Objective To study the expression site and the role of low-affinity nerve growth factor receptor(P75) in hepatic stellate cells(HSCs) of fibrotic human and rat liver.Methods Left lobe of liver was removed from CCl-4-induced rats fibrosis model and human fibrotic tissue sample was taken from liver puncture.The tissue specimen was routinely paraffin embedded and expression of P75 was detected by histochemical staining using polyclonal antibody against P75.Additionally,HSCs was cultivated and P75 was immunochemically assayed for P75 binding activity. Results P75 was seen abundantly on cell membrane of the cultured HSCs.In the fibrotic tissue of rat liver as well as human fibrotic liver P75 was also positively expressed.Similarly,P75 was found mainly a hepatocye surface in histochemical staining.P75 was not seen by histochemical staining in the normal tissue of human and rat liver.Conclusion P75 used as a new target for the treatment of hepatic fibrosis.

Psychological disorder has a high morbidity rate among patients with cardiovascular disease. Patients with cardiovascular disease associated with depression, especially post myocardial infarction and acute coronary syndromes associated with psychological disorder, have not only a poor prognosis, but also a high mortality . Therefore, comprehensive and perfect intervention is extremely important for patients with cardiovascular disease associated with psychological disorder. The intervention for such kind patients needs an integrated disease management. It needs to consolidate the treatment of body's disease to strengthen second-level prevention of heart disease, and pay attention to the intervention for psychological disorder as well.This paper aims to provide reference for reducing the negative effects of psychological illness in patients with cardiovascular disease during clinical treatment process, and make prospects about the significance of double heart medical model, through summarizing the interaction effects of psychological factors on patients with coronary heart disease, the significance ofdouble heartnursing mode and the specific nursing measures.

This paper introduces a typical network framework based on VPN.With emphasis on network's security and stability,it describes how to construct a remote network based on Internet with VPN by focusing on such facts as security,service quality and expansion,etc.Finally,some natures about VPN both in technique and security are shown.

Objective:To explore the effect between Xuezhikang and rosuvastatin on carotid intima-media thickness (IMT) .Methods :A total of 90 patients with carotid atherosclerosis were randomly and equally divided into control group ,Xuezhikang group and rosuvastatin group according to number table .After six-month treatment ,changes of blood lipid levels and IMT were compared among three groups .Results:Compared with before treatment ,there were significant improvements in blood lipid levels (except high density lipoprotein cholesterol ) and IMT after six months in Xuezhikang group and rosuvastatin group , P<0.05~ <0.01 ;compared with control group after treat-ment ,there were significant reductions in levels of triglyceride [ (2.12 ± 0.97) mmol/L vs .(1.17 ± 0.47) mmol/L , (1.87 ± 0.97) mmol/L] ,total cholesterol [ (5.95 ± 0.65) mmol/L vs . (5.14 ± 0.71) mmol/L ,(4.75 ± 0.64) mmol/L] and low density lipoprotein cholesterol [LDL-C , (3.64 ± 0.45 ) mmol/L vs . (3.01 ± 0.51 ) mmol/L , (2.15 ± 0.55) mmol/L] ,IMT [ (1.15 ± 0.15) mm vs .(1.01 ± 0.08) mm , (0.98 ± 0.02) mm] and total plaque score [ (6.10 ± 2.12) scores vs .(4.61 ± 2.08) scores , (4.59 ± 2.34) scores] in Xuezhikang group and rosuvastatin group , P<0.05~ <0.01 .After treatment ,there were no significant difference in above indexes between Xuezhi-kang group and rosuvastatin group ( P> 0.05 all) except that LDL-C level of rosuvastatin group was significantly lower than that of Xuezhikang group ( P< 0.05 ) .Conclusion:Both Xuezhikang and rosuvastatin can significantly improve carotid atherosclerosis ,and their therapeutic effects are equal .

Objective To investigate effect of leptin on nerve function in patient with Parkinson disease .Methods 68 cases patients with PD were selected and randomly divided into two groups, according to random number table, from October 2013 to December 2014 in the hospital.34 cases in control group were treated with conventional therapy, and 34 cases in experimental group were treated with leptin, with a consecutive treatment of twelve weeks.The score of activity of daily living scale ( ADL ) and mini-mental state examination ( MMSE ) were analyzed pre and post-treatment. ResuIts After treatment, the ADL score of experimental group was significantly lower than that of control group ( P<0.05 ) , MMSE score of experimental group was significantly higher than that of control group (P<0.05).The adverse reactions in both groups were minor.ConcIusion Leptin has good curative effect in treatment of Parkinson disease, and could significantly improve the neurological function of patients, with mild adverse reactions.

A method was developed for the determination of 39 polybrominated diphenyl ethers(PBDEs) from mono- to hepta-brominated in coastal sediment by gas chromatography/mass spectrometry. The sample was extracted with a solution of dichloromethane: n-hexane( 1:1,V/V) ,followed by ultrasonic extraction for 60 min at 25℃. The clean-up step was optimized using silica gel and alumina. The detection limit of the method was in the range of 0.003 - 0.10μg/kg. The results indicate that the average recovery of the internal standard was from 66.2% to 118. 6%. The relative standard deviation RSD( % ) was from 0. 8 % to 18. 2%. The method for the detection of 39 PBDE congeners in coastal sediment was sensitive,high reproducible with satisfactory recoveries.

Objective To study the diagnosis and treatment of iatrogenic bile duct injury(IBDI).Methods The clinical data of 92 patients with IBDI who had been admitted to our hospital from January 1994 to December 2007 were retrospectively analyzed.Results The diagnosis of IBDI was based on the clinical findings,diagnostic abdominocentesis and imaging examination.Sixteen patients gave up treatment because of economic reasons,and 2 patients who were complicated with sepsis and multiple organ dysfunction syndrome died shortly after being transferred to our hospital.Forty-eight patients were treated by Roux-en-Y choledochojejunostomy,14 by endoscopic treatment.2 by bile duet repair+T tube drainage,3 by percutaneous catheter drainage,1 by choledochoduodenostomy,4 by percutaneous transhepatic cholangiodrainage,and 2 by ultrasound guided percutaneous puncture drainage.Sixty-two patients were followed up for 4 month to 10 years(mean,3.6 years),and satisfactory results were obtained.Conclusions Detection of IBDI during operation and prompt repair yield favorable outcome.Roux-en-Y choledochojejunostomy is the best choice for bile duct injury complicated with biliary stricture.Endoscopic and interventional treatments are important auxiliary measures in the treatment of bile duct injury.

Objective To study the influence of serum containing Ziqi Ruangan Pill on inhibitory effect of alcohol LO2 and to investigate the regulation on apoptosis related genes and the protective mechanism for LO2. Methods Rabbit sera saline containing Ziqi Ruangan Pill was prepared. The apoptotic LO2 model was established by being cultured with alcohol. The effect of serum on the model was detected by MTT method. Identification of apoptosis was carried out using flow cytometry and Hoechst3325 fluorescent staining. The expression of apoptotic inducing gene,casepase3 and NF-?B was detected by Real-time RT PCR and Western-Blot. Results The serum containing Ziqi Ruangan Pill could regulate the expression of the casepase3,NF-?B and reduce the apoptosis. Conclusion Ziqi Ruangan Pill can protect the LO2 by regulating the expression of apoptosis stimulative gene casepase3 and NF-?B.

Objective:To make a synthetic comment on treatment of children cerebral palsy with acupuncture in recent 10 years in China.Methods:Related papers were gotten from the journals between 1999-2008 in Chinese journal full-test database and VIP information-Chinese technology journal database by computer retrieval.The collected 143 papers were analyzed from the terms of clinical acupoints selection,treatment methods,time,and mechanism,and then summarized the clinical effects of acupuncture in treating children cerebral palsy.Results:The acupoints selection was focus on scalp acupuncture functional area and body points(including scalp points).Beside acupuncture treatment,the combination of acupoint embedding therapy,acupoint injection,and massage were also used.The therapy time was different from 20min to 4h,and the treatment course was usually be counted by month.The effects of acupuncture treatment were considered to be significant through improving brain circulation and recovering brain cells' function. Conclusion:Acupuncture treatment had advantages in improving the child'limbs function,nevertheless further studies on the research methods,study design and mechanism were all needed.

Objective To study the effect of protein kinase C-nuclear factor-kappa B (PKC-NF-?B) signal transduction pathway on the umbilical sera-induced human umbilical artery smooth muscle cell (HUASMC) proliferation and the expression of precollagen Ⅰ, and Ⅲ mRNA in preeclamptic women. Methods HUASMC from normal pregnancy were cultured and generated. After 30 minutes of incubation with or without Polymyxin B (PMB), the umbilical serum from the preeclamptic women was added. Two hours later, the expression of Ⅰ-?B and NF-?B were detected by Western blot. After 48 h, the activity of HUASMC was evaluated by MTT, the cell cycle by flow cytomerty and the expression of precollagenⅠ,ⅢⅢ and bcl-3 mRNA by reverse transcription-polymerase chain reaction(RT-PCR). Results Ⅰ-?B level (0.3765?0.0321) and the percentage of G_0+G_1 phase cells (62.53?2.85)% in preeclamptic group were significantly lower than those in control group [(0.7857? 0.0296),(78.66?3.25)%, P