1.Expression of decoy receptor 3 in pancreatic cancer tissue and its clinical significance
Xinhui ZHANG ; Xin ZHAO ; Yangsui LIU ; Xiaochuan WANG ; Chenchen KONG
Chinese Journal of Hepatobiliary Surgery 2015;21(12):830-832
Objective To detect the expressions of decoy receptor 3 (DcR3) in pancreatic cancer tissues and to analyze the significance of DcR3 in the diagnosis, treatment and prognosis of patients with pancreatic cancer.Methods The expressions of DcR3 in pancreatic cancer tissues (n =100), paracancer tissues (n =15) and normal tissues (n =15) were detected with immunohistochemical method (Envision method).Results The positive rate of DcR3 in pancreatic cancer tissues was significantly higher than that in adjacent-tumor pancreatic cancer tissues (86.0% vs.46.6%, P < 0.05).The positive rate of DcR3 in adjacent-tumor pancreatic cancer tissues was significantly higher than that in normal tissues (46.6% vs.13.3%, P < 0.05).In clinical stage Ⅲ, the positive rate of DcR3 was significantly higher than that in stage Ⅱ and stage Ⅰ (100% vs.87.0%, P<0.05;100% vs.62.5%, P<0.05).There were significant differences among the three groups (P < 0.05).With lymph node metastasis, the DcR3 positive rate was significantly higher than that in the group without lymph node metastasis (93.4% vs.79.6%, P < 0.05).In poorly differentiated pancreatic cancer, the positive rate of DcR3 was significantly higher than that in the highly differentiated group (100% vs.64.0%, P <0.05), the positive rate of DcR3 was significantly higher in the moderately differentiated group than that in the highly differentiated group (88.6% vs.64.0%, P < 0.05) , There were significant differences among the three groups (P < 0.05).There was no significant difference in the positive rate of DcR3 between the different age groups or the different gender groups (P > 0.05).Conclusions The expression levels of DcR3 in patients with pancreatic cancer gradually increased from normal tissues to paracancer tissues, to pancreas tissues.The expression level of DcR3 protein was closely related to clinical stage, degree of tissue differentiation and presence of lymph node metastasis, but not associated with age, sex, and tumor diameter size.
2.The study of Sirt1 promotes the osteogenic differentiation of inflammatory PDLSCs
Xiangwei Kong ; Wei Yin ; Chenchen Wang ; Lin Zhang ; Yicheng Cheng
Acta Universitatis Medicinalis Anhui 2023;58(5):876-880
Objective:
To study the positive effect and mechanism of histone deacetylase Sirtuin 1 (Sirt1) on the osteogenic capacity of inflammatory PDLSCs.
Methods:
PDLSCs were isolated and cultured from the healthy individuals and periodontitis patients , the expression of Sirt1 in two types of PDLSCs was observed;osteogenic induction of inflammatory PDLSCs was performed , concurrently Sirt1 agonist resveratrol was used to up⁃regulate the expression of Sirt1 , then the osteogenic differentiation was observed , the expressions of Runx2 , acetylated NF⁃κB , acetylated FoxO1 and FoxO1 were assessed by Western blot.
Results :
The protein expression of Sirt1 was suppressed in in flamed PDLSCs( P < 0. 01) ;resveratrol could up⁃regulate the expression of Sirt1 in inflamed PDLSCs;compared with the osteogenic induction group , the osteogenesis + resveratrol group increased the expression of BSP ( t =14. 045 ,P < 0. 01) , Runx2( t = 3. 349 ,P < 0. 01) , OCN( t = 7. 218 ,P < 0. 01) and Osx ( t = 4. 544 ,P < 0. 01) mRNA in inflamed PDLSCs , and enhanced ALP staining(P < 0. 01) ;expression of FoxO1( t = 8. 737 , P < 0. 01) and Runx2( t = 6. 152 , P < 0. 01) increased after osteogenesis of inflamed PDLSCs ; in the osteogenesis + resveratrol
group , the expression of Sirt1 was up⁃regulated , and the expression of FoxO1( t = 5. 912 ,P < 0. 01) and Runx2( t =6. 277 ,P < 0. 01) further increased compared with the osteogenic induction group ,while the expression of acetylated NF⁃κB(F = 184. 033 ,P < 0. 01) and acetylated FoxO1(F = 301. 454 , P < 0. 01) was significantly lower than that of control group and osteogenic induction group.
Conclusion
The deacetylase Sirt1 could promote the osteogenic differentiation of inflammatory PDLSCs through deacetylating NF⁃κB and FoxO1 .
3.Construction and optimization of automatic checking mode for unit dose dispensing system of oral drugs
Tingting QIU ; Yingying YAN ; Yuchen ZHANG ; Chenchen ZHANG ; Yinchu CHENG ; Xianwei KONG ; Yiheng YANG
China Pharmacy 2023;34(16):2018-2021
OBJECTIVE To provide a reference for establishing an automatic checking mode and improving the checking efficiency of the unit dose dispensing system of oral drugs in hospital. METHODS The automatic checking process reengineering team was established in our hospital. ECRSI method was adopted to sort out the verification process and mode of drug bags for the unit dose formula of our hospital through five principles of eliminating, combining, rearranging, simplifying and increasing, and the hardware series problem and the problem of excessive system false-positive proportion were optimized. The drug bags for the unit dose formula were randomly selected from 10 wards, the efficiency and external error rates of manual check and automatic checking mode before and after optimization were compared, and the false-positive reporting failure in automatic checking mode was also compared before and after optimization. RESULTS After the establishment of the automatic checking mode of the unit dose formula for oral drugs, the average checking time of drug bags was significantly shorter than that of manual checking mode in the other 8 wards except for cardiovascular and renal departments (P<0.05). After the optimization of the automatic checking mode, the average checking time of drug bags in all wards was significantly shorter than that in manual checking mode (P<0.05). Compared with before optimization of the automatic checking mode, the average checking time of drug bags was shortened by 0.43 s, and the average checking time of drug bags in half of the wards was shortened significantly (P<0.05). At the same time, the false-positive proportion decreased from 96.83% before optimization to 92.76% after optimization (P<0.05). The external error rate dropped from 0.039‰ in manual checking mode to 0.019‰ before optimization and 0.015‰ after optimization (P<0.05). CONCLUSIONS Based on ECRSI method, the automatic checking mode for the unit dose dispensing system of oral drugs can effectively reduce the average checking time of drug bags, reduce external error and improve the work efficiency of pharmacists.
4.Downregulation of cardiac PIASy inhibits Cx43 SUMOylation and ameliorates ventricular arrhythmias in a rat model of myocardial ischemia/reperfusion injury.
Tingting WANG ; Jinmin LIU ; Chenchen HU ; Xin WEI ; Linlin HAN ; Afang ZHU ; Rong WANG ; Zhijun CHEN ; Zhengyuan XIA ; Shanglong YAO ; Weike MAO
Chinese Medical Journal 2023;136(11):1349-1357
BACKGROUND:
Dysfunction of the gap junction channel protein connexin 43 (Cx43) contributes to myocardial ischemia/reperfusion (I/R)-induced ventricular arrhythmias. Cx43 can be regulated by small ubiquitin-like modifier (SUMO) modification. Protein inhibitor of activated STAT Y (PIASy) is an E3 SUMO ligase for its target proteins. However, whether Cx43 is a target protein of PIASy and whether Cx43 SUMOylation plays a role in I/R-induced arrhythmias are largely unknown.
METHODS:
Male Sprague-Dawley rats were infected with PIASy short hairpin ribonucleic acid (shRNA) using recombinant adeno-associated virus subtype 9 (rAAV9). Two weeks later, the rats were subjected to 45 min of left coronary artery occlusion followed by 2 h reperfusion. Electrocardiogram was recorded to assess arrhythmias. Rat ventricular tissues were collected for molecular biological measurements.
RESULTS:
Following 45 min of ischemia, QRS duration and QTc intervals statistically significantly increased, but these values decreased after transfecting PIASy shRNA. PIASy downregulation ameliorated ventricular arrhythmias induced by myocardial I/R, as evidenced by the decreased incidence of ventricular tachycardia and ventricular fibrillation, and reduced arrythmia score. In addition, myocardial I/R statistically significantly induced PIASy expression and Cx43 SUMOylation, accompanied by reduced Cx43 phosphorylation and plakophilin 2 (PKP2) expression. Moreover, PIASy downregulation remarkably reduced Cx43 SUMOylation, accompanied by increased Cx43 phosphorylation and PKP2 expression after I/R.
CONCLUSION
PIASy downregulation inhibited Cx43 SUMOylation and increased PKP2 expression, thereby improving ventricular arrhythmias in ischemic/reperfused rats heart.
Rats
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Male
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Animals
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Myocardial Reperfusion Injury/metabolism*
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Connexin 43/genetics*
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Sumoylation
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Down-Regulation
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Rats, Sprague-Dawley
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Arrhythmias, Cardiac/drug therapy*
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Myocardial Ischemia/metabolism*
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RNA, Small Interfering/metabolism*