Objective:Toclone the OPGgene ofhuman beingand construct its recombinant adenovirus and then observe its effects on the differentiation of bone marrow stem cells(BMSCs).Methods:The cDNAs of human OPG in hepatic tissue were obtained by using RT-PCR method.The cDNAs then were recombined with competent homologous cells to form the AdOPG and the shuttle vector was used during this stage.The rabbit BMSCs were isolated and cultured in vitro,and were infected with AdOPG.The OPG expression of BMSCs was identified by RT-PCR and Western blot.The expression of ALP was detected by chromatometry and ALP dyeing five days after the infection.Results:The OPG gene of human being was successfully cloned and its sequence consistent with the Gene bank.The titre of constructed AdOPG can reach 109 efu/ml.The ALP activity of BMSCs was(21 024?507)IU five days after the infection of AdOPG,whereas the value was(3 079?89)IU in the control group and(2 156?78)IU in the blank control group.Conclusion:The OPG can induce the osetogeinc differentiation of BMSCs.

Gene Expression Profiling ; Humans ; Laser Therapy ; Lung Neoplasms ; genetics ; Microdissection ; methods ; RNA, Neoplasm ; isolation & purification

Biorefinery that utilizes renewable biomass for production of fuels, chemicals and bio-materials has become more and more important in chemical industry. Recently, steam explosion technology, acid and alkali treatment are the main biorefinery treatment technologies. Meanwhile, low temperature plasma technology has attracted extensive attention in biomass refining process due to its unique chemical activity and high energy. We systemically summarize the research progress of low temperature plasma technology for pretreatment, sugar platflow, selective modification, liquefaction and gasification in biomass refinery. Moreover, the mechanism of low temperature plasma in biorefinery and its further development were also discussed.
Biomass ; Biotechnology ; methods ; Biotransformation ; Chemical Industry ; Cold Temperature ; Plasma Gases

Abstract Globally, non-pharmacological interventions, such as keeping social distance and wearing masks, are the primary prevention and control strategy in the early stage of the coronavirus disease 2019 (COVID-19) pandemic. The successful development and the urgency use of the COVID-19 vaccines in some countries brings a new stage of combining immunization with non-pharmacological interventions in the fight with COVID-19. For the shortage of vaccines, the immunization strategies vary in countries. To end the pandemic, 47%-85% of the population should be immunized with effective COVID-19 vaccines, thus we should boost the yield of vaccines, formulate scientific immunization strategies, promote the mass immunization, improve the willingness of vaccination, and increase the equity of vaccine allocation; meanwhile, we should strengthen the surveillance of virus variation, vaccine effectiveness and safety, and keep on the vaccine research to copy with the potential threat of the virus variation.

OBJECTIVE:To observe the clinical effect of tiotropium bromide combined with salmeterol xinafoate and fluticasone propionate in the treatment of medium and severe COPD. METHODS:119 patients with medium and severe COPD were divided into control group(54 cases)and observation group(65 cases)according to admission order. Control group was treated with Salmeterol xi-nafoate and fluticasone propionate powder for inhalation 50 μg,bid;observation group was additionally given Tiotropium bromide powder for inhalation 18μg,qn. Both groups received 1 month of treatment. Lung function indexes as FEV1,FVC and IC,blood gas indexes as SaO2,PaCO2 and PaO2 and inflammatory factors as IL-8 and TNF-α,COPD assessment test(CAT)score were observed in 2 groups,and the occurrence of ADR was also observed. RESULTS:Before treatment,there was no statistical significance in above indexes between 2 groups(P>0.05). After treatment,FEV1,EVC,IC of observation group were increased significantly,SaO2 and PaO2 of observation group were increased significantly,while PaCO2,IL-8 and TNF-αlevels was decreased significantly;those index-es of observation group were significantly better than those of control group,with statistical significance(P<0.05). CAT score of 2 groups were decreased significantly compared to before treatment,and the observation group was significantly lower than control group,with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Tiotropium bromide com-bined with salmeterol xinafoate and fluticasone propionate shows good clinical efficacy and safety in the treatment of medium and se-vere COPD.

Objective To explore an applicable method for isolation and purification of Cryptosporidium parvum oocysts with high purity, recovery and vigor from mouse feces. Methods Four techniques were used for isolating and purifying C.parvum oocysts from mouse feces: modified saturated saline flotation, percoll gradient centrifugation, CsCl gradient centrifugation and the classical discontinuous sucrose gradient centrifugation. Oocysts received from the methods were used respectively to infect in vitro bovine fallopian tube epithelial cells (BFTE) and the development of the oocysts was examined under microscope after 48 h and 72 h cultivation. Results The number of oocysts received by the classical discontinuous sucrose gradient centrifugation [(2.86?0.08)?107] was significantly higher than that of percoll gradient centrifugation [(1.52?0.08)?107] (P0.05). Oocysts received from CsCl gradient centrifugation showed higher purity than those by discontinuous sucrose gradient centrifugation. Conclusion In comparison to the classical discontinuous sucrose gradient centrifugation, operation of the modified saturated saline flotation is easier and faster, and the purity of oocysts isolated by CsCl gradient centrifugation is higher.

Objective To explore the perioperative nursing of elderly patients undergoing esophageal hiatal hernia repair with laparoscope. Method Twenty elderly patients undergoing esophageal hiatal hernia repair with laparoscope were managed with holistic nursing and the key nursing points were summarized. Results After operations, 3 of them contracted with dysphagia, 5 with weakened gastrointestinal motility, 6 with pulmonary infections, 3 with dyspnea and 1 with thrombus in the deep veins of lower extremities. All the 20 patients got recovered successfully after pertinent treatments. 3 months follow-ups showed the clinical symptoms disappeared and barium meal showed no relapse of hernias. Conclusion Strengthened perioperative nursing of operations on esophageal hiatal hernia is beneficial for postoperative recovery and reduction of postoperative complications.

Objective To explore the common complications and methods of treatment in the malignant obstruction of upper alimentary tract with stent insertion. Methods With interventional procedure under fluoroscopic guidance fourteen self expanding stents were implanted in twelve patients, including nine with strictures or obstructions of esophagus, three with obstructions of gastroduodenum. Of the fourteen, nine were coated stents and five were uncoated stents. Results All stents were implanted successfully, but complications after the procedure occurred sometimes. There complications included: 1. Food bolus obstructed in three patients. 2. Chest pain occurred in four patients. 3. Tumour overgrowth or hyperplasia of granulation tissue in three patients caused restenosis of gastrointestinal tract. 4. Stent replacement in three patients. 5. Hemorrhage occurred in two patients (over 300 ml) causing threat to life. Conclusions The implantation of self expanding stent is a simple and effective method offering good palliation for upper alimentary tract obstructions. The complications shoud be treated correctly.

Objective To observe and compare the effect of one -time root canal therapy and routine root canal therapy applied in acute pulpitis treatment.Methods 450 cases of patients with acute pulpitis treated by author were selected with 450 teeth involved,and were divided into one -time group and routine group by using the principle of random.Each of the groups included 225 cases of patients,with 225 teeth involved.One -time root canal therapy was given in one -time group,while routine root canal therapy given in routine group.Short -term effects were com-pared 1 week after therapy,while long -term effects were compared 1 year after therapy.Results 1 week after thera-py,213 cases in one -time group were excellent,excellent rate was 94.67%,while 139 cases in routine was excel-lent,excellent rate 61.78%.And there was statistical difference between the two groups (χ2 =21.970,P <0.05). There were 214 cases in one -time group which were effective,and the total effective rate was 99.56%,and 225 cases in routine group were effective,with total effective rate of 100.00%,and there was no statistical difference between the two groups (χ2 =0.018,P >0.05).One year after therapy,211 cases in one -time group were successful,success rate was 93.78%,and 223 cases were effective,total effective rate 99.11%.217 cases in routine group were success-ful,success rate was 96.44%,and 224 cases were effective,with total effective rate of 99.56%.There were no statis-tical differences of success and effective rate between the two groups (χ2 =0.085,0.006,both P >0.05 ). Conclusion It is effective to apply one -time root canal therapy to treat acute pulpitis under strict indications,pain relieved,referral time reduced,operation difficulty decreased,compliance increased.And the long -term effect of one-time root canal therapy is the same as routine root canal therapy,and the short -term effect is better.It is worthy of clinical use.

Objective To develop an in vitro culture system for Cryptosporidium parvum in Madin-Darby canine kidney(MDCK) cell and observe its life cycle(from desquamate to oocyst).Methods Oocysts of C.parvum were co-cultured with MDCK cells in vitro.Culture condition was optimized and the life cycle of C.parvum investigated.Results The optimal culture conditions for C.parvum in MDCK cells were 2.0?105 cells cultured for 12 h, and infected by 1.0?105 oocysts in the Dulbecco′s Modified Eagle Medium with 5% FBS.Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly.Between the 60th and 72th hour, many oocysts emerged.Inoculated by the C.parvum-infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.Conclusion The culture system provides a model for propagation of the parasites and demonstrates a complete in vitro life cycle of C.parvum.