The zymograms of lactate dehydrogenase (LDH) and esterase (EST) isoenzymes in different physiologic state from two species of Anopheles mosquitoes were studied with polyacrylamide gel electro -phoresis. Some zymogram variation of LDH from single female mosquito was found after engorged blood meals for 48 h, that consisted of two main bands whose relative mobility (Rm) was different. The isozymograms of EST showed some similar characteristics of sibling which divided into three groups ESTⅠ, ESTⅡand ESTⅢ, respectively. The two species of mosquitoes could be differentiated from each other by the particular band in EST Ⅱ group of An. anthropophagus.

A simple and reliable procedure for the isolation of pure DNA from human fetal liver is described.A high pure and natural DNA with an average molecular weight of 4 ?106 can be easily obtained after the sample is treated with SDS and trypsin and fractionated by a sepharose 4B column.The product meets the requirement for general research work of molecular biology.

This paper is to report the results of the clinical application of the Shexiang hemostasis-removing plaster. 265 cases of dermatohemostasis including chilblain, erythema multiforme, erythema nodosum, and so on were treated locally with Shexiang hemostasis-removing plaster. 20 cases of dermatohemostasis were treated with Shexiang analgesic plaster and served as control.It was found the rates of marked improvement and improvement after the application of Shexiang hemostasis-removing plaster were 85.61% and 96.60%, while those after Shxiang analgesic plaster treatment were 65% and 90% respectively. It is bovious that Shexiang hemostasis-removing plaster is more effective in treating dermatohemostasis than Shexiang analgesic plaster. The difference between the 2 is statistically significant(P

Acute kidney injury(AKI) can lead to remote organ damage.The specific mechanism by which the AKI results in distant organ damage is yet to be determined.In animal models,it has become increasingly clear that AKI is not an isolated event but results in remote organ dysfunction involving the heart,lungs,liver,and brain.This review summarizes the effects of AKI on remote organs.

Coronal restoration of endodontically treated teeth may be viewed as one of the main parameters that influence the outcome of endodontic treatment. The purposes of restoring endodontically-treated teeth include preventing recontamination of the root canal system and periapical area, replacing the compromised dental hard tissue, restoring the coronal morphology and function, providing necessary strength for the restoration/tooth complex for functional stress, and avoiding crown and/or root fracture. This article reviewed recent researches on the restoration of endodontically treated teeth, provided evidence for clinical practice on topics as when to restore them, basic principles to be considered during treatment planning, and specific restoration options for both anterior and posterior teeth under different functional occulsal load conditions. Several issues should be taken into account during the decision making process, such as remaining tooth tissue, functional masticatory forces, comprehensive oral rehabilitation, and esthetic requirements.
Decision Making ; Dental Restoration, Permanent ; Humans ; Root Canal Therapy ; Tooth Crown ; Tooth, Nonvital

Zebrafish has unique advantages over other animal models in the aspect of drug screens for active ingredients and gains more and more attentions in drug research and development recently. Thus, this article reviews the recent advance of zebrafish-based drug screens in Chinese traditional medicine (TCM) effective part research, monomer drug screening, activity evaluation of natural products, discovery of new uses for old drugs, and toxicity assessment in early-phase drug discovery.
Animals ; Drug Evaluation, Preclinical ; instrumentation ; methods ; Drugs, Chinese Herbal ; pharmacology ; Models, Animal ; Zebrafish ; growth & development

Child ; Chronic Disease ; Cough ; diagnosis ; therapy ; Guidelines as Topic ; Humans ; Pediatrics

Toxicogenomics is a new undertaking in the pursuit of human genomics relevant to health risk from environmental toxicants and related stress, and also is a new scientific subdiscipline derived from a combination of the fields of toxicology and genomics. Microarray technique, i. e. gene-chip technique includes gene-chip development, pretreatment of detected samples, probe hybridization, detection data analysis. The microarray technique in toxicology mainly was applied to identification of potential hazardous substances, screening for mechanism of action, dose-response relationship assessment, identification of interaction among chemical mixtures, biomarker of exposure and biomarker of susceptibility. Microarray technique has been used to study biomarker for human bladder cancer, to study fingerprints of gene regulation associated with cadmium chloride, benzo(a) pyrene and trichloroethylene. fn this paper, a new automated and multi-applied molecular biology workstation, NanoChip biomolecular system was introduced .

Objective To investigate the the effect and interaction of morphine dependence model and naloxone doses on withdrawal intensity. Methods To observe the withdrawal signs and weight loss in two kinds of morphine dependent rats after the precipitating by naloxone of different doses an devaluate the withdrawal intensity.Results Morphine dependence were successfully induced in two ways.There was no significant difference of withdrawal intensity in five-day model(total morphine dose 380mg.kg－1) after precipitating by naloxone of 2mg.kg－1 and 4mg.kg－1（P＞0.05）. Withdrawal intensity of twelve-day model(total morphine dose 1365mg.kg－1)increased significantly with the increase of naloxone dose（P＜0.01）,but it was more intensive significantly than five-day model only after 4mg.kg－1 dose of naloxone. Conclusion Appropriate method should be used to induce morphine dependence and these experimental factors should be combined in assessment of withdrawal intensity.

Objective To screen immune molecules expressed on peripheral blood mononuclear cells (PBMCs) which arc related to the aggravation of chronc hepatitis B (CHB). Methods Real-time polymerase chain reaction (PCR) was employed to detect the relative expression of 39 immune molecules at mRNA level and then compare the differences between groups (control group, mild CHB group, moderate CHB group and severe CHB group). The investigated immune molecules included leukocyte differentiation antigens, chemokine receptors, apoptosis-related ligands and receptors,adhesion molecules and Toll like receptors. Scheffe model was used to compare the differences among all groups and Spearman rank correlation was used for correlation analysis. Results Among the 39 immune molecules, the expressions of tumor necrosis factor related apoptosis inducing ligand (TRAIL), TRAIL receptor (TRAIL-R) 2, CD64, CD30, CD27, CD28, L-selectin (CD62L),intercellular adhesion molecule-1 (ICAM-1), chemokine (C-X-C motif) receptor (CXCR) 2 were significantly increased in severe CHB group compared with those in control group ( 1.96, 2.13, 1.33,1.16, 1.57, 2.14, 2.03, 2. 10 and 2.09, respectively; all P＜0.05). The expressions of TRAIL,TRAIL-R2, CD64, CD30, CD27 were highly correlated with the expression of interferon gamma (IFN-γ) (r=0. 816, 0. 572, 0. 462, 0. 697 and 0. 793, respectively; all P＜0.01). The expressions of TRAIL-R2, CD64, CD30, CD62L, ICAM-1 were highly correlated with the expression of tumor necrosis factor alpha (TNF-α) (r=0. 494, 0. 588, 0. 568, 0. 968 and 0. 976, respectively; all P＜0.01). Conclusion The abnormal expression of TRAIL,TRAIL R2,CD64,CD30,CD27,CD28,CD62L,ICAM-1 and CXCR2 may play an important role in the pathogenesis and aggravatoin of hepatitis B.