Objective To find a grading method for assisting in evaluating hypoxia degree of patients with pulmonary heart disease non-invasively. Methods To design a quantitative method to score degree of hypoxia of 67 patients with pulmonary heart disease by using acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) as a frame and to make comparative analysis between the grading result and artery blood gas to find the correlation. Results There was a negative correlation between evaluation mark and pH、PaO2、SaO2 and a positive correlation between the evaluation mark and PaCO2, according to statistics. Conclusions Although the grading table of hypoxia evaluation can not substitute blood gas analysis, acting as a non-invasive method to assist in evaluating the hypoxia degree of patients with pulmonary heart disease, it can not only relieve patients' repeated punctuation pain, but also achieve the goal of estimating hypoxia.Consequently the non-invasive method to evaluate hypoxia is of clinical value.

Objective To study the expression of carbonic anhydrase 9(CA9) in clear cell renal carcinoma(ccRCC), and the relationship between the expression and recurrence .Methods Paraffin-embedded tissues from 53 ccRCC cases were collected during the past 3 years,and immunohistochemistry was used to detect the expression of CA 9.Fifty-three cases were followed up to evaluate the disease-free survival ( DFS) and recurrence .Association of CA9 expression with recurrence was then analyzed .Results Localized RCC recurred in eleven cases , the median DFS was 10 months, the integral optical density ( IOD) of the recurrent cases was 230 879 .84 ±64 878 .72 while the IOD of the thirteen nonrecurrent cases was much higher,444 993.31 ±146 946.27(P<0.01).Conclusion There might be a positive correlation between the low expression of CA9 and the recurrence of ccRCC .CA9 may help predict the ccRCC patients ’ prognosis.

OBJECTIVE:To determine the content of 5 kinds of heavy metal elements in Astragalus membranaceus from Longxi of Gansu province,i.e. Pb,Cd,Cu,As,Hg. METHODS:Wet digestion pretreatment was carried out for samples and atomic absorption spectrometry (AAS) was used for the determination of heavy metal elements. RESULTS:The contents of 5 kinds of heavy metal elements in A. membranaceus from Longxi of Gansu Province were lower than the limit. CONCLUSION:AAS method can accurately determine the content of heavy metal elements in A. membranaceus.

Objective To investigate the levels of peripheral blood plasma M-ficolin/L-ficolin and their correlation with clinical indexes in patients with rheumatoid arthritis (RA).Methods M-ficolin/L-ficolin was detected in the plasma of 61 RA patients who were grouped according to the disease activity and 18 healthy controls by enzyme-linked immunosorbent assay (ELISA).The relationships between the levels of M-ficolin/L-ficolin and laboratory parameters,disease activity were investigated in RA patients.Statistical analysis were performed by t-test,one-way analysis of variance (ANOVA),rank-sum test,correlation analysis was carried out with Pearson/ Spearman rank correlation test.Results ① M-ficolin levels in 61 RA patients were [M (QR) 39 (24) ng/ml] significantly higher than healthy controls (14±4) ng/ml (Z=-5.72,P＜0.05).L-ficolin levels in 61 RA patients were (1.7±0.6) μg/ml obviously higher than healthy controls (1.3±0.5) μg/ml (t=2.55,P=0.013).② The M-ficolin levels of 61 RA patients in low,mediate,high disease activity group were (21±5) ng/ml,[M(QR) 35(13) ng/ml),[M(QR) 50(25) ng/ml].There was significant difference among the three groups (P＜0.01).The L-ficolin levels of 61 RA patients in low、mediate,high disease activity group were (1.2±0.5) μg/ml,(1.7±0.4) μg/ml and (2.2±0.6) μg/ml.There was significant difference among the three groups (P＜0.05).(M-ficolin levels were positively correlated with disease activity score (DAS)28,erythrocyte sedimentation rate (ESR),C reactive protein (CRP),rheumatoid factor (RF),white blood cell (WBC),Note Express User Tool (NEUT),MONO,blood platelet (PLT) (r=0.830,0.692,0.725,0.667,0.388,0.397,0.338,0.476,P＜0.05),L-ficolin levels were positively correlated with DAS28,ESR,CRP,RF,WBC,NEUT,MONO,PLT (r=0.653,0.593,0.630,0.468,0.254,0.302,0.312,0.340,P＜0.05).Conclusion M-ficolin/L-ficolin levels increase in RA patients and correlate with disease activity,which suggests that the complement system may play an important role in the pathogenesis of rheumatoid arthritis.

Objective: To investigate the role of host derived matrix metalloproteinases (MMPs) in the pathogenesis of pulpitis. Methods:The expressions of MMP-2 and MMP-9 were examined by immunohistochemical method and the activity of MMP-2 and MMP-9 were measured by gelatin zymography in 20 clinically healthy and 20 inflamed human dental pulps of extracted mandibular 3rd molars.Results:In clinically healthy and inflamed human pulps both MMP-2 and MMP-9 were expressed in odontoblasts and fibroblasts. The pro-MMP-9 was detectable in both inflamed and healthy pulps. The level of pro-MMP-9 was higher in inflamed pulps than that in the healthy(P0.05).Pro-MMP-2 and active MMP-2 were not detectable in the pulps of the two groups. Conclusion:MMP-9 may involve in the pathogenesis of pulpitis.

Objective To investigate the expression of nm23,integrin ? 5? 1 and VEGF in breast cancer(BC),and the effect of the expression on the development of BC. Methods The expression of nm23, integrin ? 5? 1 and VEGF in 51 patients with breast cancer were examined by immunohistochemistry,and their correlatios were ananlyzed. Results The low expression of nm23 in BC tissue was associated with lymph nodes metastasis. The low expression of integrin ? 5? 1 and the high expression of VEGF in BC tissues were associated with the lymph nodes metastasis and low differentiation of the tumor.The expression of nm23 and integrin ? 5? 1 in breast cancer tissues had positive correlation(P

During ischemia, the intracellular acidosis stimulates the Na +/H + exchanger. The increase in intracellular sodium secondary to increased Na +/H + exchange is reversed through exchange for calcium, resulting in calcium overload of the cells and producing cell injury and necrosis. NHE1 inhibitors exert a protective effect on myocardium subject to ischemia and reperfusion via reduction of Na +/H + exchange, attenuation of intracellular calcium accumulation and other mechanisms.

The paper described such functional modules as user login,prescription management,prescription review management,statistics of prescription review results,log management,user management and background data maintenance,as required in the circular Management Regulations on Hospital Prescription Review(Trial).Discussions in the paper focused on the structuring methods and design highlights of the prescription review system,which aim at improving the efficiency and accuracy of prescription review,and its networking with HIs and information sharing.

Up to present,the research of the early-warning system for the risk of the medical quality has been carried out both at home and abroad.The majority of these researches aimed at providing theoretical guidance for the scientific decision-making of local health departments.In order to construct quality of medical care evaluation indicators system.On the bases of intelligence research,drawing on a series of internationally recognized quality of medical care evaluation system.The majority of these researches aimed at providing theoretical guidance for the scientific decision-making of local health departments.However,the hospital-related research is very limited.Therefore,hospitals were selected as objects in this study.33 experts from 6 three"A" hospitals were selected.And by using a series of internationally recognized quality health care system as a source of reference and focusing on process control and standardizing of management,the early-warning system for the risk of the medical quality has been constructed based on the combination of theory and practice.At the same time,Delphi method was used to verify the results repeatedly to form a set of early-warning system for the risk of the medical quality which is objective,scientific and approved by experts.

As an important inflammatory cytokine, tumor necrosis factor-α (TNT-α) plays an important role in the process of cerebral ischemia-reperfusion. The discussion of the dynamic changes, the mechanism of neurotoxicity and antagonizing the therapeutic effect of TNF-α in the process of cerebral ischemia-reperfusion injury will provide theoretical basis for the treatment of cerebrovascular diseases.