AIM:To investigate the effects of tanshinone IIA ( Tan) on cardiac action potential in rats with a-cute cerebral ischemia (ACI).METHODS:ACI was established in rats accordingly .Animals were divided into 3 groups:sham group, ACI group, and ACI with Tan treatment group .The defect of neural function in each group was graded , elec-trocardiogram was measured , monophasic action potential was recorded , and the levels of cardiac troponin I ( cTnI ) and creatine kinase-MB ( CK-MB) were examined by commercially available kits .RESULTS:Compared with ACI group , Tan significantly decreased the scores of the defect of neural function , reduced the incidence and duration of the abnormalities in electrocardiogram , prolonged the effective refractory period , action potential duration at 50%repolarization and action po-tential duration at 90%repolarization, and inhibited the elevation of cTnI and CK-MB levels.CONCLUSION:Tan main-tains a stable heart rhythm in ACI rats , which may be related to its protective effect on ischemic myocardium .

ObjectiveTo investigate the outcomes of mother a nd baby smaller than 37 gestational age caused by preeclampsia. Method sThe data for 122 preterm birth that was single pregnancy with preecla mpsia in our hospital from January 1993 to December 2002 were collected. The pri nciple of diagnosis and treatment, the outcomes were analyzed retrospectively. T he 122 pregnant women were divided into 3 groups according to their gestational age, more than 28 weeks and smaller than 31 +6 weeks was the first group (28 cases), more than 32 weeks and smaller than 33 +6 weeks was the second grou p (26 cases), more than 34 weeks and smaller than 36 +6 weeks was the thir d group (68 cases). The data were statisted with SAS software.Results The earlier the pregnancy-induced hypertension happened, the earlier the preeclampsia occured, the mean time was 14 03 d, 16 85 d,22 22 d, respect ively, (P0.05), the outcomes of mothers were good. Increasing the gestational age under the cond ition that the occurrene of comptications was not increased, 60 64% pregnant wo men accepted the treatment of improving the maturity of fetal lung. There were n o differences among the three groups at the morbidity of perinatal SGA, intracra nial hemorrhage (P 0.05) .ConclusionWe could improve the outcomes of pret erm birth caused by preeclampsia by enhancing the perinatal care, treating and t erminating pregnancy activey Increasing the gestational age, improving the matur ity of fetal lung would decrease the morbidity of severe RDS and improve the pe rinatal outcome effectively.

Objective: To explore the effect of traumatic asphyxia on mental health of adolescents. Method: Forty-four adolescents were assessed with Styles Questionnaire (SCL-90), Eysenck Personality Questionnaire (EPQ) after one week, 6 weeks, 10 weeks, 16 weeks and 34 weeks of asphyxia. Post-traumatic Stress Disorder (PTSD) was diagnosed according to CCMD-2-R. Results: All 44 adolescents suffered from traumatic asphyxia had some psychiatric symptoms. Nineteen of them fulfilled the symptomatic criterta of DTSD in the first week,10 of them were diagnosed as PTSD at the 6th week,8 of the them returned to normal after 34 weeks. Conclusion: Mental health of adolescents suffered from traumatic asphyxia need our attention.

Objective To study far femoral malignant bone tumor salvage limb treatment with allogeneic heterotopic half-joint transplantation and the use of GSH nail Methods Eleven cases with far femoral malignant bone tumor were administered tumor resection,transplanted with allogeneic heterotoeic bone half-joint,and fixed with GSH nail Results Nine out of the 11 cases were followed up.None of them had infection,rejection or heterotopic bone fracture.All of them can move with walking after 6 months.The effects of surgery was rated as excellent for 3 cases,fine for 4 cases andbad for 2 cases according to Mankin's rating criteria.Conclusion Salvage limb treatment for bone tumor can improve patients' quality of life.Allogeneic heterotopic bone,with rich source and acceptable joint surface,is a good graft for the treatment of bone defect after bone tumor resection.

Objective:To observe the clinical treatment effects of Tangkang Xifang in the patients with type 0 diabetes foot ( DF) . Methods:Totally 73 patients with type 0 DF were randomly divided into the treatment group (n=37) and the control group (n=36). All the patients discontinued other medications for 7d before the treatment. Base on the blood glucose control with the traditional treat-ment by metformin hydrochloride (1. 5 g·d-1), the treatment group was additionally given warm water foot bath with Tangkang Xi-fang, while the control group was additionally given warm water foot bath, 30 min per time and twice a day for 60 days. The changes in the clinical symptoms, ankle brachial index ( ABI) , motor nerve conduction velocity ( MCV) and some other indices were measured before and after the treatment. Results:In comparison with the control group (58. 3%), the treatment group had higher total effective rate (86. 5%), and the usage of Tangkang Xifang also significantly improved the physiopathologic syndromes of peripheral artery dis-ease and neuropathy (P<0. 01). The ABI and MCV in the treatment group were significantly better than those in the control group (P<0. 05 or P<0. 01). Conclusion:Tangkang Xifang can effectively improve the clinical symptoms of type 0 DF, which is suitable for the clinical application.

Objective To observe the effects of posterior screw and rod fixation on nerve function and vertebral indicators in patients with lumbar vertebral fracture.Methods The clinical data of 32 cases with lumbar vertebral fracture were analyzed retrospectively.According to Frankle grading:grade A 1 case ;grade B 7 cases;grade C 12 cases ;grade D 12 cases.All the patients were treated by posterior screw and rod fixation.The nerve function and vertebral indicators were compared.Results Followed-up for 22.3 months,according to Frankle grading:grade A 1 case;grade B 3 cases;grade C 9 cases;grade D 15 cases;grade E 4 cases.The nerve functions were improved after operation.There was a significant difference between before and after treatment (all P ＜ 0.05).The the height of the front edge of the fractured vertebral and cobb angle were improved after operation (all P ＜ 0.01).Conclusion Posterior screw and rod fixation can significantly improve nerve function and vertebral indicators in patients with lumbar vertebral fracture.

Objective To investigate the value of interleukin-6 (IL-6) level as a prognostic indicator in diabetic ketoacidosis (DKA) patients.Methods Data of 88 cases of DKA in Sir Run Run Shaw Hospital collected from June 1,2010 to December 31,2012 were analyzed prospectively.They were divided into two groups according to the survival of patients followed up for 28 days:non-survival group (n =12) and survival group (n =76).Serum levels of IL-6,lactate,base excess and APACHE Ⅱ score in 24 hours after admission were compared between two groups by independent t test.The receiver operating characteristic (ROC) curve was plotted to analyze the performance of IL-6 as a prognostic indicator in the early stage of DKA.Correlations between IL-6 value and lactate,base excess,APACHE Ⅱ score were analyzed.Results The IL-6 values were elevated obviously in non-survival group.The ROC of IL-6 had an area under curve (AUC) of 0.783,and the cut-off value of IL-6 for the death followed up for 28 days was 448.9 ng/L.In all patients,IL-6 values and APACHE Ⅱ score showed a positive correlation between them (r =0.919,P =0.005),and also positive correlations existed between IL-6 and lactate as well as base excess (r=0.922,P=0.003; r=0.887,P=0.015)in24 hours.Conclusions IL-6 value is a reliable prognostic indicator in the early stage of DKA.

Hypogonadotropic hypogonadism (HH) is a heterogeneous group of diseases with variable clinical manifestations.Currently,the primary treatment of HH is hormone replacement therapy,including testosterone replacement for induction of pubertal development and gonadotropin therapy or pulse GnRH administration for induction of spermatogenesis.

Microorganism distributes in the organs of human body which connect with external environment, especially those organs in the gastrointestinal tracts, and it also plays a fundamental role in the physiopathology of the host's body. Because the microorganism is very small and has a great variety, it is difficult to reveal the significance of microorganism in the human physiopathology comprehensively and deeply. With the development of molecular biology, genomics, bioinformatics and other disciplines, the microbiome research will be more possible and easier. There are two key contents of microecology. One of these is to identify and quantify the diversity of microorganism, and the other is to reveal activity and the physiopathological function of microorganism in the host. Microbiome research methods, therefore, can be summarized as the traditional detection methods, construction of gene library, the genetic fingerprint analysis and molecular hybridization techniques and so on.
Computational Biology ; Gastrointestinal Microbiome ; Gastrointestinal Tract ; microbiology ; Humans

Emergency gastroscopy was performed for 146 patients with upper gastrointestinal hemorrhage admitted to Tieying Hospital, Beijing to identify causes of bleeding and establish diagnosis. One hundred and thirty-nine of them were cured with endoscopic treatment, but two failed so to change to surgical operation. It suggested that early gastroscopy is a convenient, easy and safe method in diagnosis and treatment of upper gastrointestinal hemorrhage.