Transient ischemic attack (TIA) is an independent risk factor of stroke. About two-thirds patients with TIA will reoccur TIA or stroke. To predict the risk of stroke after the onset of TIA may identify the prognosis of patients, so as to have an intervention for the high-risk patients as early as possible. This article reviews the various risk assessment tools for stroke after TIA, in order to provide ideas for the prognostic assessment of TIA.

Aim To develop an HPLC method for the determination of Aconitum alkaloids extracted from Radix aconiti preparata in rats. Methods Waters 2690@996 PAD system was used. The analytical column was a Halsil 100 C18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase was water, methanol and diethyl amine at the ratio of 75∶ 25∶ 0.1. The flow rate was 0.9 mL·min -1. The wavelength of the detector was 240 nm. Results The linear ranges of aconitine in the heart, spleen, lung and kidney were 0.4-100 μg·mL -1, the correlation coefficients were 0.997 2, 0.998 6, 0.999 3 and 0.999 4, respectively. The linear range of aconitine in liver was 2-200 μg·mL -1 and the correlation coefficient was 0.999 0. The linear ranges of hypaconitine in heart, liver, spleen, lung, kidney, brain and spinal cord were 5-100 μg·mL -1, the correlation coefficients were 0.999 4, 0.999 7, 0.999 8, 0.998 4, 0.999 8, 0.999 8 and 0.999 7, respectively. Detection limits (S/N=3) of aconitine and hypaconitine were 0.4 μg·mL -1. The recoveries of aconitine and hypaconitine ranged from 88.7% to 102.2% and 86.5% to 101.3%, respectively, and the RSD of precision of aconitine and hypaconitine was 10%. Conclusion It appears to be an accurate and effective method that can offer reference basis for in toxication of Radix aconiti preparata clinically.

The paper covered key progress of the management system reform for China's public hospitals and analyzed main roadblocks in the reform.It proposed such policy recommendations as clearly separating the regulation and management affairs,concentrating management authorities to build an integrated management system,establishing a clear-cut appraisal criteria for accountability,and completing supportive reforms for effective management.

Pancreatic cancer is a kind of high degree of malignant gastrointestinal tumor,and its incidence has increased significantly in recent years.Due to the low early diagnosis rate,and the extreme low middile or late resection rate of pancreatic cancer,Pancreatic cancer's prognosis is poor.Therefore,improving the early diagnostic rate of pancreatic cancer has very important significance for improving the prognosis.Early diagnosis of pancreatic cancer is dependent on tumor markers detection to a great extent.And proteomics progress rapidly in recent years and has been widely applied in clinical research.It provides an effective way to find markers.Among all of the spec imens,secrum is easy to obtain,and it allows repetitive detection.What is more,secmm is also renewable.So it is the most ideal for biological specimens.In this review,the proteomics technology role in pancreatic cancer patients serum detection were summarized.

The study aims to identify related substances in orlistat pharmaceutical substance from different production technologies. Advanced technologies including UPLC-QTOF-MS and UPLC-QTrap-MS were used and the structures or molecules of 10 kinds of related substances in orlistat were identified. The experimental data were valuable to the quality control and development of orlistat.

Objective To study the stress resistance of Rhodiola under freezing injury stress. Methods With R.kirilowii, R.crenulata, R.Algida and R.fastigiata as the experimental materials, the effects of freezing injury stress on emerging time, duration of seedling emergence, emergence rate, surviving rate, the activity of SOD and POD were observed by field test and experimental detection. Results Before seeding, the effect of freezing injury stress on R.kirilowii was the largest, and that on R.fastigiata was the least. When injury stress happened during seedling stage, surviving rate, increase of SOD and POD activities of R.kirilowii was the least, indicating the freezing injury was severe. R.fastigiata was the best in those indexes. The stress resistance of Rhodiola was as follows:R.fastigiata > R.crenulata > R.Algida > R.kirilowii. Conclusion R. fastigiata and R.crenulata are good choice in introduction and cultivation of Rhodiola in Gannan area of Gansu province, and R.fastigiata is the best.

Objective To investigate the effect of adenoviral-mediated interleukin-10 (IL-10) gene transfection on systemic inflammatory responses in a rat model of ventilator-induced lung injury. Methods Thirty adult male SD rats weighing 200-220 g were randomly divided into 5 groups (n=6 each): group Ⅰ control (group C); group Ⅱ adenovirus without IL-IO gene + 2 h mechanical ventilation (MV) (group B, ); group II1 adenoviruswithout IL-IO gene + 4 h MV (group B_2); group Ⅳ adenovirus with IL-10 gene + 2 h MV (group A_1) and group Ⅴ adenovirus with IL-10 gene + 4 h MV (group A_2). In group Ⅱ-Ⅴ the animals were mechanically ventilated (airway pressure = 25 cm H_2 O) and adenovirus was injected into right ventricle at 48 h before MV. Blood samples were taken from abdominal aorta at the end of 2 h or 4 h MV for determination of serum IL-8 and IL-10 concentrations. The animals were then killed. The pulmonary specimens were obtained for examination of ultrastructure with light and electron microscope. Results Mechanical ventilation significantly increased serum IL-8 and IL-IO concentrations in a duration-dependent manner in group Ⅱ-Ⅴ as compared with group C. Trans-pulmonary administration of adenoviraI-mediated interleukin-10 gene significantly decreased serum IL-8 concentration and increased serum IL-10 concentration and reduced ventilator-induced lung injury alter MV in group A_1 and A_2 as compared with group B_1 and B_2. Conclusion Adenoviml-madiated IL-10 gene transfection can attenuate the systemic inflammatory response and reduce ventilator-induced lung injury.

Objective To investigate the predictive value of ADC value in grading of neuroepithelial tumors. Methods The clinical data and images of 70 patients with neuroepithelial tumors pathologically proven were collected and analyzed restrospectively. All the patients were classified into low(WHOⅠ or Ⅱ)and high(WHO Ⅲ or Ⅳ)grade groups which included 40 and 30 cases respectively accroding to the 2007 WHO classification of tumours of the central nervous system. All the patients underwent plain and contrast-enhanced MR scan and DWI before surgery. The minimum ADC(MinADC)value was measured postoperatively on ADC maps. The Ki-67 labeling index(Ki-67 LI)of tumor tissue was determined by immunohistochemistry.MinADC values for two groups were analyzed using student t test, while the age and Ki-67 LI for the two groups was analyzed using Mann-Whitney test(P＜0.05 considered to be significant＝.Also the MinADC valHe and Ki-67 LI were analyzed with Pearson correlation. The receiver operating characteristic analysis was used for evaluation for grading neuroepithelial tumors of the patients using MinADC. Results The mean MinADC value[(1.08±0.31)×10~(-3) mm~2/s]of the low grade group was significantly higher than that[(0.74±0.18)×10~(-3) mm~2/s]of the high grade group(t=5.42,P＜0.05＝,and the Ki-67 LI of the low grade group[0-50%,median 4%]was significantly lower than that[0-75%,median 25%]of the high grade group(U=325.50,P＜0.05＝.There was a negative correlation between MinADC value and Ki-67 LI(r=-0.30,P＜0.05＝.The area under the ROC curve was 0.85,and the cutoff MinADC value of 0.86×10~(-3) mm~2/s for the differentiation between high and low grade neuroepithelial tumors provided the best combination of sensitivity(90.0%)and specificity(77.5%)(receiver operatingcharacteristic analysis).Conclusion MinADC value is helpful for prediction of neuroepithelial tumor grade.

Objective To analyze the causes and possible mechanisms of vertigo for emergency,and to discuss the diagnosis and therapy methods.Furthermore, the possible effective preventions were suggested.Methods 86 elderly patients with vertigo for emergency were enrolled to analyze the causes through symptoms,signs and accessory examinations.Results The majority of the patients fell within the age range between 60～69 ,it occurred more frequently in female patients than in male.The main causes were cervical vertebra disease,hypertension atherosclerosis,acute cerebrovascular disease,hyperlipidemia,diabetes mellitus and autonomous nerve function disorder.Conclusion The mechanisms which induce vertigo in elderly patients are concerned with disturbance of vertebra-basal and blood supply system.It is not only resulted from single disease,but also from several factors.Moreover,it often co-exist with multi-diseases, which shows that symptomatic treatment is still the essential method for curing vertigo in elderly patients for emergency.

Dysregulation and activation of immune processes are important in age-related macular degeneration (AMD) pathogenesis.The single nucleotide polymorphism of complement factor H is widely recognized as a risk factor to AMD.Over-activation of nod-like receptor3 and polymorphism of Toll-Like Receptor 3 also associated with AMD.Except for innate immune processes,adaptive immunity also play a critical role in AMD,a growing body of evidence supports that auto-antibodies and T cells are related with AMD.Additionally A2E and lipid oxidation byproducts might also have a role in AMD pathogenesis.