The present study was to investigate whether peripheral NMDA receptors were involved in the persistent nociceptioninduced by subcutaneous (s. C. ) bee venom injection in the conscious rat by using quatitative pain scoring methods, a.c. Bee venom injection into one hindpaw resulted in a persistent, monophasic nociceptive response characterized by continuously flinching.lifting and licking the injected paw for more than I h. The non-competitive. NMDA receptor channel blockers, ketamine and ME-801, were administered s.c. 5 or 20 min after bee venom. Local ketamine injection produced a suppression of flinching reflex by 20. 90±2.88% and 45.76±13.9%, while that of lifting/bcking time by 39. 53±10. 05% and 59.94±5.53%, at doses of 25mmol/L and 50 mmol/L respectively without any motor disturbance, Local MK-801 resulted in an inhibition of flinching reflexby 22.84±3.12% and 49.53±5.35%. While that of lifting/licking time by 17. 49±5.67%and 53.49±3. 87%. At doses of 10μmol/L and 100 ,μmol/L respectively also with no motor disturbance. However, s. C. Administration of ketamine and MK-801 inior region symmetrical to the bee venom injection site on the contralateral hindpaw produced no change in the nociceptive behaviors, suggesting that the analgesic actions of keramme and MK-8Ol were not the result of systemic effects. The present resultsuggests that peripheral NMDA receptors are involved in the production of persistent pain.

BACKGROUND: Serum levels of high-sensitivity C-reactive protein (hs-CRP) can be used to predict cardiovascular injury.OBJECTIVE: To investigate the serum levels of hs-CRP in a rabbit model of atherosclerosis following repeated long-termexposure to positive acceleration and to predict the risk of cardiovascular injury.METHODS: Twenty-four New Zealand purebred rabbits were randomly divided into a positive acceleration group and a controlgroup. Rabbits in the positive acceleration group were exposed to +4 g rotation for 20 seconds with an acceleration of 1 g/s. Therotation interval was 5 minutes and totally three rotations were daily performed for 3 days per week. 0.5 g rotation was addedevery week, and by week 4, +6 g rotation was added, and the exposure lasted for 40 seconds.RESULTS AND CONCLUSION: Exposure to positive acceleration yielded significant effects on serum level of hs-CRP in rabbitswith atherosclerosis compared with before exposure (P < 0.01), but exposure time did not produce effects on serum level ofhs-CRP (P > 0.05). The interaction between exposure to positive acceleration and exposure time also did not produce significanteffects on serum level of hs-CRP (P > 0.05). Hyperplastic foam cells in the aortic tunica intima and the superficial layer of mediaincreased with the prolongation of exposure to positive acceleration. Repeated long-term exposure to positive acceleration maylead to a long-term high serum level of hs-CRP in rabbits.

0.05),but all the outcome measures at 1 month in the treatment group were significantly improved as compared with control group(P0.05).The mean light sensitivity in the treatment group was better than in control group(P

Objective To investigate the correlation between indexes of thrombelastogram (TEG) and hemorrhagic transformation (HT) after acute ischemic stroke.Methods The cases of acute ischemic stroke but not receiving early reperfusion therapy were recruited in our hospital from January to November 2016,and were divided into HT group and non-HT group.Their general clinical characteristics and TEG indexes were retrospectively analyzed,and comparison between two groups and multivariate Logistic regression analysis were performed.Results A total of 71 cases were enrolled and 11 of them were divided into HT group.The percentages of massive cerebral infarction and cardio-embolic stroke were significantly higher in HT group than those in non-HT group (P<0.05),whereas the levels of CI in HT group were significantly lower than thoes in non-HT group(P<0.05).Multivariate Logistic regression analysis showed that massive cerebral infarction (OR=13.172,95%CI:1.414-122.671) and CI(OR=0.554,95%CI:0.321-0.956) were independently correlated with HT(P<0.05).Conclusion CI is independently correlated with HT after acute ischemic stroke,and may be a potential predictor of HT.

Three Modules and Three Stages proposed by guidelines on college English teaching that will be issued by the Ministry of Education gives direction for college English teaching.Through the investigation of the students' cognition and learning of English curriculum,combined with the teaching experiment,the new system of English curriculum,which reflects the characteristics of medical colleges and universities,has been preliminarily established,aiming at cultivating medical students' comprehensive English abilities in medical study and career development.Considering students' language abilities in different stages,college English curriculum can be designed in three modules,that is,English for general purpose (EGP),English for academic purpose (EAP) and intercultural communication,in three stages,that is,the basic stage,the improvement stage,and the development stage.The practice shows that the new curriculum system can promote the interaction,reform and development of teachers,teaching teams and Foreign Language Department.

Objective:To evaluate the feasibility of the 3-D surface reconstruction by multislice spiral CT(MSCT) technique in the diagnosis of impacted teeth in maxilla.Methods:Eighty two cases with impacted teeth in maxilla were examined with volumetric CT scan using MSCT,the dental reconstruction images were obtained with multiplanar reformation technique and 3D-reconstruction technique.Results:Dental surface 3D-reconstruction image could display the shape,location and eruption orientation of the impacted teeth clearly.Conclusion:3-D surface reconstruction by MSCT can provide valuable diagnostic and anatomic information of the impacted teeth in maxilla.

Objective To detect the antimutagenesis of carrot juice and to provide necessary data for the development of fruit-vegetable beverage. Methods Revised rat salmonella mutagenicity test was used, strains had been cultured for 48 hours at a constant temperature of 37 ℃. We counted the anaphysis strains produced in the paper circle, and judged whether there was a antimutagenesis effect according to the difference of the anaphysis strain number between the samples and the dissolvent controls. We also judged the mutagensis effect of the samples based on the anaphysis strains outside the paper circle. Results TA98-TA100 strains were all inside the normal scope, mutagenesis effect of all samples had not been observed. Conclusion Under our laboratory conditions, the inhibition rate of sample Ⅰ,Ⅳ on the induction of N-methy1-N/-nitroso-N-nitrosoguanidine(MNNG) to TA100-S9 and of sample Ⅱ, Ⅲ on the induction of aflatoxin B1( AFB1) to TA+S9 was high, and dose-response effect was obvious, also the inhibition effect of both sample Ⅰ,Ⅱ on the induction of AFB1 and that of sample Ⅰ on the Benzo(a)pyrene (BAP) to TA100+S9 was relatively high, but there was no dose-response effect exist.

OBJECTIVE:The effects of zinc fructose diphosphate(ZnFDP)in different concentrations on the growth of cultured newborn mouse cerebral cortex neurons were observed METHODS:The newborn mice cerebral cortex neurons were cultured and different dosages of ZnFDP were added with final concentrations of 2 5?g/ml,12 5?g/ml and 125?g/ml The convert phase microscope was used to observe the growth of dendrites and cell bodies of neurons The survival neuron count and LDH assay were carried out to investigate the effect of ZnFDP on the growth and development of neurons in different culture periods RESULTS:After 48h,the number and the length of neural dendrites in 12 5?g/ml ZnFDP group were increased but the maximum diameter of cell bodies of neurons showed no change There were no significant differences in all parameters observed between 2 5?g/ml group and control group,while 125?g/ml ZnFDP obviously inhibited the differentiation of neurons The survival neurons on 12 5?g/ml ZnFDP group outnumbered those in the control group after 3d,7d and 10d culture,and the LDH activity in 12 5?g/ml ZnFDP group was lower than that in control group after 7d and 10d culture CONCLUSION:This study suggests that a suitable dose of ZnFDP can promote the growth and development of cerebral cortex neurons

Exertional heat stroke (EHS) is an emergency with a high mortality rate, characterized by acute onset and identification difficulties. EHS prevention focuses on evaluating the environment by professionals, making preventive measures in advance, identifying internal and external risk factors for the onset of disease, carrying out prior heat adaptation and endurance training, monitoring the status of high risk persons in real time. After occurrence of EHS, the key to success treatment is on-site accurate identification and diagnosis and rapid implementation of effective cooling measures, thus winning time of EHS patients transferred to the hospital for the treatment. This article reviews the current status of EHS prevention and treatment in domestic and foreign from the scene to the hospital. Summarizing the recognition, diagnosis, cooling measures, treatment concepts and principles of EHS, we hope to provide a reference for the rescue of EHS in hospital and outside.