Objective To investigate the clinical value of surem eystatin C and urinary transforming growth factorβ1(TGF-β1)in patients with severe eclampsism.Methods Serum cystatin C and urinary transforming growth factorβ1 were examined in 35 severe eclampsism women(experiment group,n=35)and normal late pregnant women(control group,n=35).Results The levels of serum cystatin C and urinary transforming growth factorβ1 were both significantly increased in experiment group than those in control group(both P<0.01).The serum level of cystatin C was positively correlative with urinary TGF-β1 level in experiment group(r=0.581,P<0.05).Conclusion The detection of serum cystatin C and urinary transforming growth factorβ1 is useful to assess renal function lesion in patients with severe eclampsism,which contributes to the diagnosis and treatment of severe eclampsism.

This article reviewed the theories and current researches of Ryodoraku Diagnostic System and pointed out its value in clinical studies, as well as the feasibility and significance of applying this system on the studies of electricity of meridians and collaterals.

Objective To study the effects of γ-aminobutyric acid ( GABA) and sertraline on the CABAA(α) receptor and GABAB receptor of hippocampus neuron in rats of depression induced by acute stress. Methods The cognitive function of male SD (Sprague-Dawley) rats was screened through Y-maze. Then rats were randomly divided into five groups. Except control group,rats were injected intraperitoneally with double-distilled, GABA,sertraline or GABA + sertraline respectively. They were exposed to the forced-swimming stress test,which was to make acute stress model of depression. The space memory function in the maze was measured. Then the number of GABA-like immunoreactive neurons in hippocampus CA1 ,CA3 ,DG areas of rats were investigated with immunohistochemist. Results Compared with the model group,the immobility time in the forced-swimming test and the latency in the maze could be reduced significantly after pretreatment with GABA and sertraline (P < 0.01). The positive cell populations of CA1 ,CA3 region and DG gate region in the model group were significantly reduced compared with the control group (P<0.01). GABA could only improve the positive cell populations of GABAB receptor in CA3 region (P<0.01). In the sertraline group,the positive cell populations in hippocampus were increased obviously more than it in model group(P<0.01). The positive cell populations of hippocampus in the GABA + sertraline group in CA1 ( (82.83±8.72),(78.08±5.67)),in CA3((92.83±9.35),(76.00±3.97)),in the gate of DG( (35.00 ±1.41) ,(33.33±4.36)) increased significantly to the other groups (P< 0.05 or 0.01). Conclusion Ectogenic GABA could improve the sertraline' s effects of antagonizing acute depression effectively and the space memory function,and its mechanism may be involved in strengthening the expression of GABAA(α) receptor and GABAB receptor in hippocampus.

ObjectiveTo study the effect of prevention and treatment on osteoporosis in climacteric women by sport therapy. Methods 120 climacteric women were separated into rehabilitation group(n = 60)and control group(n = 60). Rehabilitation group accepted the oxygensport therapy, calcium, adjusted food. Control group was given calcium and adjusted food.The changs in densify of lumbar vertebradensify ofwere observed two yeats after treatment. ResultsDensify of lumbar vertebra in the rehabititation groupincreased( but there was no sta-tistical significance) and decreased remarkably in control group( P ＜ 0. 05) . Densify of lumbar vertebra lumbar in rehabititation group in-creased than that of the control group( P ＜ 0. 05) . Conclusion Exercise therapy was effective in preventing and treating osteoporosis in cli-macteric worrn.

ObjectiveTo determine the nature of the food poisoning event that has not gathered the reason. Methods The descriptive epidemiology and case of illness are used to conduct a contrastive study. The bacterium study, the serology examination and the HACCP hygiene investigation method will be adopted as well for investigation and analysis. ResultThe attack rate of poisoning is 6.67%, incubation period 1.5~6.5h, center position incubation period 4.5h and the dangerous ratio of the food roasted chicken legs (OR) is 28.33, which is connected with the poison. The examination result is that the food is infected by the Vibrio parahaemolyticas to mix the Aeromonas sobria. The HACCP investigation determines that the food is poisoned by the overlapping contamination between the utensil and the food, or the dissemination of the disease carried by the staff. ConclusionThis the food poisoning event is caused by the Vibrio parahaemolyticas to mix the Aeromonas sobria.

Idiopathic intracranial hypertension,which often occurs among adult overweight women,is a kind of chronic intracranial hypertension without any certain causes.The patient usually present with headache and papilloedema,and normal of brain structure and the cerebrospinal fluid composition.As visual loss is the only serious outcome,both dietary management and medical treatment are helpful in the early time,but surgery should be required as soon as visual loss occurs.The recent clinical study on idiopathic intracranial hypertension was reviewed.

Objective: To study the relationship between endoplasmic reticulum stress (ERS) and adiponectin;to explore the role of ERS for increasing myocardial ischemic vulnerability in type 2 diabetes mellitus (DM) mice.
Methods: Type 2 DM model was established by high fat diet with streptozotocin (STZ) injection. A total of 35 C57BL/6J male type 2 DM mice were divided into 4 groups: ①Control group, n=5. ②Tauroursodeoxycholic acid (TUDCA) group,③Thapsigargin (TG) group and ④Normal saline group. The mice in Groups ②, ③, ④were fed by high fat and high glucose diet by injecting streptozotocin (STZ), in the last 3 weeks and respectively received intraperitoneal injections of TUDCA (250 mg/kg), thapsigargin (TG) (300μg/kg) and normal saline twice a day, n=10 in each group. Then myocardial infarction (MI) model was established in 5 mice from each group. 72 hours later, the MI ranges were measured, serum levels of adiponectin were detected, mRNA expressions of adiponectin and CHOP in myocardial tissue were examined.
Results: The MI range in TUDCA group (21.47 ± 2.85)%and in Normal saline group (39.92 ± 4.28)%were both lower than TG group (66.56 ± 8.15)%, both P<0.01. Before MI occurrence, serum levels of adiponectin in TUDCA group (79.25 ± 6.40) pg/ml and in Normal saline group (70.23 ± 4.15) pg/ml were both higher than TG group (62.64 ± 5.70) pg/ml, both P<0.01;serum levels of adiponectin in each group were higher than they were 72 h after MI. In each group, the mRNA expression and protein content of adiponectin in myocardial tissue were constant to serum adiponectin;while the mRNA expression and protein content of CHOP was opposite to serum adiponectin.
Conclusion: ESR could increase myocardial vulnerability in type 2 DM mice which might be related to down-regulating adiponectin expression.

Objective To establish a HPLC method to detect the propofol concentration of plasma in children undergoing cardiopulmonary bypass. Methods RP-HPLC with fluorescence detector was used in the study. Plasma samples were precipitated by the thymol-methanol solution, and centrifugated. 20?l of supernatant fluid was injected for analysis and separated by Dikma Diamonsil C18 column. The mobile phase was composed of acetonitril:water =56:44(V/V), flo rate was 1.0ml/min. Fluorescence detective wave lengths were ?ex= 276nm, ?em=312nm. HPLC machine was Agilent1100 series. Main column was Diamonsil C18(150?4.6nm,5um),guard column was Phenomenex Security(4mm?3mm). Results There was a good linear relationship within the range of 0.02～8.0mg/L(r=0.9993, n =9),the limit of detection was 5?g/L,average recovery was 96.99%,intra RSD(relative standard deviation )was between0.810% and 5.73%,inter RSD was between 0.932% and 7.337%. Conclusions The method is sensitive, simple, rapid, good linear relationship and precise. The experiment can be used for determination of propofol concentration in child undergoing cardiopulmonary bypass.

Objective To evaluate the effect of the assessment of intervention of rational use and special initiative of antineoplastic Chinese patent medicine in tumor ward of Nantong First People’s Hospital (hereinafter referred to as “the hospital”); To facilitate the rational use of medicine in clinical setting. Methods Medical records of discharged patients in April and October 2015 were collected, and various types of irrational use of Chinese patent medicine was analyzed and put into statistical analysis. Results The proportion of medical records of irrational use of Chinese patent medicine declined from 39.45% to 18.49%; the percent of irrational prescription order sheet declined from 11.49%to 3.45%;the differences of variors irrational medicine use before and after the intervention were statistically significant. Conclusion The status of the rational use of antineoplastic Chinese patent medicine in tumor ward is improved after half-year special intervention and correction initiative.

[Objective]To discuss the clinical experiences and unique views of professor CHEN Hongyu in treating chronic kidney disease at the fifth stage (non-dialysis).[Method]To analyze the professor CHEN Hongyu's understanding about the pathogenesis of chronic kidney disease and her academic point of view that treating from spleen and kidney. To sum up the clinical experiences in treating CKD5 (non-dialysis) according to invigorating the spleen and kidney, and analyze typical clinical case. [Result] Professor CHEN Hongyu considers that the root cause of CKD is the spleen and kidney deficiency and often accompanied by pathogenic dampness or blood stasis or turbidity toxin. Also rheumatic is an important risk factor for prompting disease activity and protracted course, which is easy to cause kidney deficiency, renal bi and is usually poor for the prognosis. Professor CHEN Hongyu considers protecting spleen and stomach and invigorating the spleen and kidney as the fundamental law, making good use of application of modern technology, emphasizing the combination of macro and micro, treatment based on syndrome differentiation,which has obvious curative effect to remit or eliminate clinical symptoms in patients with chronic renal disease, beneficial to delay the progress of the renal function and improve life quality.[Conclusion] Professor CHEN Hongyu 's experience in treating chronic kidney disease fifth stage(non-dialysis) is worth learning and spreading.