Cytogenetics ; Humans ; Myelodysplastic Syndromes ; genetics

To ensure the meridian can be measured and displayed correctly on the human body surface, a visualization method based on impedance and binocular vision is proposed. First of all, using alternating constant current source to inject current signal into the human skin surface, then according to the low impedance characteristics of meridian, the multi-channel detecting instrument detects voltage of each pair of electrodes, thereby obtaining the channel of the meridian location, through the serial port communication, data is transmitted to the host computer. Secondly, intrinsic and extrinsic parameters of cameras are obtained by Zhang's camera calibration method, and 3D information of meridian location is got by corner selection and matching of the optical target, and then transform coordinate of 3D information according to the binocular vision principle. Finally, using curve fitting and image fusion technology realizes the meridian visualization. The test results show that the system can realize real-time detection and accurate display of meridian.
Calibration ; Electric Impedance ; Electrodes ; Humans ; Meridians ; Vision, Binocular

0.05).Three groups all could improve total albuminuria(P

21 albino rats were used. The origin of the noradrenergic (NA) fibers in the septum was studied with a simultaneously combined retrograde fluorescent tracing and histofluorescence method. The results revealed that the NA neurons projecting to the septum were in A_1, A_2 and A_6 groups. The projections of A_1 were bilateral, But A_2 and A_6 projected dominantly to the ipsilateral septum, with a few to the contralateral. Retrograde labeled NA cells were localized in the rostrodorsal part of A_6 group, and scattered over the whole rostrocaudal length of A_(1,2) groups. No labeling was seen in A_4 and A_7 groups. In five of the animals, retrograde labeled NA cells were occasionally observed in A_5 group. In addition, some scattered labeled non-NA neurons were seen in the raphe and the ventrolateral reticular formation of the rostral medulla.

Two-dimensional electrophoresis performed on a microfluidic chip could reduce the consumption of sample and reagents, realizing high-throughput analysis in a very short time. During the isoelectric focusing ( IEF) separation, the buffer for the two different separation methods should be kept from being mixed. After the IEF, the protein was transferred from the IEF channel into the second dimension channels. Based on the pseudo valve structure, this paper reported a method to deposit a titanium dioxide membrane at the interface of two dimensional channels, which enhanced the performance of the pseudo valve. The IEF electrophoresis and SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) procedures were characterized respectively. The total separation took 10-15 min, and the protein's migration rate was linear with the migration time, and inversely proportional to the logarithm of protein molecular weight. Based on the above results, the differential two-dimensional electrophoresis was performed to test the deviation between different measurements.

OBJECTIVE:To improve package inserts of chemical drugs so as to promote safe,rational and effective use of chemical drugs.METHODS:A total of 558 package inserts of different chemical drugs commonly used in the clinic were collected from our hospital and other local hospitals and analyzed statistically based on the regulations stipulated by the "Norms of Package inserts of Drugs(trial)".RESULTS:Some important items such as pharmaco-toxicology,usage and dosage,pharmacokinetics etc in the package inserts of chemical drugs failed to meet the state standards,but the package inserts of the imported chemical drugs superior to those of the domestic ones,those of the injections superior to those of oral preparations,and those of oral preparations superior to those of external preparations.CONCLUSION:The package inserts of chemical drugs are far from perfect,which thus remain to be improved further.

OBJECTIVE:To investigate the drugs that labeled with the warning remarks for pregnant and lactant women for reference of patients' rational drug use.METHODS:The package inserts of drugs commonly used in our hospital were investigated and the drugs with warming remarks for pregnant and lactant women were collected.RESULTS:A total of 186 package inserts were found to have warning remarks for pregnant and lactant women.CONCLUSION:Pharmacists should attach great importance to the rational use of drugs for pregnant and lactant women in drug dispending and tighten control on the prescriptions with contraindication in prescription check,and they should cooperate with clinicians in the rational use and choice of drugs based on package inserts.

One hundred and forty mice, weighing 30-35 grams, were divided into irradiation group (12 Gy total body irradiation from a 60Co source), combined radiation-burn group (12 Gy total body irradiation and 15% TBSA third degree burns), and normal control group.The dynamic changes of the intestinal epithelium were observed with light and electron microscopes and autoradiography within 96 hours after injuries, and attention was paid to the formation and conversion of the misshapen cells. It was Jound that the proliferative cycle of the young cells in the intestinal crypts which were severely injuried by irradiation was significantly retarded, and their G1-phase to G2-phase were prolonged. These cells gradually changed into misshapen cells. Some of the misshapen cells could not divide and finally they wer.e desquamated; abortive proliferation was found in some of them; some others showed pathological karyokinesis. Thus polymorphic cells were formed. It was difficult to show that the misshapen cells could take part in the repair of the intestinal epithelium. It is speculated that the misshapen cells might to some extent have the function of absorption, secretion and barrier.

Some problems have influence on the quality of Clinical practice teaching of foreign students,including language communication barriers,teacher shortage and so on.To solve these problems,we worked hard on the rationale use of teachers,improvement of the teacher’s English level,giving lecture and collecting opinions to improve the quality of teaching.

Aim To explore the modulation of Salvia miltiorrhiza on hyperpolarization-activated current (Ih) channels in dorsal root ganglion (DRG) neurons of rats and identify the mechanism of Salvia miltiorrhiza in alleviating pain and inhibiting calcium overload. Methods The effect of Salvia miltiorrhiza injection on Ih channels in DRG neurons of rats were examined by using whole-cell patch clamp technique. Results The experimental results showed that the amplitude of Ih evoked by -150 mV was (-1.06±0.18) nA. The Ih could be fitted well into the single kinetics and the time constant of activation, τ was clearly voltage-dependent with τ=(322.14±28.81) ms at -100 mV, decreasing to τ=(62.51±9.78) ms at -150 mV. The reversal potential of Ih was (-35.03±1.12) mV measured from tail currents. But no significant differences were found between the DRG neurons in the absence and presence of Salvia miltiorrhiza injection (10%, 25%, 50%) in the current amplitude, the time constant of activation and the reversal potential. The only difference between the DRG neurons in the absence and presence of Salvia miltiorrhiza injection was the half-activation potential of Ih. In control recordings the half-activation potential was (-106.07±3.59) mV. By comparison, the half-activation potentials changed to (-111.59±3.79) mV (n=31 neurons, P＜0.05), (-119.37±4.96) mV (n=31 neurons, P＜0.05) and (-121.23±3.86) mV (n=31 neurons, P＜0.05) in the presence of 10%, 25%, 50% Salvia miltiorrhiza injection, respectively. Conclusion Only the half-activation potential of Ih in the arthritic and neuropathic rat models shifted in the depolarizing direction, which increased the electrophysiological activity of Ih and made it related to peripheral hyperalgesia. The selective inhibition of Salvia miltiorrhiza on the electrophysiological activity of Ih may be one of the mechanisms underlying its analgesic effects.