Objective To investigate the effect of ultrashortwave diathermy on pulmonary hypertension in patients with chronic cor pulmonale(CCP), and its mechanism. Methods Eighty-seven cases of acute phase CCP were divided into 2 groups: an ultrashortwave treatment group, in which 45 patients were treated with both ultrashortwave diathermy and conventional treatment; and a control group, in which 42 patients received regular treatment. The plasma levels of VEGF, ET-1 and the PaO_2, mPAP and FEV1.0 in the two groups were measured before and after treatment. Results In contrast to the control group, the FEV1.0 and PaO_2 of the experimental group were remar-kably increased, while their VEGF, ET-1 and mPAP were significantly decreased after treatment. VEGF and ET-1 were negatively related to PaO_2, and positively related to mPAP. Conclusions Ultrashortwave therapy is effective in treating pulmonary hypertension in patients with CCP. The mechanism for this may involve the synthesis and release of VEGF and ET-1.

The recently successful cloning of extracellular calcium sensing receptor (CaR) has revealed its vital role in adjusting and maintaining the homeostasis calcium or even other mineral ion in the body. CaR belongs to the big family of G-protein coupled receptors, and has 1079 amino acids in its structure. CaR is broadly distributed in a variety of tissues, including parathyroid, thyroid C cells, kidney, intestine and bone. So far, three disorders of extracellular calcium homeostasis have been linked with abnormalities of CaR function. Agonists and antagonists of CaR may be proved useful in the treatment of the diseases due to calcium metabolic disorders.

Long non-coding RNAs are important regulators of gene expression.ANRIL which was coded on the Chr9p21.3 loci participates in the pathogenesis of tumor, coronary artery disease, type 2 diabetes mellitus and oth-er diseases.Multiple ANRIL isoforms are tissue-specific.ANRIL mainly functions through Polycomb proteins, while there are also other downstream targets.The mechanism of each isoform and the downstream pathways are hotspots incurrent researches.

Objective To develop a HPLC method for determining the content of riparsaponin in Homonoia riparia Lour.Methods The determination was performed on the HYPERSILBDS-C18(200 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-water(75∶25) at a flow rate of 1.0 ml/min.The detection wavelength was set at 203 nm,the column temperature was 30 ℃,and the column pressure was 10.0 MPa.Results There was a good linear relationship for riparsaponin over the ranges of 5 to 500 ?g/ml(r=0.999 8).The average recovery was 99.44%,and RSD was 0.52%(n=15).Conclusion The method has good reproducibility and high precision.It can be applied in quality control of Homonoia riparia Lour.

Summarizing the literature of syndrome differentiation and treatment of alcoholic liver disease (ALD) with Traditional Chinese Medicine (TCM) in recent five years,to investigate the realization and the laws of prescribing in this disease from the perspective of selecting drugs and forming formulas of TCM. This article summarized the characteristics of formula selection in treating ALD,and summed up the treatment principle of ALD and the realization of main syndrome by the principle of 'learning syndrome from formula','correspondence of syndrome with for mula'. The results showed that promoting blood circulation for removing blood stasis,tonifying consumption,promoting diuresis and percolating dampness were the main principle of prevention and treatment of ALD in recent years,and blood stasis,qi asthenia and phlegmatic hygrosis were the major pathogenesis of ALD. According to studying the literature of TCM,it was can be seen that the present treatment of ALD was still in the exploratory stage of clinical observation,it lacked multi-center,randomized and control prospective study of large sample,and judgment of curative effect were disunity,and objective evaluation criteria was lacked. These questions were worth further exploration.

Objective:To study the relative risk factor of bleeding again of patients with obscure gastrointestinal bleeding (OGIB) of negative result in capsule endoscopy(CE). Methods: 92 patients with OGIB whose extermination results of CE were negative were enrolled in the retrospective analysis, and they were divided into observation group(46 cases, with bleeding again) and control group(46 cases, without bleeding again). The data of casehistory of these patients were compared, respectively, and then these data were analyzed by using Logistic regression analysis.Results: The ratio of 50 years old or older than 50 years old and the number of abnormal blood coagulation of observation group were significantly higher than that of control group(x2=5.386,x2=14.331,P<0.05), respectively. And the number of taking Aspirin of observation group was significantly higher than that of control group, while the number of received special treatment of observation group was lower than that of control group(x2=7.180, x2=23.545,P<0.01). As the results of Logistic regression analysis, 50 years old or older than 50 years old, abnormal blood coagulation, taking Aspirin and non-receiving special treatment were the independent risk factors, respectively, for patients with negative results of CE.Conclusion: In the examination of CE, although the results of patients with OGIB were negative, they may blood again, and all of these factors including 50 years old or older than 50 years old, abnormal blood coagulation, taking Aspirin and non-receiving special treatment are the risk factors which can affect blooding again. These factors should be paid more attention.

Objective To investigate the expression and clinical significance of multidrug resistance gene(MDR1),multidrug-resistance-associated protein (MRP),lung resistance protein (LRP),glutathione S-transferase-?(GST-?)mRNA in non-small cell lung cancer. Methods From Oct.1996 to Oct.2001,RT-PCR was used to investigate the mRNA expression of the above four genes.We followed up the survival time one by one and calculated the probability of survival. Results The total positive rate of the four multidrug resistance gene was 53.1%,65.3%,67.3%,83.3%.There was a significant difference between the coexpression and the single positive rate( P 0.05).With the increasing expression of the MDR the curves moved to left,the survival time and probability reduced. Conclusion The coexpression of the MRG results in the MDR and affects the prognosis directly.It can be used as a criterion to evaluate the prognosis.

Phosphatidylserine (PS) is a phospholipid that is abundant in eukaryotic plasma membranes,has crucial biological functions.Under cell apoptosis, cells can not generate enough ATP for energy and the concentration of cytoplasmic Ca 2 +increases, resulting in PS eversion.Apoptosis and the clearance of apoptotic cells are essential processes in animal development and homeostasis.For apoptotic cells to be cleared, they must display aneat me signal, most likely PS exposure, which prompts phagocytes to engulf the cells.PS is exposed by the action of scramblase on the cell's surface in biological processes such as apoptosis and platelet activation.Once exposed to the cell surface, PS acts as an eat me signal on dead cells, and creates a scaffold for blood-clotting factors on activated platelets.The molecular identities of the flippase and scramblase that work at plasma membranes have long eluded researchers.Indeed, their identity as well as the mechanism of the PS exposure to the cell surface has only recently been revealed.We describe how PS is exposed in activated platelets and in apoptotic cells, and discuss the clearance of apoptotic cells.

6 score group using Wagner's QRS scoring system. CONCLUSIONS: Serum levels of certain inflammatory markers have some diagnostic value for ACS. The high levels of serum SAA, IL-6 and sCRP are not mainly caused by myocardial death, but by the inflammation in the multifocal unstable plague. [