling catheter associated urinary tract infections of the two groups were compared. Results After the implementation of the performance appraisal management, the incidences of indwelling catheter associated urinary tract infection at 3 d, 7 d and 10 d were 4.8%(7/145), 19.3% (28/145) and 32.4% (47/145), respectively. Within each quarter, the incidence of indwelling catheter associated urinary tract infection was rising with indwelling catheter time prolonged. And the incidences of the first and second quarter were higher than three and four quarter. The incidences of indwelling catheter associated urinary tract infections at 3 d, 7 d and 10 d in the performance group were lower than the control group, the differences were statistically significant (χ2=4.494, 30.660 and 49.307, P < 0.05). Conclusions Standardized training of nursing staff in ICU and implementation of performance appraisal management could effectively improve the enthusiasm and sense of responsibility of the nurses, and effectively reduce the incidence of indwelling catheter associated urinary tract infection.

OBJECTIVE: To prepare solid dispersion of berberine hydrochloride poloxamer 188. METHODS: Melting method was adopted to prepare solid dispersion. The influences of the ratio of the drug to poloxamer 188, melting temperature and cooling temperature on the dissolution of drugs were investigated. The difference of the dissolution between solid dispersion and physical mixture were explored. RESULTS: When the ratio of the drug to poloxamer 188 rose to 1 ∶ 1, the amount of the carrier was enough to distribute the drug evenly. Melting temperature had little influence on the dissolution of drugs while cooling temperature had strong influence on the dissolution of drugs. The fastest dissolution rate was achieved emerged when the cooling tempreture was 0℃. As compared with physical mixture, the dissolution rate of berberine hydroclride was nearly increased one time. CONCLUSION: Berberine hydrocloride poloxamer 188 solid dispersion improves the in vitro dissolution rate of berberine hydrocloride.

OBJECTIVE:To survey utilization of traditional Chinese medicine(TCM)combined with western medicine for TCM outpatients in our hospital and to probe into pharmaceutical care in order to decrease and prevent druginduced diseases because of irrational use of drug and provide reference for safe use of TCM combined with western medicine in the clinic. METHODS:100 cases were collected from TCM department from Jan. 1 to Dec. 31 of 2009 and their prescriptions were recorded. General information in prescriptions were recorded and analyzed. RESULTS:1 200 cases of patients there were 909 patients(75.75%) using the combination therapy,136 patients(11.33%)while use of proprietary Chinese medicines,Chinese herbal medicine and western medicine. There are 655 cases of combination therapy patients(72.06%)for rational use of medicines,while 254 patients (27.94%)are not. CONCLUSION:TCM outpatients in our hospital are treated with TCM combined western medicine in general (Chinese patent medicine combined with western medicine). There is some inappropriate use of drugs so that pharmaceutical care is necessary. Drug combination is the key to pharmaceutical care.

Objective To evaluate intravesical epirubicin instillation for preventing the recurrence of superficial bladder tumors after surgical management. Methods From Aug.1998 to Aug.1999, 32 cases of superficial bladder tumors after TURBt or partial cystectomy, received early single intravesical epirubicin instillation and the patients have been followed up for 12～24 months with cystoscopy. Results The patients followed up from 12～24 months after surgery(average time was 18 months).In 4 patients the tumor recurred 3, 6, 7 and 8 months after surgery.No side effect and complication was observed either during or after the instillation. Conclusions Early single intravesical epirubicin instillation is markedly effective for preventing the recurrence of superficial bladder tumors after surgical management. Its toxic side effects are mild. This method is of high clinical value.

Objective To study the possibility of ultr asonic examination in evaluation of cleft palate repair. Methods The length, open-close movement of soft palate and velopharyngeal insufficiency were observed by ultrasonography in submaxillary region pre-operation and post-operation in 7 patients. Results After operation- the length of soft palate was longer than that before operation, the mean extended length was 13.7- mm; the movement of soft palate was increased; the velopharyngeal insufficiency was improved. Conclusions The observation of soft palate structure pre-operation and post-operation by ultrasonic examination is an objective index for evaluating the cleft palate repair.