Aims: The marine actinomycetes are a rich source of novel bioactive molecules. Especially the exotic tropical marine habitat of the Kerala coastal region favours the actinomycete diversity. The present study focuses on the isolation, purification and morphological characterization of marine actinomycetes for the discovery of new bioactive compounds. Methodology and results: A total of 280 morphologically distinct actinomycetes were isolated from marine soil and sediments of 10 different isolation sites located along the coastal region of Thiruvananthapuram district, Kerala, India using standard microbiological techniques. The physicochemical analysis of the soil samples collected from different stations was also done. Conclusion, significance and impact of study Even though the soil/sediment samples were collected from geographically nearby places, the physicochemical parameters showed a significant variation. This may be one of the factors which may trigger the actinomycete diversity in these regions. The diversity of actinomycetes prevalent in this region could serve as a potential source for the discovery of novel biomolecules.
Actinobacteria ; Soil ; Chemical Phenomena

Alkaloids ; Antineoplastic Agents, Phytogenic ; Chemical Phenomena ; Chemistry ; Harringtonines

Gelatin and hydroxyapatite were introduced to polyvinyl alcohol (PVA) hydrogel with an attempt to enhance the performances of PVA hydrogel. Through a reiterative freezing-thawing methods, three kinds of PVA composite hydrogels were prepared. The mechanical performances of these composite hydrogels with the same PVA and HA content but varying gelatin content, such as tensile strength, elasticity modulus, creep curve, relaxation curve and friction coefficient were evaluated by using a computer-controlled universal electronic mechanical testing machine and a UMT-II frictional testing machine. The additional effects of hydroxylapatite and varying gelatin on the performances of composite PVA hydro-gels were analyzed. It was found that the gelatin content directly influenced the physical performances of PVA composite hydrogels; but no linear relationship was recorded. PVA composite hydrogel containing 2wt-% gelatin gave optimal results, i.e. tensile strength of 5.5MPa, compressive elastical modulus of 1.48MPa, creeping rate of 31% in 45 minutes, stress relaxing rate of 40.3%, and the starting friction coefficient of 0.332.
Elastic Modulus ; Friction ; Hydrogels ; chemical synthesis ; chemistry ; Mechanical Phenomena ; Polyvinyl Alcohol ; chemical synthesis ; chemistry ; Tensile Strength

OBJECTIVETo optimize the preparation technology of effective fraction of Catharsius molossus, and investigate the feasibility of process control by the physical and chemical characterization of extracts.

METHODUsed single-factor test method, choosed the main effective components of peptides and amino acids as indexes, combined with theology, chemistry, electricity, and other characterization, the study researched the prepared technology of effective fraction of C. molossus including extraction, concentration, separation, purification, drying and so on.

RESULTThe optimal preparation technology of effective fraction of C. molossus was that soaked an amount of crude drugs with three times of 85% ethanol for 48 h, added 10 times of 85% ethanol, percolated in 4 mL x min(-1) x kg(-1), collected percolation liquid, concentrated to 1:1 at 50-55 degrees C, removed fat by frozen, adopted DA201-C macroporous resin, used 1 BV of water and 4 BV of 70% ethanol as eluting agent, collected eluant respectively. The water part was concentrated and dried, then washed twice with 85% ethanol, collected washing liquid and mixed with 70% ethanol eluant. The product was obtained by concentrating and dring. At the same time, the liquid-phase system of each link was characterized in preparation of effective fraction of C. molossus, which showed that the surface tension related to polypeptide was essentially unchanged, and the conductivity related to salt decreased by about 90% with ineffective substances closely related to salt The results showed that the preparation technology maximumly retained the effective information, and removed the invalid information.

CONCLUSIONThe preparation technology of effective fraction of C. molossus is stable and reliable, and the process control in physico-chemical characterization of extracts is feasible.

Amino Acids ; analysis ; Animals ; Chemical Fractionation ; Chemical Phenomena ; Chemistry, Pharmaceutical ; Coleoptera ; chemistry ; Peptides ; analysis

The perfluorocarbon (PFC) nanoparticle is a sort of new drug carrier in recent years, and it has lots of unique properties such as chemical stability, favourable biocompatibility, high aerobic capacity, targeting action to tissues or cells and so on. In this paper, we summarize the physico-chemical properties of the PFC nanoparticles. We also show the research progress of the PFC nanoparticles as a kind of drug carrier for targeted therapy of cardiovascular system, nervous system diseases and tumor. Further more, its characteristics of being selectively in target cells, tissues, and rapid release of drugs are expounded. PFC nanoparticles, as drug carriers for targeted diagnosis and treatment, have good prospect and clinical application value.
Animals ; Biocompatible Materials ; pharmacology ; Chemical Phenomena ; Drug Carriers ; pharmacology ; Drug Delivery Systems ; Fluorocarbons ; pharmacology ; Humans ; Nanoparticles

This study aimed to evaluate the rheological characteristic of W/O microemulsion. Using the Low Shear-30 Sinus Rheometer, we assessed the effects of water percentage in microemulsion on the apparent viscosity of microemulsion at different shear rates and temperature, and on the viscoelasticity of microemulsion. The results demonstrated that with the increase of water, the apparent viscosities at four different shear rates increased exponentially. The fitted curve between water percentage and the apparent viscosity (eta) at low shear rate was steeper than the others. We found that viscous component (eta') and elastic component (eta") also increased exponentially when water percentage was increasing. This communication addressed the relationship between water percentage and eta, and discussed the physical meaning of the elastic component. The results suggest that both the viscous component and the elastic component present qualitatively physical nature, but the viscous component is more accurate than the other.
Chemical Phenomena ; Chemistry, Physical ; Elasticity ; Emulsions ; Phosphatidylcholines ; chemistry ; Rheology ; Shear Strength ; Viscosity

Due to the limitation of science and technology in ancient times, traditional Chinese medicines (TCMs) could have prepared only in traditional dosage forms, such as pills, powders, ointments and pellets. Though studies on multi-component TCMs have become one of major development orientations of TCM, the druggability of their preparations has always been neglected. On the basis of two key difficulties--the integration of studies on multi-component TCMs and TCM theory as well as the evaluation on their druggability, the essay proposes methods and technologies that can be adopted in studies on multi-component TCM preparations, including the characteristic physicochemical property of multi-component TCMs and its correlation with forming process, the release-modified micro pill preparation technology based on prescription-symptom-dosage, and the evaluation technology on release of release-modified micro pill components based on mathematical set model.
Chemical Phenomena ; Drug Compounding ; Drug Dosage Calculations ; Medicine, Chinese Traditional ; methods ; Models, Theoretical

The progress of research of the physical and chemical modification methods to improve the antithrombogenic property of biomedical polyurethane (PU) in the past five years is reviewed in this paper. The physical modification method includes physical blending, physical vapor deposition (PVD) and replication molding technique. Meanwhile, chemical modification method is focused on the covalent bonding to immobilized special molecular. Moreover, the covalent bonding method covered functionalizing the PU surface with tailor-made groups in the bulk and the activation of the surface to form unstable active sites for further reactions.
Animals ; Biocompatible Materials ; chemistry ; pharmacology ; Chemical Phenomena ; Fibrinolytic Agents ; chemistry ; pharmacology ; Humans ; Polyurethanes ; chemistry ; pharmacology

Purified hemoglobin was modified with pyridoxal 5-phosphate(PLP) and polymerized with glutaric dialdehyde(GDA) to get the products. By comparison of the physical, chemical and biological properties of different procedures for modification before and after polymerization, there is no significant difference in molecular distribution, methemoglobin(MetHb) concentration, oxygen carrier capacity, P50 and spectra. Furthermore, the procedure of modification after polymerization can save PLP greatly and decrease cost greatly. So the procedure of modification after polymerization is a better way in research and production. The addition of GDA could control the increasing of MetHb. By comparison on the physical, chemical and biological properties of different procedures, there is no significant difference in molecular distribution, MetHb concentration, oxygen carrier capacity and spectra between the procedure of adding GDA before PLP and that after PLP. But the P50 of adding GDA before PLP is much lower than that after PLP. So the procedure of adding GDA after PLP is a better way.
Blood Substitutes ; Chemical Phenomena ; Glutaral ; chemistry ; Hemoglobins ; chemistry ; Humans ; Methemoglobin ; chemistry ; Pyridoxal Phosphate ; analogs & derivatives ; chemistry

Several complexes with different mass ratios of hyaluronic acid to pectin were studied using AFM and IR at the room temperature kept by air conditioning. The results showed that hyaluronic acid and pectin were in the state of being complex and were consistent when the concentrations of hyaluronic acid and pectin were above 1 mg/ml and 5 mg/ml respectively, and the mass ratio was 1 : 5. The complex self-assembled to composite grain-shaped film. In comparison with simple hyaluronic acid, the viscosity of hylauronic acid and pectin complex was stronger, and water-solubility was lower. The complex has the bio-function of both hylauronic acid and pectin and has wide application potential in the field of biomedical engineering.
Chemical Phenomena ; Hyaluronic Acid ; chemistry ; Pectins ; chemistry ; Solubility ; Tissue Engineering ; Viscosity