Objective To evaluate the therapeutic efficacy of percutaneous transhepatic portal vein catheterization and thrombolysis on acute superior mesenteric vein thrombosis.Methods The treatment and therapeutic efficacy of 7 cases of acute superior mesenteric vein thrombosis underwent percutaneous transhepatic portal vein catheterization and thrombolysis under ultrasound guidance from August 2005 to April 2009 were analyzed.Results All the patients succeeded in portal vein catheterization and no bile leakage or abdominal bleeding occurred during the procedure.The clinical symptoms such as abdominal pain,abdominal distension,and passing bloody stool relieved were relieved and liquid diet began at postoperative of day 2-5.Emergency operation was done in one case and there was no intestinal fistula.The angiography after the operation showed that the majority of thrombosis were cleared and the blood of portal vein and superior mesenteric vein flowed smoothly.During the follow-up of 3 months to 3 years,all the patients’ status maintained well and no recurrence occurred.Conclusion Treatment of acute superior mesenteric vein thrombosis by percutaneous transhepatic portal vein thrombolysis is safe and effective.

The separation and analysis of hematoporphyrin photosensitizers PSD-001, photocarcinorin,photofrin II and other HPD preparations in comparison with authentic samples protoporphyrin (PP), 3-(8)-(1-hydroxyethyl) -8-(3-)-vinyldeuteroporphyrin ( HVD)and hematoporphyrin(HP) using thin layer chromatography(TLC) under different conditions are described here.It has been shown that there are 3 and 2 main spots in PSD-001 and photocarcinorin by common TLC, but 8-9 spots by high performance TLC.It has been found by comparison with authentic HP,HVD and PP that the amount of PP in PSD-001 or photocarcinorin is less than 3% and HVD,about 2.5% and 6.3% respectively,the HP content of photocarcinorin is much lower than other photosensitizers mentioned above and there is not any component near the origin spot.The Rf of one of the major components of photocarcinorin is 0.65.This component seems to be hardly found in other photosensitizers.These suggest that photocarcinorin is a new photosensitizing agent differing from HPD and photofrin II.

Objective: To study the effect of low concentration of ouabain MUM on intracellular calcium concentration ([Ca2+]i) in guinea pig ventricular myocytes and to understand whether low concentration of OUA can increase [Ca2+]i through Na+, K+-ATPase channel. Methods: The guinea pig ventricular myocytes were obtained by enzymatic digestion and the [Ca2+]i fluorescent density of individual myocytes was observed under confocal laser scanning microscope. The isolated ventricular myocytes were then incubated with different concentrations of ouabain(10-9, 10-8, 10-7, 10-6 mol · L-1). The sediment was subjected to Western blot analysis to assess the phosphorylation of Src by OUA. Results: In normal Tyrode' s solution and Ca2+-free Tyrode's solution, OUA (1 × 10-9 -1 × 10-6) mol · L-1 elevated [Ca2+]i in a concentration-dependent manner, with the elevation in normal Tyrode's solution more obvious (P < 0.05). Genistein (GST) (1, 10, 50, and 100 μmol - L-1) abolished the OUA-induced increases of [Ca2+]i in a concentration-dependent manner. There were two tyrosine-phosphorylated bands, with the molecular weights being 120 000 and 70 000. Compared with control group, the densities of the 2 bands in all OUA groups were significantly higher (P<0.05) and GST could obviously inhibit the elevating effect of OUA. Conclusion: Low concentration of OUA may promote opening of Ca2+ channel and release of intracellular Ca2+, and subsequently elevate intracellular free calcuim through phosphorylation of tyrosine and activition of OUA/Na+, k+-ATPase/Src signal transduction pathway.

Noninvasive continuous positive airway pressure (CPAP) for the ventilation treatment of obstructive sleep apnea-hypopnea syndrome (OSAHS) is the first-line treatment method. This article aims to describe the effect of surgical operation especially for the UPPP in the individualized comprehensive treatment of OSAHS and the importance of surgical technique. Lower compliance is the bottleneck of CPAP therapy in clinical application, for the OSAHS patients with treatment failure in CPAP or those cannot accept CPAP therapy, when with no other ideal instrument therapeutics, accurate diagnosis of position in airway obstruction with an adequate surgical operation is the treatment,of choice. Surgical operation is particularly important either as a fore-lying means to improve the CPAP treatment compliance or as an independence treatment method of OSAHS. The pharyngeal cavity is the most common obstructive plane in patients with OSAHS. The operation of traditional UPPP aiming at the expansion of pharyngeal cavity is the classics surgery to solve obstruction in this plane, the lower operation effective rate is the main reason of restriction in its development. How to improve the effective rate of surgical treatment of OSAHS is our surgical goal. The effective rate of surgical operation treatment in OSAHS rely on the following sides: to follow the OSAHS individualized comprehensive treatment principle, reasonable choice of surgical operation indication, the precise localization diagnosis of upper airway obstruction, adequate surgical operation and skilled surgical techniques.
Airway Obstruction ; Continuous Positive Airway Pressure ; Humans ; Palate ; surgery ; Pharynx ; surgery ; Sleep Apnea, Obstructive ; surgery ; Treatment Failure

BACKGROUND: This study investigated the effects of the intracoronary injection of nicorandil and tirofiban on myocardial perfusion and short-term prognosis in elderly patients with acute ST-segment elevation myocardial infarction (STEMI) after emergency percutaneous coronary intervention (PCI). METHODS: Seventy-eight STEMI patients with age >65 years who underwent emergency PCI were consecutively enrolled. These patients received conventional PCI and were randomly divided into a control group and a treatment group (n=39 per group). The control group received an intracoronary injection of tirofi ban followed by a maintenance infusion for 36 hours after surgery. The treatment group received intracoronary injection of tirofiban and nicorandil, and then intravenous infusion of tirofi ban and nicorandil 36 hours after surgery. The following parameters were measured: TIMI grade, corrected TIMI frame count (cTFC), TIMI myocardial perfusion grade (TMPG), STsegment resolution (STR) rate 2 hours post-operatively, resolution of ST-segment elevation (STR) at 2 hours postoperatively, peak level of serum CK-MB, left ventricular end diastolic diameter (LVEDD) and left ventricular ejection fraction (LVEF) at 7–10 days postoperatively, and major adverse cardiac events (MACEs) in-hospital and within 30 days post-operatively. RESULTS: Compared with the control group, more patients in the treatment group had TIMI 3 and TMPG 3, and STR after PCI was significantly higher. The treatment group also had significantly lower cTFC, lower infarction relative artery (IRA), lower peak CK-MB, and no refl ow ratio after PCI. The treatment group had signifi cantly higher LVEDD and LVEF but lower incidence of MACEs than the control group. CONCLUSION: The intracoronary injection of nicorandil combined with tirofi ban can effectively improve myocardial reperfusion in elderly STEMI patients after emergency PCI and improve shortterm prognoses.

BACKGROUND: Fiber posts are currently perceived as promising alternatives to cast metal posts, as their elastic modulus are similar to that of dentin, producing a favorable stress distribution and providing more esthetic outcomes for endodontically treated teeth. Failure of a fiber post and composite resin core often occurs at the junction between the two materials. This failure process requires better characterization.OBJECTIVE: To compare the bond strength of fiber posts after three surface treatments to a composite resin. METHODS: The coronal portions of 20 mandibular premolars with a single root canal were removed and were endodontically treated. After post space preparation, the roots were divided into four groups and the fiber posts and resin cores were lured. In the three testing groups, the surfaces of fiber posts were silaned, sandblasted or etched with hydrogen peroxide. In the control group, the surfaces of fiber posts were not treated. All specimens were exposed to cyclic loadings and thermal cycles. The roots were sectioned into slices with 1 mm thickness and their bond strengths were tested with a universal material testing machine. Test piece following testing was placed under optical microscope to observe the breakage method.RESULTS AND CONCLUSION: Stickiness strength was significantly greater in the sandblasted and hydrogen peroxide groups compared with the control group (P < 0.05). No significant difference in stickiness strength was determined between the sandblasted and hydrogen peroxide groups (P > 0.05). No significant difference in stickiness strength was detected between the silaned and control groups (P > 0.05). The splicing destruction among each group was the main breakage method between materials and fiber posts. The surface treatment of fiber posts with sandblast or hydrogen peroxide significantly enhanced the bond strength of the composite resin tested. The surface treatment of fiber posts with silane did not enhance the bond strength of the composite resin.

The essay discussed on how traditional Chinese medicine (TCM) recognizes the mechanism of fatigue stage from three organs of liver, spleen and heart. TCM believes the mechanism of fatigue in sub-health lies in stagnation of liver Qi due to emotional disorder and deficiency both Qi and blood due to overexertion hurting heart and spleen. Stagnation of liver Qi plays an leading role in the occurrence and development of fatigue state, while the strain of heart and spleen is the important manifestation of this state.

OBJECTIVE:To establish a RP-HPLC method for the determination of seratrodast concentration in human plasma.METHODS:The sample was determined on Lichrospher C18 column with the mobile phase consisted of 0.02 mol?L-1 potassium dihydrogen phosphate(containing 0.1% triethylamine,pH=5.0)-acetonitrile(70∶30)at a flow rate of 1.0 mL?min-1.The column temperature was 30℃ and the detective wavelength was set at 268 nm.RESULTS:The linear range of seratrodast was 28～5 600 ng?mL-1(r=0.999 5)with the lowest detection concentration at 28 ng?mL-1.The recovery rates of seratrodast at low,middle and high concentrations were 102.9%,100.4% and 99.7%,respectively;and both intra-day RSD and inter-day RSD were all less than 6%.CONCLUSION:The method developed in this study is applicable for the determination of plasma concentration and pharmacokinetic study of seratrodast.

Candida esophagitis is mainly caused by candida albicans. Risk factors include using antibiotics and glucocorticosteroid, chemotherapy and/or radiation therapy, malignancies and immunodeficiency syndromes. Acute onset of symptoms such as dysphagia and odynophagia is typical. It can coexist with heartburn, retrosternal discomfort, nausea and vomiting. Abdominal pain, anorexia, weight loss and even cough are present sometimes. Severe candida esophagitis may lead to development of strictures, hemorrhage, esophagotracheal fistula, and a consequent decrease in quality of life. The purpose of article is to review the epidemiology, pathogeny, risk factors, diagnosis and treatment of candida esophagitis.

Portal vein thrombosis (PVT) refers to thrombosis in the main portal vein and portal vein branches and is an important complication of liver cirrhosis.PVT can aggravate portal hypertension,increase the risk of bleeding,and lead to acute ischemic necrosis of the small intestine in case of shedding or retrograde movement.This article focuses on the new points of view on the mechanisms and treatment of PVT in cirrhotic patients and points out that treatment regimen should be selected based on patients'overall conditions.