Child ; Diarrhea ; diagnosis ; therapy ; Humans

Objective To investigate the application of pathogenic examination and biomarkers detection of cerebrospinal fluid in diagnosis of intracranial infection .Methods Pathogenic and biomarkers examination were performed in cerebrospinal fluid of 49 cases with intracranial infection (infection group) and 49 cases of craniocerebral trauma but without intracranial infection (control group) .Results Adenosine deaminase activity obviously increased in tuberculous meningitis cases ,glucose levels decreased in bac‐terial meningitis and cryptococcal meningitis cases ,protein and IgA content increased in bacterial meningitis cases ,IgG levels in‐creased in bacterial meningitis and tuberculous meningitis cases ,IgM levels increased in tuberculous meningitis cases (P<0 .05) . There were no significant differences of biomarkers levels between viral meningitis cases and control group (P>0 .05) .Conclusion Combined pathogenic detection and biomarker detection of cerebrospinal fluid could be helpful for the diagnosis and treatment of intracranial infection .

In this study, a literature review was adopted to specify terminology of biosimilars and demonstrate the basic characteristic of biologics and relevant research and development ( R&D) procedures. The regulatory frame-work of the European Medicines Agency ( EMA ) guidelines on biosimilars was introduced. Explicitly, regulatory guidelines and scientific principles, regarding biosimilarity, safety and immunogenicity, extrapolation, labels and names, data protection, were systematically introduced, as well as interchangeability and pharmacovigilance, respec-tively. The purpose of the study is to provide regulatory references for Chinese legislators and recommendations on the R&D of biosimilars in the biopharmaceutical industry.

Objective To explore and analyze the effects of allicin prodrug on proliferation of esophageal cancer cell line Eca9706 and the expression of apoptosis gene.Methods Different concentrations of allicin prodrugs were divided into a total of four groups:A1 group(10μg/mL),A2 group(20 μg/mL),A3 group (40 μg/mL),A4 group (normal saline,0 μg/mL),and respectively applicated in esophageal carcinoma cell line Eca9706.After culturing for 1 days,2 days,3 days,cell proliferation was detected by MTT and expression of p53 at the mRNA level was detected by RT-PCR.Results The optimum concentration of proliferation inhibition of allicin prodrug on esophageal cancer cell line Eca9706 was 20μg/mL and the best inhibition time was 2 days;the gene level of esophageal cancer cell line Eca9706 was inhibited with a dose-dependent.Conclusion Allicin prodrugs could effectively inhibit the proliferation of esophageal cancer cell line Eca9706,and control the proliferation of esophageal cancer cells by regulating the expression of apoptosis associated genes,so as to eliminate tumor cells.

Ubiquitin-like proteins are structurally similar to ubiquitin. These proteins are processed, activated, conjugated, and re-leased from conjugates by enzymatic steps that are similar to the corresponding mechanisms for ubiquitin. Ubiquitin-like proteins regu-late a wide array of cellular processes through modification processes, such as nuclear-cytosolic transport, transcriptional regulation, protein stability, response to stress, and progression through the cell cycle. A large number of recent studies have found dysfunctional ubiquitin-like proteins in hepatocellular carcinoma. These proteins are important in tumorigenesis, cell proliferation, apoptosis, and an-giogenesis. Anticancer drug studies revealed that regulating protein modification by using ubiquitin-like proteins may alter the anti-tu-mor effects of chemotherapy and thus influence the chemosensitivity of hepatocellular carcinoma. Results indicate that ubiquitin-like proteins may become a new target for cancer therapy. The mechanism of ubiquitin-like proteins in tumorigenesis and hepatocellular car-cinoma progression is of great significance in the diagnosis and treatment of hepatocellular carcinoma.

A human diploid fibroblast cell line derived from normal abdominal skin of a 17-year-old female patient with ovary carcinoma was established and designated as HSF 79-2. The cultivation of skin fragments was employed in the primary explantation. The medium used was McCoy 5A supplemented with 15～25% calf serum. The grown fibroblasts were succesively subcultured at 1 to 2 split and stopped at the 45th to 54th passage.Having reached confluence the cells still had the ability to divide during 16 months observation if the medium was changed periodically. They displayed the property of dense growth and forming heavy, multilayer sheets, which became a visible membrane to the naked eyes. Under histological examination the membrane had the appearance of connective tissue. Part of the cells were subcultured again after 3,6, and 12 months maintenance in the same culture flask. Their growth character, ploidy, and the generation time were similar to that of the cells passaged normally.The characteristics of this cell line mentioned above appear to be shared by other diploid fibroblasts. It might be used for preserving cell lines and as a model for studying cell motility and differentiation.

Objective To investigate the effects of L-thyroxine(LT4) on serum lipid and bone metabolism in aged patients with subclinical hypothyroidism (SH). Methods Seventy-eight patients with diagnosed SH were randomly assigned to receive LT4 (therapy group) or placebo(control group) for one year. Mean dose for LT4 replacement was (80.1?37.2) ?g/d. The changes of clinical symptoms, body mass index (BMI) and serum levels of total cholesterol(TC), triglycerides(TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol(LDL-C), apolipoprotein A 1(ApoA 1), apolipoprotein B 100 (ApoB 100 ), calcium ion(Ca 2+ ), phosphorus ion (P 3+ ) and alkaline phosphatase (AKP) and bone mineral density (BMD) were measured before and after therapy. Results After LT4 therapy, clinical symptoms were ameliorated. BMI and serum TC, TG, LDL-C and ApoB 100 levels were significantly reduced 〔(24.6?3.1 vs 23.3?2.2),(5.65?1.42 vs 4.43?0.81),(1.81?0.66 vs 1.43?0.40),(3.64?0.84 vs 2.68?0.63)mmol/L,(0.98?0.22 vs 0.83?0.21)g/L,P

With the gradual standardization in the medical management of family beds, widespread attention has been given to the establishment of complete medical records for patients using family beds. To achieve genuine standardization, a set of new nursing records for family beds were designed in light of the features of family nursing. This set of records attaches importance to the assessment of the patients' daily life skills, family resources, and living environment. It is easy to use and gives expression to the independent and scientific nature of community nursing. The designed protocol for family nursing plays some role in safeguarding the interests of both physicians and patients.

OBJECTIVE:To explore the way of establishing the ADR victims'relief system in our country.METHODS:To introduce the ADR victims'relief system of Japan,analyse the significance of establishment and practice of ADR victims'relief system in China.RESULTS&CONCLUSIONS:The ADR victims'relief system of our country should be established as soon as possible so as to guarantee the victims'rights.

OBJECTIVE:To explore the reason for the "tragedy of anti-commons" in biomedical patent and its effect on the development of biomedical industry.METHODS:The mathematical models of "commons" and "anti-commons" gambling were established based on the analysis on emergence of "patent bush" resulted from the abuse of biomedical patents,and the profit conflict of the patents power related to elementary researcher and applicant researcher was analyzed.RESULTS:The potential "tragedy of anti-commons" affects the enthusiasm of the biomedical researchers,hinders the rapid development of biomedical industry and the improvement of technological level.CONCLUSIONS:The corresponding measures should be taken from aspects such as system,research model,and patent applicant model to balance the possessiveness and exclusiveness,avoid the "tragedy of anti-commons" and facilitate the development of biomedical industry.