Objective:To investigate prevalent aeroallergens in ChongQing and their sensitization risk factors in children with allergic diseases,especially asthma.Methods:Skin prick tests with 13 standardized aeroallergens and questionnaires were used.A total of 298 patients,aged between 4~15 years and were diagnosed with allergic asthma and or allergic rhinitis,were investigated.Results:The total positive rate of allergen was 84.9%(253/298).The prevalence of aeroallergens were following:the first was house dust mite,cockroach followed,the third one was cat dander;the positive family history of asthma was 69.6%(116/230),asthma and rhinitis accounted for 67.2%(78/116)。Multivariate logistic regression analyses revealed that house dust mite was an independent risk factors for asthma(odds ratio Value(OR):2.602,95%CI:1.672～7.488),air conditioning had strong relationship with it(OR:1.659,95%CI:1.139～5.942).Conclusion:House dust mite is the major allergen of allergic diseases,especially asthma in ChongQing.Air conditioning is one of important risk factors for house dust mite sensitization.

Objective:To prepare Pingwei dispersible tablets and establish a method for the determination of atractylodin. Meth-ods:HPLC was performed on a Kromasil C18 column(250 mm × 4. 6 mm,5 μm)using methanol-water(75:25)as the mobile phase at a flow rate of 1 ml·min-1 . The detection wavelength was at 340nm. The column temperature was 30℃ and the injection volume was 10 μl. Results:The optimized formula of the tablets was as follows:the proportion of inner disintegrant microcrystalline cellulose,out-side disintegrant carboxymethyl cellulose sodium and HPMC was 40%,9% and 2%,respectively. The adhesive was 2% HPMC in 70% ethanol,and 1. 5% magnesium stearate was used as the lubricant. The linear range of atractylodin was 0. 100 6-0. 503 0 μg(r=0. 999 9),and the average recovery was 99. 3%(RSD=0. 9%,n=6). Conclusion:The formula and the preparation process of the tablets are reasonable and the content determination method is simple,accurate and specific,which can be used in the quality control of Pingwei dispersible tablets.

Coronary Artery Disease ; diagnosis ; therapy ; Humans ; Tomography, Optical Coherence ; methods ; Treatment Outcome

Posterior capsule opacification(PCO) is a common postoperative complication of cataract surgery.PCO is usually caused by the proliferation,migration,epithelial-to-mesenchymal transition (EMT),collagen deposition,and lens fiber regeneration of residual lens epithelial cells(LECs).Transforming growth factor-β(TGF-β) has been proposed as the most important factor driving the trans-differentiation and pathologic fibrosis of LECs.A variety of studies have elucidated that the Smad signaling pathway plays a critical role in the transduction of TGF-β signal.However,there are still other signaling pathways which may be activated downstream by TGF-β,including phosphatidylinositol-3-kinase/Akt(PI3 K/Akt),Rho,Wnt and mitogen-activated protein kinases (MAPK) pathways.This review highlights recent advances in the study of the signaling pathway of TGF-β in the pathological process of PCO.

The changes of immune system following stroke mainly includes the local inflammatory responses of brain tissue and the systemic immunological changes, The former may exacerbate brain damage following stroke, the latter may increase the susceptibility to infection, and thus impact on brain function recovery and prognosis. Stroke interferes with the normal balance between the nervous system and the immune system, and its mechanism remains unclear. This article reviews the stroke-indnced immunological alterations and their mechanisms,as well as the corresponding treatment strategies in clinical practice.

Superficial siderosis of the central nervous system is a seldom disease in clinical practice. Its most common clinical manifestations are hearing loss and ataxia. It is a hemosiderin deposition in the surface of the brain and spinal cord after chronic subarachnoid hemorrhage. Magnetic resonance imaging can make the diagnosis. However, further research and clinical verification are needed.

Cerebral small vessel disease (SVD) is an important subtype of cerebrovascular disease. It is also one of the major reasons for resulting in vascular cognitive impairment or dementia in the elderly. SVD is a small arteriovenous lesion in subcortex caused by a variety of causes, mainly causing subcortical lacunar infarction, white matter damage, microblecds and other pathological changes. There is evidence that vascular endothelial function and blood-brain barrier damage may result in small vessel structures and perivascular changes, which may be the initial factors.of causing SVD. Genetic susceptibility is also one of the risk factors that can not be ignored.

Objective To investigate the clinical efficacy and safety of kidney stones minimally invasive percutaneous nephrolithotomy holmium laser lithotripsy.Methods 397 cases patients with kidney stones were randomly divided into the control group and the experimental group,the control group were treated with the extracorporeal shock wave lithotripsy treatment,and the later group were treated with holmium laser lithotripsy for percutaneous nephrolithotomy.Results The experimental group of taking the net rate was 97.48％,and the time of taking the net rate was 87.15 ％,higher than those of the control group(x2 =14.72,24.78,all P ＜ 0.05).Compared with the control group,the shortened operation time,(t =2.36,P ＜ 0.05),the reducing amount of bleeding (t =3.27,P ＜ 0.05),the shortened length of stay hospital (t =1.28,P ＜ 0.05),low complication rate (x2 =17.06,P ＜ 0.05) took place in the experimental group.Conclusion Minimally invasive percutaneous nephrolithotomy holmium laser lithotripsy treatment of kidney stones is safe and effective,fewer complications,faster recovery,which is worthy of promotion.

The patients with stroke is often accompanied by malnutrition, its causes include eating less, increased catabolism, and intestinal dysfunction, etc. Malnutrition can not only aggravate the extent of brain damage in patients with stroke, but also increase the incidence of complications, and thus affecting the course and prognosis of stroke. Therefore, correct evaluation of the nutritional status in stroke patients, choosing the appropriate nutrient timing and nutritional support methods have important significance. Among a variety of nutritional assessment methods, the scored Patient-Generated Subjective Global Assessment can quickly identify malnutrition in stroke patients. Early enteral nutrition support, including oral nutritional supplements, nasogastric tube feeding and percutaneous endoscopic gastrostomy, can significantly improve the prognosis in stroke patients. Among them, nasogastric tube feeding is the first choice for nutritional support in stroke patients.

Objective To investigate the effects of CO 2 pneumoperitoneum during laparoscopic cholecystectomy (LC) on pulmonary functions in chronic pulmonary failure rabbits and their mechanisms. Methods A total of 50 healthy male rabbits ( oryctolagus cuniculus ) were randomly divided into 4 groups: normal control group (N 0: n=5, no pneumoperitoneum), experimental control group (T 0: n=5, no pneumoperitoneum), 10 mmHg experimental group (T 10 : n=20, 10 mmHg pneumoperitoneum) and 15 mmHg experimental group (T 15 : n=20, 15 mmHg pneumoperitoneum). After the successful establishment of emphysema rabbit models, CO 2 pneumoperitoneum was conducted and maintained for 2 hours at the pressure of 10 mmHg (1.33 kPa) and 15 mmHg (2.00 kPa), respectively. Pulmonary functions (total respiratory resistance, central resistance and total airway resistance) of the 4 groups were measured by pulse oscillation technique before and after the pneumoperitoneum, respectively. Results Total respiratory resistance, central resistance and total airway resistance increased after the establishment of emphysema models. And they decreased at the end of pneumoperitoneum compared with those before pneumoperitoneum ( q=17.824, P