Acute kidney injury(AKI) can lead to remote organ damage.The specific mechanism by which the AKI results in distant organ damage is yet to be determined.In animal models,it has become increasingly clear that AKI is not an isolated event but results in remote organ dysfunction involving the heart,lungs,liver,and brain.This review summarizes the effects of AKI on remote organs.

The zymograms of lactate dehydrogenase (LDH) and esterase (EST) isoenzymes in different physiologic state from two species of Anopheles mosquitoes were studied with polyacrylamide gel electro -phoresis. Some zymogram variation of LDH from single female mosquito was found after engorged blood meals for 48 h, that consisted of two main bands whose relative mobility (Rm) was different. The isozymograms of EST showed some similar characteristics of sibling which divided into three groups ESTⅠ, ESTⅡand ESTⅢ, respectively. The two species of mosquitoes could be differentiated from each other by the particular band in EST Ⅱ group of An. anthropophagus.

A simple and reliable procedure for the isolation of pure DNA from human fetal liver is described.A high pure and natural DNA with an average molecular weight of 4 ?106 can be easily obtained after the sample is treated with SDS and trypsin and fractionated by a sepharose 4B column.The product meets the requirement for general research work of molecular biology.

This paper is to report the results of the clinical application of the Shexiang hemostasis-removing plaster. 265 cases of dermatohemostasis including chilblain, erythema multiforme, erythema nodosum, and so on were treated locally with Shexiang hemostasis-removing plaster. 20 cases of dermatohemostasis were treated with Shexiang analgesic plaster and served as control.It was found the rates of marked improvement and improvement after the application of Shexiang hemostasis-removing plaster were 85.61% and 96.60%, while those after Shxiang analgesic plaster treatment were 65% and 90% respectively. It is bovious that Shexiang hemostasis-removing plaster is more effective in treating dermatohemostasis than Shexiang analgesic plaster. The difference between the 2 is statistically significant(P

Coronal restoration of endodontically treated teeth may be viewed as one of the main parameters that influence the outcome of endodontic treatment. The purposes of restoring endodontically-treated teeth include preventing recontamination of the root canal system and periapical area, replacing the compromised dental hard tissue, restoring the coronal morphology and function, providing necessary strength for the restoration/tooth complex for functional stress, and avoiding crown and/or root fracture. This article reviewed recent researches on the restoration of endodontically treated teeth, provided evidence for clinical practice on topics as when to restore them, basic principles to be considered during treatment planning, and specific restoration options for both anterior and posterior teeth under different functional occulsal load conditions. Several issues should be taken into account during the decision making process, such as remaining tooth tissue, functional masticatory forces, comprehensive oral rehabilitation, and esthetic requirements.
Decision Making ; Dental Restoration, Permanent ; Humans ; Root Canal Therapy ; Tooth Crown ; Tooth, Nonvital

Zebrafish has unique advantages over other animal models in the aspect of drug screens for active ingredients and gains more and more attentions in drug research and development recently. Thus, this article reviews the recent advance of zebrafish-based drug screens in Chinese traditional medicine (TCM) effective part research, monomer drug screening, activity evaluation of natural products, discovery of new uses for old drugs, and toxicity assessment in early-phase drug discovery.
Animals ; Drug Evaluation, Preclinical ; instrumentation ; methods ; Drugs, Chinese Herbal ; pharmacology ; Models, Animal ; Zebrafish ; growth & development

The International Association for the Study of Lung Cancer (IASLC) has presented the details of the IASLC/International Union Against Cancer (UICC)/American Joint Committee on Cancer (AJCC) Revised Staging Classification for Lung Cancer. The IASLC is the largest world-wide professional organization solely dedicated to reduce the worldwide burden of lung cancer. The IASLC recognizes that the staging classification will be most valuable and accurate if it is based on the evaluation of outcomes of large numbers of cases carefully collected and analyzed in an extensive worldwide database. The analyses of the T, N and M descriptors as well as the stage groupings were performed in 67,725 non-small cell lung cancer (NSCLC)patients. Survival was the primary outcome, measured from the date of diagnosis or date of protocol registration for clinical staging, or the date of surgery for pathologic staging. The remarkable efforts of the IASLC Staging Committee have resulted in an evidence-based, validated and robust revision of the international staging system for NSCLC. This landmark contribution will improve our care of patients and lays a strong foundation for future refinements based on an expanding knowledge of lung cancer behavior and biology. This review outlines the changes in the tumor, node, metastasis (TNM) descriptors and stage groupings anticipated in the official new stage classification system for NSCLC.

Adolescent ; Age Factors ; Biopsy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Kidney ; diagnostic imaging ; pathology ; physiopathology ; Kidney Failure, Chronic ; etiology ; pathology ; Male ; Multicystic Dysplastic Kidney ; complications ; Nephritis ; complications ; Nephrotic Syndrome ; complications ; Radiography

Objective To investigate the the effect and interaction of morphine dependence model and naloxone doses on withdrawal intensity. Methods To observe the withdrawal signs and weight loss in two kinds of morphine dependent rats after the precipitating by naloxone of different doses an devaluate the withdrawal intensity.Results Morphine dependence were successfully induced in two ways.There was no significant difference of withdrawal intensity in five-day model(total morphine dose 380mg.kg－1) after precipitating by naloxone of 2mg.kg－1 and 4mg.kg－1（P＞0.05）. Withdrawal intensity of twelve-day model(total morphine dose 1365mg.kg－1)increased significantly with the increase of naloxone dose（P＜0.01）,but it was more intensive significantly than five-day model only after 4mg.kg－1 dose of naloxone. Conclusion Appropriate method should be used to induce morphine dependence and these experimental factors should be combined in assessment of withdrawal intensity.

Objective To explore different pathogenesis of chronic hepatitis B(CHB)and asymptomatic H BV carriers(ASCs)by identifying differentially expressed genes.Methods Subtracted library was constructed by suppression subtraetive hybridization(SSH),and α-defensin was identified by dot blot hybridization.Peripheral blood was collected from 46 CHB patients and 11 ASCs.and the expressions of α-defensin mRNA in peripheral blood mononuclear cell and protein in plasma were determined by the real time RT-PCR and enzyme-linked immunosorbent assay(ELISA).Results Real time RT-PCR showed that the expression of α-defensin mRNA in blood samples of CHB was 1.4-fold higher than that of ASCs.As shown by ELISA,the plasma level of α-defensin in CHB was higher than that of ASCs [(216.40±81.25)μg/L vs.(156.00±57.26)μg/L,t=2.23,P<0.05].Conclusion α-defensin may involve in the pathogenesis of CHB,for it iS over-expressed in CHB patients.