1.Establishment of a sandwich ELISA method for measuring soluble IL-2 receptor
Chinese Journal of Immunology 1985;0(06):-
Two kinds of mouse monoclonal antibodies which recognize distinct epitopes of the IL-2 receptor (a chain) were used to establish a sandwich ELISA for measuring soluble IL-2 receptors. The results indicated that this method is sensitive and specific for detecting soluble IL-2 receptors of cell culture supernatant and serum from both healthy donor and patients with leukemia. Therefore this sandwich ELISA method is useful for basic and clinic immunology research.
2.Studies on the requirements for growth of Epstein-Barr Virus thransformed human lymphoblastoid B cells at low cell density
Chinese Journal of Immunology 1985;0(02):-
We obtained human lymphoblastoid B cells secreting human antibodies IgM and IgG to HFRS virus by Epstein-Barr virus(EBV)transfomation of PBMC.Conditioned media from the supernatant of a human fibroblast cell CRL1506 culture and irradiated CRL1506 cells as feeder cells could markedly promote the growth and antibody produotion of the human B lymphoblastoid cells at low cell density.It was shown that conditioned media contained a high level of interleukin 6(IL-6) .These results would be very useful for human monoclonal antibody technique including improving human B lymphoblastoid cell growth at low cell density and enhancing cloning efficiency andantibody production.
3.THE CHANGE IN VESTIBULAR FUNCTION IN PATIENTS WITH PARKINSON'S DISEASE AND ITS SIGNIFICANCE
An SUN ; Wenxuan CHEN ; Yongzh SUN
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
To study the changes in vestibular function in Parkinson's disease patients, and its significance, eletronystagmography (ENG) was used to examine 54 patients with Parkinson's disease, and also 20 hea thy elderly subjects. The results of vestibular function tests including sensory organization test, nystagmus test, cold caloric test and positional test of these two groups were recorded for statisticed analysis. It was found that the rate of vestibular dysfunction in Parkinson's disease patients were 81 4%, and that of the controls 30%. There was significant difference between the two groups ( P
4.Characteristics of some selected master dissertation topics in medical library and information science
Chen CHEN ; Yan SUN ; Jinli SUN
Chinese Journal of Medical Library and Information Science 2015;(5):35-37,65
The characteristics of master dissertation topics in medical library and information science and their se-lection methods were analyzed with the dissertations published since 2004 as the study object.The challenges and development trend in training of qualified personnel of medical library and information science were summarized , which may provide certain evidence for the training of masters in medical library and information science .
5.Variations of biomarkers in gastrointestinal stromal tumor and prognoses of the patients and targeted drug sensitivity
Journal of International Oncology 2012;39(4):295-298
Many researches show that the prognosis of gastrointestinal stromal tumor (GIST) are relatedtonumerousvariationsofbiomarkerssuchasnumericchromosomalaberrationsor nuclear/mitochondrialmicrosatelliteinstability.Inaddition, thechangesofproto-oncogeneand tumor-suppressor gene are found to influence the prognosis of GIST.The kit and platelet-derived growth factor receptor gene mutation are correlated with GIST progression,metastasis and targeted drug sensitivity,and then influence the prognosis of GIST.
6.The clinical analysis of levamlodipine besylate combined with benazepril in treatment of primary hypertension with left ventricular hypertrophy
Chinese Journal of Postgraduates of Medicine 2012;35(7):17-19
ObjectiveTo observe the effect of levamlodipine besylate combined with benazepril in treatment of primary hypertension with left ventricular hypertophy.MethodsNinety-eight primary hypertension with left ventricular hypertrophy patients were divided into two groups by random number table.Observation group(49 cases) was treated with levamlodipine besylate combined with benazepril,and control group (49 cases) was only treated with levamlodipine besylate.The clinical effect,the levels of systolic blood pressure (SBP),diastolic blood pressure (DBP),left ventricular posterior wall (LVPW),interventricular septal thickness (IVST),left ventricular end-diastolic dimension (LVEDD) and left ventricular mass index (LVMI) were compared between two groups.ResultsThe total effective rate in observation group was 93.9% ( 46/49 ),which was significantly higher than that in control group (77.6%,38/49 ) ( x2 =5.333,P <0.05).Before treatment,the differences were not statistically significant in the levels of SBP and DBP (P >0.05).After treatment,the levels of SBP,DBP,LVPW,IVST,LVEDD and LVMI were significantly decreased in both groups (P<0.05).Moreover,the levels of SBP,DBP,LVPW,IVST,LVEDD and LVMI in observation group after treatment were significantly lower than those in control group[ ( 121.27 ± 8.69 ) mmHg( 1 mm Hg =0.133 kPa) vs.( 131.54 ± 9.85 ) mm Hg,( 82.45 ± 4.69 ) mm Hg vs.( 88.49 ± 5.14 ) mmHg,(9.42 ± 1.58 ) mm vs.( 11.41 ± 1.92 ) mm,(9.56 ± 1.31 )mm vs.( 11.38 ± 1.85 ) mm,(47.86 ± 2.38 ) mm vs.(51.46 ±3.25) mm,(108.16 ±6.24) g/m2 vs.(119.21 ±7.68) g/m2] (P <0.05).Conclusion Levamlodipine besylate combined with benazepril in treatment of primary hypertension with left ventricular hypertrophy can improve the clinical effects and ease left ventricular hypertrophy status,which can be applied in clinic.
7.Tumor multidrug resistance and apoptosis evasion
Journal of International Oncology 2012;39(7):483-485
Most anticancer drugs inhibit tumor cell growth by inducing apoptosis.Apoptosis-evasion,also enhancement of anti-apoptosis ability(e.g.anti-apoptosis related genes over-expression or pro-apoptotic related genes loss,etc.)may lead to tumor cell multidrug resistance.Many researches show that apoptosis-evasion is one of the important factors resulting in tumor multidrug resistance.
8.Superficial siderosis of the center nervous system
International Journal of Cerebrovascular Diseases 2009;17(4):309-311
Superficial siderosis of the central nervous system is a seldom disease in clinical practice. Its most common clinical manifestations are hearing loss and ataxia. It is a hemosiderin deposition in the surface of the brain and spinal cord after chronic subarachnoid hemorrhage. Magnetic resonance imaging can make the diagnosis. However, further research and clinical verification are needed.
9.Effect of microsomal triglyceride transfer protein(MTP) gene polymorphism on angiopathy in type 2 diabetes
Chinese Journal of Diabetes 2006;14(2):98-100
Objective To investigate the relationship of MTP gene variation with type 2 diabetic angiopathy. Methods PCR and restriction-fragment-length polymorphism(PCR-RFLP) analysis and gene sequencing were used to investigate the influence of a common MTP gene polymorphism in promoter region on diabetic macroangiopathy. Results (1) A single nucleotide mutation of G→T was detected at 493 bp of upstream from the transcriptional start point.There was a significant difference in genotype frequency(χ2=9.162,P=0.01) and T allele frequency(χ2=10.322,P=0.001) between macroangiopathy group and non-macroangiopathy group.(2) MTP-493 TT group had the significantly higher serum levels of TC, TG, LDL-C, VLDL-C and lower HDL-C than other genotype groups.(3) Multivariate stepwise regression analysis showed that T allele, age, disease duration, diastolic blood pressure and total cholesterol were independent risk factors against type 2 diabetic macroangiopathy.The HDL-C level was negatively related with diabetic macroangiopathy. Conclusions MTP-493 TT genotype may be deeply involved in production of atherogenic dyslipidemia and associated with development of macroangiopathy in type 2 diabetic subjects.
10.Analysis on the community elder care in Bao'an district of Shenzhen
Chinese Journal of General Practitioners 2008;7(6):401-403
The status of health care for elderly in 110 community health service (CHS) centers of Bao'an District was surveyed.Data were collected from directors,health care managers and health workers in CHS centers by questionnaire.The results showed that the establishment of health file was satisfactory.but the management of health file needed to be strengthened,particularly for those required special care.The nutritional and psychological counseling should be specially focused.