The changes of PAF activity and the effects of anisodamine (654-2) were studied in the superior mesenteric artery occlusion (SMAO)shock in rabbits. The results showed that plasma PAF activity increased from 3.88?0.82 AU/ml before SM-AO to 9.71?1.26 AU/ml (P

Objective:To explore a scheduling method of rehabilitation medical resource for smart traditional Chinese medicine(TCM) for dysphagia because of cerebral apoplexy in order to save diagnosis time for patient and reasonably arrange treatment process for medical personnel.Methods: We designed the framework of smart TCM rehabilitation system, and proposed the medical resource scheduling model including acupuncture, massage and rehabilitation training. In addition, the genetic algorithm was employed to establish the scheduling method under the optimal objective towards the scheduling time.Results: (1) The treatment time of five dysphagia patients by using rehabilitation resource scheduling in Beijing Zhongguancun Hospital were saved 42.5% from the total treatment time compared to without scheduling; (2)The rehabilitation process of twenty virtual dysphagia patients were treated by the simulation scheduling, and 71% of total treatment time was saved. The efficiency of diagnosis and treatment was improved obviously .Conclusion: Smart TCM rehabilitation resource scheduling method can be used in an assisted rehabilitation therapy for dysphagia because of cerebral apoplexy, and it can improve the efficiency of diagnosis and treatment for patient and save a lot of medical resources.

Injections of propidium iedide (PI) into the lateral cerebral ventricle (LV) ofthe rat resulted in a prominent abnormality characterized by tremor,ataxia,andnystagmus.The intensity of PI fluorescence in the parenchyma of the brain fadedgradually away from the injection site and ventricles to the surfaces of the brain. In the forebrain it was seen that PI fluorescence reached the most lateral part ofthe ipsilateral caudate putamen nucleus.A constant neuronal labeling was observedin the septohippocampal nuclei,the A8-9-10 dopaminergic cell groups of themidbrain,the dorsal raphe nucleus,the median raphe nucleus,neurons within anddorsal to the medial lemniscus of the caudal midbrain,and Furkinje cells of thecerebellum.This neuronal labeling was bilateral.No distinct labeling was seen inother areas of the brain.Combined with Faglu histofluorescence,it was found thatalmost all of the dopaminergic neurons in the midbrain exhibited PI fluorescence.No labeled non-dopaminergic neuron was seen in A8-9-10.With a transection ofthe unilateral medial forebrain bundle,a prominent accumulation of PI fluorescencewas seen within the distal segments of catecholaminergic fibers near the transection,but no accumulation of PI was seen in the proximal segments.With LV injectionof Evans blue(EB)or DAPI or ethidium bromide,animals did not exhibit anyvisible abnormality.In animals with LV injection of EB or DAPI,although somelabeled cells were seen in the distant areas of the brain,their distribution wasdistinctly different from that of PI labeling.The above results indicate that besidesconfirming the LV injection of PI results in a prominent abnormality and PI isselectively uptaken by Purkinje cells,we have found that:a)PI is able to enter theparenchyma from the cerebrospinal fluid and diffuse widely in the brain;b)LVinjection of PI results in a selective labeling in certain specific areas of the brain,and those selectively labeled cells in A8-9-10 all are dopaminergic neurons;c)these dopaminergi(?) cells are labeled through axonal uptake and retrograde transportof PI.

The tanshinone ⅡA,an effective component of Radix Salviae Miltiorrhizae(RSM)can be extracted up to 90% in alcohol,but mostly decomposed in the later concentrating and drying procedures.Although reducing-pressure concentrating processes can reduce the decomposi- tion in experiment,but it can not preserve effectively the tanshinone during the large scale production because of the extended time of tanshinone in dampness and heat.Supercritical carbon dioxide extraction(SFE-CO_2)of RSM can give crystalline substance and dark red modifier when the alcohol is used as modifier at extraction pressure of 10 Mpa and the tem- perature at 40℃.The SFE-CO_2 technology can get higher concentration of tanshinone and be used during preparation production directly.The SFE-CO_2 technology is superior to the alco- hol-extraction technology.

To ensure the meridian can be measured and displayed correctly on the human body surface, a visualization method based on impedance and binocular vision is proposed. First of all, using alternating constant current source to inject current signal into the human skin surface, then according to the low impedance characteristics of meridian, the multi-channel detecting instrument detects voltage of each pair of electrodes, thereby obtaining the channel of the meridian location, through the serial port communication, data is transmitted to the host computer. Secondly, intrinsic and extrinsic parameters of cameras are obtained by Zhang's camera calibration method, and 3D information of meridian location is got by corner selection and matching of the optical target, and then transform coordinate of 3D information according to the binocular vision principle. Finally, using curve fitting and image fusion technology realizes the meridian visualization. The test results show that the system can realize real-time detection and accurate display of meridian.
Calibration ; Electric Impedance ; Electrodes ; Humans ; Meridians ; Vision, Binocular

Two-dimensional electrophoresis performed on a microfluidic chip could reduce the consumption of sample and reagents, realizing high-throughput analysis in a very short time. During the isoelectric focusing ( IEF) separation, the buffer for the two different separation methods should be kept from being mixed. After the IEF, the protein was transferred from the IEF channel into the second dimension channels. Based on the pseudo valve structure, this paper reported a method to deposit a titanium dioxide membrane at the interface of two dimensional channels, which enhanced the performance of the pseudo valve. The IEF electrophoresis and SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) procedures were characterized respectively. The total separation took 10-15 min, and the protein's migration rate was linear with the migration time, and inversely proportional to the logarithm of protein molecular weight. Based on the above results, the differential two-dimensional electrophoresis was performed to test the deviation between different measurements.

Objective:To evaluate the relationship between single nucleotide polymorphism (SNP) +45T→G in adiponectin gene exon 2 and type 2 diabetes mellitus (T2DM), obesity and insulin resistance (IR) in Han population in Guangxi. Methods:Polymer-ase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) was performed to identify the T-G polymorphism of adiponec-tin (exon 2) in 313 unrelated Han people from Guangxi including 95 normal glucose tolerance,103 impaired glucose tolerance and 115 type 2 diabetes mellitus. Results:In Han people of Guangxi, the SNP +45 GG genotype in adiponectin gene had significantly lower HDL-C levels than TT and TG genotypes in Han people(P<0. 05). The subjects with the GG genotype at position 45 had a signifi-cantly increased risk of IR (odds ratio of 2. 876[95%CI:1. 141-7. 248]) compared with those with the TT genotype at position 45. Conclusion:In Han people of Guangxi, the SNP +45 TT genotype in adiponectin gene has significantly higher HDL-C levels than GG genotype. The subjects with the GG genotype at position 45 have a significantly increased risk of IR compared with those with the TT genotype, while obesity and T2DM are independent on SNP+45 of adiponectin gene.

Objective To investigate the infection status of nanobacteria on patients with upper urinary calculi and healthy sub-jects ,and analyze the role of nanobacteria in the formation of upper urinary calculi .Methods The serum ,urine and calculus of 42 patients with upper urinary calculi were investigated by polymerase chain reaction (PCR) and by bacteria cultivation with 10% γ-FBS and PMBI 1640 .The resulting PCR products were sequenced for further comparison with the reported sequence in gene bank . The serum and urine from 30 healthy adults were used as controls .Results After 4 to 6 weeks′culture ,the white or yellow precipi-tate was visible at the bottom of the tube .The positive rate of PCR was 90 .4% in calculous patients urine and 6 .7% in healthy a-dults urine ,as the positive was 92 .8% and 6 .7% in serum .which there is significant difference (P<0 .01) .The positive rate of the nanabactria in urinary calculi was 95 .2% .The coincidence rate was 98 .72% between the PCR products and the reported sequence in gene bank .Conclusion Nanobacteria are widely existed in the serum ,urine and calculus of the patients with upper urinary calcu-li ,this indicate that the nanobacteria might be have the most important influence on the formation process of urinary calculi .

Zebrafish is widely used as a model organism in the process of drug discovery. It expresses drug metabolizing enzymes like cytochrome P450 (CYP450), uridine 5'-diphospho-glucuronosyltransferase (UGT) and nuclear receptors like pregnane X receptor (PXR), aryl hydrocarbon receptor (AHR), etc. This article summarized the profiles of main drug metabolizing enzymes and nuclear receptors, and reviewed the advances on xenobiotics metabolism in zebrafish.

Cytogenetics ; Humans ; Myelodysplastic Syndromes ; genetics