The preliminary pharmacodynamic studies of the prepar ation of artificial Fel Serpentis and Bulbus Fritiliariae Cirrhosae and Shedan Chuanbei San (composed of natural Fel Serpentis and Bulbus Fri tiliariae Cirrhosae) were reported.The results showed that both of the above pre parations could prolong the cough latency and decrease cough frequeucies induced by ammonia within 3 min,promote the excretion of respiratory tract,increase the excretion of phenol red and inhibit auricular edema induced by xylol in mice. M eanwhile, the above two preparations could also inhibit Ach-induced tracheospasm in vitro.Therefore,the replacement of natural Fel Serpentis with the artificial in the preparation of Shedan Chuanbei San is reasonable.

Objective:To determine taurocholic acid (TCA) and taurodeoxycholic acid (TDCA) in artificial snake gall. Methods: HPLC was used in the quantitative analysis with spherisorb C 18 column, methyl alcohol 0.02 mol/L phosphric buffer (60∶40) as a mobile phase and detection wavelength at 210nm. Results: The linear range of TCA was from 0.28672 mg/mL to 2.8672 mg/mL, and the linear range of TDCA was from 0.26842 mg/mL to 2.6842 mg/mL. The average recoveries were 97.65% for TCA and 95.81% for TDCA. RSD were 0.79% and 3.27%, respectively. Conclusion: This method is simple and accurate.

To study the pharmacokinetics of cantide, an antisense oligonucleotide, and its metabolites after iv gtt administration in rhesus monkeys, a dual solid phase extraction pretreatment method coupling with non-gel sieving capillary electrophoresis analysis method was used for determination of cantide and its metabolites in plasma and their pharmacokinetic parameters were calculated. The pharmacokinetic behavior of cantide and its metabolites (M1 and M2) after iv gtt administration (8, 16 and 24 mg kg(-1)) in rhesus monkeys were investigated. After iv gtt administration of cantide to rhesus monkeys, cantide in plasma was eliminated rapidly and the terminal elimination half-life (t1/2) was 57.91-77.97 min, the correlation coefficients (r) to the dose of Cmax AUC(o-inf) and AUC(0-t) of the prototype was 0.9918, 0.9568 and 0.9773, respectively. The metabolites of cantide reached the Cmax following cantide immediately and the Cmax of metabolites were lower than that of the prototype. The CL(S) of cantide and its metabolites (M1 and M2) were 1.60-2.19, 5.92-8.58 and 6.07-8.78 mL min(-1) kg(-1), respectively. So, it is concluded that the Cmax of cantide and its metabolites increased with the dose, which is the same as their AUC(0-inf) and AUC(0-t). The CL(S) of metabolites were higher than that of the prototype. The MRT and t1/2 of metabolites in the high dose group increased obviously.
Animals ; Area Under Curve ; Electrophoresis, Capillary ; methods ; Female ; Half-Life ; Infusions, Intravenous ; Macaca mulatta ; Male ; Oligonucleotides, Antisense ; blood ; metabolism ; pharmacokinetics ; Phosphorothioate Oligonucleotides ; blood ; metabolism ; pharmacokinetics ; Solid Phase Extraction