Objective: To observe the stability of tinidaz ole mixed with cefradin in glucose injection under room temperature or 37 ℃ for 8 hours.　Methods:Concentrations of tinidazole and cefrad in were determined by ultraviolet spectrophotometry and pH by a pH meter.　Results: The concentrations of tinidazole and cefradin and the pH of the solution did not change significantly under room temperature and 37 ℃ for 8 hours.　Conclusions: Tinidazole can be mixed with c efradin in glucose injection for clinical use under room temperature or 37 ℃.

Japan’s long-term care insurance takes grassroots governments as its insurer and the elderly and part of the non-elderly as its insured;The costs is shared by government、society和individual, and is, payed through isti-tutions, communities and hothes. Since its establishment, its security level has been increasing, the role of preven-tion has been rising, the status of community-based and home-based has been highlighting, and the cooperation a-mong relevant industry has been strengthening. But at the same time, its costs was soaring during the past decade, and the continued development of the system is facing transformation pressure now. Take this as a warning, the devel-opment of long-term care in China should learn its strong points, but also try to avoid its shortcomings.

Objective To evaluate the diagnostic role of the color Doppler flow image(CDFI) in malfunction of lower extremity perforator vein. Methods Using color Doppler flow image method,60 lower extremities were ex-amined,and compared with the results of the lower extremity vein angiography,then assessed the accuracy of CDFI method. Results 212 lower extremity perforator veins of malfunction were found,and compared with results of the lower extremity vein angiography, the sensitivity of CDFI method is 91.1%, the specificity of CDFI method is 85.8 % ,the accuracy of CDFI method is 91.9 %. Conclusion CDFI is useful for the diagnosis of malfunction of lower extremity perforator vein and can offer reliable bases to clinical operation.

Objective To explore the influence of psychosocial factors and depression-anxiety on the intellectual impairment in early period of cerebral infarct.Method We compared the correlation of the occurence of intellectual impairment and depression-anxiety,negative life events among 98 cerebral infarct dementia index cases and 80 cerebral infarct without dementia index cases and 87 control subjects.Result Anxiety states have 74.49％ cerebrol infarct dementia index cases,60％ cerebrol infarct without dementia index cases,and 14.94％ control subjects,Depression states have 74.49％ cerebrol infarct dementia index cases,51.25％ cerebrol infarct without dementia index cases,and 20.69％ control subjects,Negative life events have 78.57％ cerebrol infarct dementia index cases,63.75％ cerebrol infarct witout dementia index cases and 13.79％ contrbl subjects.Conclusion The mood and sensibilities problems of depression anxity(especially of anxity) have a neagative status on the intellegence impirment of cerebral infarct.The correlates of post stroke depression-anxity states analysis were paralysis and position of focal lesion.The negative life events influenced the occuring of intelligence imparement post-cerebral infarct without anxity-depression mood and sensibilities problemes.

An analytical method of reversed phase high performance liquid chromatography (RP-HPLC) was developed for simultaneous determination of 5-hydroxymethylfurfural and nine phenolic compounds (including (+)-catechin, (-)-epicatechin, chlorogenic acid, rutin, caffeic acid, protocatechuic acid, syringic acid, ferulic acid and p-coumaric acid) in 20 min.Ten components were detected and separated successfully by Diamonsil C_(18) column (150 mm x4.6 mm, 5 μm) at wavelength of 280 nm and column temperature of 42 ℃, with acetonitrile and 3% acetic acid solution as the mobile phase in gradient elution.The resultant correlation coefficients of the ten compounds were between 0.9911 and 0.9995 with detection limit from 0.2 to 0.5 mg/L, the RSD less than 2.4% and the average recoveries from 89.4% to 98.3%.These experimental results demonstrate that 5-hydroxymethylfurfural and the nine phenolic compounds in different rice wine samples can be determined with the new method for practical uses.

Objective To study the relationship between operative procedures and complication of colorectal carcinoma with acute colonic obstruction in the elderly patients. Methods 65 elderly patients undergoing emergency operation for acute colonic obstruction were analyzed retrospectively. One stage tumor resection and anastomosis was performed in 25 cases with right-side colonic lesion and in 26 cases with left-side colonic lesion, Hartmann operation in 8 cases, Dixon operation in 4 cases and proximal colostomy in 2 cases. Results In 65 cases, 13 cases of postopera-tive complications,the rate was 20.0% (13/65) ,which infected with toxic shock two cases,four cases of pulmonary infection,anastomotic leakage two cases, wound infection, split 2 cases, death three cases, the mortality rate was 4.6%. The rates of postoperative complications in right-side colonic lesion, left-side colonic lesion, Hartmann opera-tion and Dixon operation were 69.2% ,0,30.7% and 0 respectively(P < 0.05 ～ 0.01). Conclusion Acute obstruc-tion of colon cancer,the right-side colonic lesion were safe,feasible and few complications in colorectal carcinoma with acute colonic obstruction.

Objective To study the correlation between blood lactic acid level and PCIS scroing system.To study the relationship between dynamic lactic acid monitoring indexes and prognosis of critical patients.Methods Differences of mortality,incidence of MODS and shock,PCIS score between two groups were compared.The patients were divided into 3 groups by PCIS score to compare pathogenetic conditions prognosis, then to study the were divided into death group and survival group by the hospital prognosis. Differences of their lactic indexes and other clinical indicators which reflected organ/system status were compared, prognostic-significant lactic indexes were selected by mu fti-variable logistic analysis. Results Patients with elevated lactic acid level had more incidence of shock and MODS, higher PCIS scores and higher mortality, the differences had statistical significance compared with the normal lactic acid group. There were significant linear correlation between ICU lactic acid level, peak lactic acid level and PCIS scores respectively. For the patients with elevated lactic acid level, peak lactic acid level, 12h lactic clearance had significant correlation with prognosis. Conclusion PCIS scoring system had a significant linear correlation with lactic acid level. For the patients with elevated lactic acid level,peak lactic acid level,12h lactic clearance were good indicators to evaluate patients' prognosis.

Objective To investigate apoptosis of placental syncytiotrophoblast in patients with early-onset and late-onset severe preeclampsia. Methods From November 2008 to May 2009, 15 cases with early-onset severe preeclampsia and 15 cases with late-onset severe preeclampsia matched with 10 women with normal pregnancies as control at Sixth Affiliated Hospital of Shanghai Jiaotong University were enrolled in this study. Enzyme-linked immunosorbent assay was used to measure syncytiotrophoblast microparticles (STBM) levels in peripheral venous plasma. Caspase-3 were measured by western blotting.Results (1)STBM: the level of STBM of (71 ± 21 )μg/L in early-onset group were significantly higher than (42 ±30)μg/L in late-onset group and (26 ± 11)μg/L in control group(P ＜0.05). The level of STBM in late-onset group and control group did not reach statistical difference ( P ＞ 0. 05 ). ( 2 ) Caspase-3:the level of caspase-3 protein were 0. 85 ± 0. 61 in early-onset group and 0. 77 ± 0. 46 in late-onset group,which were all significantly higher than 0. 32 ± 0. 15 in control group( P ＜ 0. 05 ). However, the level of caspase-3 protein in early-onset group did not show remarkable difference compared with that in late-onset group (P＞0. 05). Conclusion STBM are shed into the maternal circulation in higher amounts in earlyonset preeclampsia, which indicated that early-onset and late-onset severe preeclampsia may have different etiology and pathogenesis.

Asymmetric dimethylarginine(ADMA) is an endogenous inhibitor of nitric oxide synthases(NOS),which is a risk marker of cardiovascular endothelial dysfunction.Recent studies found that ADMA was a predictor not only for acute cardiovascular disease,but also for critical illness and mortality.Studies have found that exogenous ADMA altered lung function and induced apoptosis in animal experiments and vitro experiments.The levels of plasma ADMA significantly increased in children with hypoxia,PPHN,brain damage,and preterm infants who required mechanical ventilation.ADMA could be a independent risk factor to predict neonatal complications and death.

Objective To compare two types of digestive reconstruction and postoperative complications after total gastrectomy in patients with gastric carcinoma. Methods Roux-en-Y esophagojejunostomy and P-type Roux-en-Y esophagojejunostomy were performed in 50 patients with gastric carcinoma after total gastrectomy. The operating time and postoperative complications were observed. Results The gastrointestinal symptoms for Roux-en-Y esophagojeju-nostomy and P-type Roux-en-Y esophagojejunostomy serve as : diarrhea ( 11.8% and 12.1% ), dumping syndrome (3.9% and 3.3% ) and reflux esophagitis(5.9% and 6.0% ) ,the incidence of the difference was not significant ( than,P>0.05 ). The postoperative complications in P-type Roux-en-Y esophagojejunostomy( 13.1% ) slightly higher that Roux-en-Y esophagojejunostomy( 11.1% ) ,but the difference was not significant( P>0.05 ). Roux-en-Y esoph-agojejunostomy average operative time(3.1±0.3)h was significantly reduced than P-type Roux-en-Y esophagojeju-nostomy(3.7±0.2)h(t=3.35,P<0.05). Conclusion Roux-en-Y esephagojejunostomy and P-type Roux-en-Y esophagojejunostomy is a suitable type after total gastrectomy for patients with gastric carcinoma, but the former opera-tive time was shorter.