Objective To investigate the antithrombotic effects of polydatin.Methods Injection of arachidonic acid into mouse,electrically stimulated carotid artery and inferior vein ligation in rats were used to evaluate polydatins antithrombotic effects respectively.Results Polydatin presented obvious antithrombotic effects in three thrombotic models and showed a good dose-effect relationship.Conclusion It is suggested that polydatin has evident antithrombotic effects in artery,vein and micro-circulation.

Chronic myeloid leukemia (CML) is a malignant clonal proliferative disease,which originates from multifunctional hematopoietic stem cells of bone marrow.Imatinib is used as the first-line treatment for CML currently,owing to its obvious curative effect for patients in chronic phase and survival time prolonged significantly for patients in accelerated phase and blast crisis.With the long-term application of imatinib in clinic,the drug resistance and intolerance gradually appear,resulting in decreased long-term effects of CML.This paper will review the current status of the pathogenesis and treatment of CML.

The factors influencing public access to life science information were analyzed from its scope, cost, chan-nels, mechanisms and laws with suggestions put forward for the access to life science information without any barrier.

Objective To investigate the changes of retinal ganglion cells (RGCs) exposed to hypoxia and the mechanism. Methods RGCs were isolated from the retina of neonatal Long Evans rats aged 1 day and cultured, then divided into normal control group and hypoxia group (cultured in incubator containing 1 O_2, 5 CO_2 and 94 N_2). At 1, 3, 12 and 24 h after incubation, the calcium ion level by laser scanning confocal fluorescence microimaging system, the activity of SOD and the content of MDA by biochemistry technology, TNF-? by ELISA were detected. Results No changes of calcium ion level in RGCs were observed in normal control group. The calcium ion level increased significantly in the hypoxia group (P

Objective To investigate the changes of systolic pressure,diastolic pressure,oculocardiac reflex(OCR) and vision of patients suffering from cardiovascular disease when they applied phacoemulsification.Methods Totally 168 cases(182 eyes) who had underwent phacoemulsification were analyzed in random selection.Of all the cases,97(106 eyes) were combined with cardiovascular disease,the others were without any general disease.The changes of blood pressure,OCR,ECG and vision were observed in two groups.Results Compared with preoperative period,the mean systolic blood pressure increased significantly during operation,but the differences of diastolic pressure,OCR,ECG and vision were not significant between the two groups.Conclusion The safety and reliability of the surgery depend on reasonable medication,careful surgical procedure and monitoring for the patients with cardiovascular disease in the peri-operation period.

Objective To establish clonal human embryonic stem cell lines and investigate their biological characteristics. Methods Cells were derived from one inner cell mass of human blastocyst, multiplied for 20 passages, and then dissociated into single cell suspension by digestion with 0.5% trypsin. Single cell was picked up and plated into individual well of a 96-well plate containing feeder-layers directly under a dissection microscope. The outgrowth clones were passed by treatment with collagenase. Surface markers were detected by cytochemistry and histoimmunochemistry. Karyotypes were tested using standard G-banding techniques. The pluripotency was analyzed by inoculating cells into severe combined immunodeficient (SCID) mice. Results Two clonal human embryonic stem cell lines were established. Cells of these two lines possess the characteristics and differentiating potencies: normal 46 XX karyotypes; expressing a series of surface markers such as: alkaline phosphotase, stage-specific embryonic antigen (SSEA)-4, tumor recognition antigen (TRA)-1-60, TRA-1-81 etc; and forming teratomas comprising derivatives of three embryonic germ layers such as neural tissue, cartilage, squamous epithelium and columlar epithelium when injected into SCID mice. Conclusions The two single cell-cloned human embryonic stem (hES) cell lines were derived in our laboratory. The cells possess stable biological characteristics of undifferentiated hES cells.

Objective To investigate the expressions of enhancer of zeste(EZH2) in carcinoma of esophagus and its potential significance. Methods The expression and distribution of EZH2 were determined on the esophageal squamous carcinoma,adenocarcinoma by using immunohistochemistry.We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.Results The immunohistochemical staining showed EZH2 protein was highly expressed in esophageal squamous carcinoma and adenocarcinoma tissues.The positive staining was observed in the nuclear region.Normal tissues and corresponding paracarcinomatous tissues were stained weakly.Western blotting analysis showed that the strongest positive signals were detected in the esophageal squamous carcinoma(P

OBJECTIVE To investigate the drug resistance status of Klebsiella pneumoniae,Escherichia coli and(Enterobacter cloacae) for reasonable use of antibiotics. METHODS After genus identification,drug resistance profile was analyzed by K-B method. RESULTS The predominant isolates from Enterobacteriaceae were(K.penumoniae) and E.coli.The resistant rate of the three commonly encountered bacteria in Enterobacteriaceae to antibiotics ascended yearly,with increasing isolates harboring ESBLs from 16.5%(2000) to 32.4%(2004). CONCLUSIONS Rational use of antibiotics should be obey the results of laboratory antibiotics susceptibility test to control the increasing trend and forbid outbreak of resistance.

OBJECTIVE To investigate drug resistance status of common bacteria to ciprofloxacin in hospital for reasonable use of antibiotics.METHODS After genus identification,drug resistance profile was analyzed by K-B method.RESULTS The resistant rate of common bacteria to ciprofloxacin ascended yearly from 40.8%(2000) to(51.7%)(2004),which predominated with Escherichia coli,coagulase negative Staphylococcus and S.aureus,respectively.CONCLUSIONS Rational use of fluoroquinolones should be obey the results of laboratory antibiotics suceptibility test to control the increasing trend of resistance.

0.05).There were significant difference but 50mA image quality betweer the rest of the three groups(P