Aim To investigate the effect of Tetramethylpyrazine (TMP) on the proliferation of airway smooth muscle cells (ASMCs). Methods Primary ASMCs of rats were cultured. The absorbance (A490) value of ASMCs treatment with platelet-derived growth factor (PDGF) in the presence of TMP was detected by MTTto observe the anti-proliferation of TMP. The levels of ERK1/2 and p-ERK1/2 proteins were determined by Western blot.Results In presence of the TMP with different concentrations (12.5,25,50,100 and 200 ?mol?L-1) at 6,12,24,36 and 48 hours,compared with control groups,the average inhibitory rates of cell proliferation in all groups were increased significantly (P

Objective To compare the efficacy of percutaneous transhepatic cholangial drainage(PTCD)and endoscopic retrograde cholangiopancreatography(ERCP)combined with self-expanding metal stent(SEMS)in treatment of obstructive jaundice(OJ).Methods From January 2022 to July 2023,125 OJ patients were selected and grouped into two groups based on treatment plans.The reference group consisted of 62 cases who underwent PTCD combined with biliary SEMS implantation,while the test group consisted of 63 cases who underwent ERCP combined with biliary SEMS implantation.The remission rate of jaundice,liver function,cellular immune function,and complications were compared.Results There was no statistically significant difference in the relief rate of jaundice between the test group(90.48%,57/63)and the reference group(87.10%,54/62)(P＞0.05).After treatment,six measures of liver function in the test group were lower than those in the reference group,three indexes of cellular immune function in the test group were higher than those in the reference group,the differences were significant(P＜0.05).The total incidence of complications in the test group(9.52%,6/63)showed no statistically significant difference compared to the reference group(12.90%,8/62)(P＞0.05).Conclusion ERCP combined with biliary SEMS implantation is superior to PTCD combined with biliary SEMS implantation in improving liver function and enhancing cellular immune function,and there is no difference in jaundice relief rate and incidence of complications compared to PTCD combined with biliary SEMS implantation.

Immunology courses are offered in the majors of biology,medicine,animal husbandry and food in Chinese colleges and universities.Due to the characteristics of multi-dimensional composition of immunology knowledge,most students are not easy to understand the logical relationship between key points and knowledge points in learning,and will encounter problems such as unable to keep up with the teaching pace,lack of confidence in learning,feeling complex association between the knowledge points of chap-ters,and not fully understanding the immunology knowledge points.This paper summarizes the teaching methods of heuristic,main line and case in Immunology teaching,so as to solve the difficulties in learning,clarify learning ideas,improve learning confidence and learning effectiveness.

Objective:To investigate the effects of mindfulness therapy on blood glucose control and quality of life in older adult patients with diabetes mellitus and cognitive impairment.Methods:A total of 313 patients with type 2 diabetes mellitus and cognitive impairment aged over 75 years who received treatment in Xinxiang First People's Hospital from August 2018 to July 2020 were included in this study. They were randomly allocated to the intervention group ( n = 157) and control group ( n = 156). All patients underwent treatments for diabetes mellitus and underlying diseases. The intervention group was subject to 8 weeks of mindfulness therapy. The percentage of patients with qualified glycosylated hemoglobin in human whole blood and 36-Item Short Form Health Survey questionnaire score were compared between the two groups. The Pearson correlation was performed. Results:After 8 weeks of treatment, the percentage of patients with qualified glycosylated hemoglobin in human whole blood in the intervention group was 43.95%, which was significantly higher than the level (25.48%) at the recruitment and the level (30.77%) in the control group ( χ2 = 12.17, P = 0.002; χ2 = 5.80, P = 0.016). The quality of life score was significantly higher in the intervention group than in the control group [(72.16 ± 14.08) points vs. (64.37 ± 15.49) points, t = 4.65, P = 0.001]. Conclusion:Mindfulness therapy can regulate blood glucose level and improve quality of life in older adult patients with diabetes mellitus and cognitive impairment.

BACKGROUNDUp to now, locally advanced non-small cell lung cancer simutaneously involving carina, heart and great vessels is still regarded as contraindication for surgical treatment. However, the prognosis is very poor in these patients treated with chemotherapy and/or chemoradiotherapy. The aim of this study is to summarize the clinical experiences of carinoplasty combined with heart and great vessel plasty in the treatment of 84 patients with locally advanced non-small cell lung cancer involving carina, heart and great vessels or both in our hospital.

METHODSFrom March, 1988 to December, 2004, carinal resection and reconstruction combined with heart, great vessel plasty was performed in 84 patients with locally advanced non-small cell lung cancer involving carina, heart and great vessels simutaneously. The operative procedures in this series included as follows: (1) Right upper sleeve lobectomy combined with carinal resection and reconstruction, and right pulmonary artery sleeve angioplasty in 9 patients; (2) Right sleeve pneumonectomy combined with partial resection and reconstruction of left atrium, and superior vena cava resection and Gortex grafts in 3 cases; (3) Left upper sleeve lobectomy combined with carinoplasty, left pulmonary artery sleeve angioplasty and partial resection and reconstruction of left atrium in 3 cases; (4) Right upper sleeve lobectomy combined with carinoplasty, right pulmonary artery sleeve angioplasty and partial resection and reconstruction of left atrium in 10 cases; (5) Left upper sleeve lobectomy combined with carinoplasty and left pulmonary artery angioplasty in 9 cases; (6) Left upper sleeve lobectomy combined with carinoplasty, left pulmonary artery sleeve angioplasty and resection of the aorta arch sheath in 6 cases; (7) Right upper-middle sleeve lobectomy combined with carinoplasty and right pulmonary artery sleeve angioplasty in 3 cases; (8) Left upper sleeve lobectomy combined with carinoplasty, left pulmonary artery angioplasty, resection of the aorta arch sheath and partial resection and reconstruction of left artium in 8 cases; (9) Right upper sleeve lobectomy combined with carinoplasty, right pulmonary artery angioplasty and partial resection and reconstruction of left atrium in 4 cases; (10) Left sleeve pneumonectomy combined with partial resection and reconstruction of left atrium in 3 cases; (11) Right upper-middle sleeve lobectomy combined with carinoplasty, right pulmonary artery angioplasty and superior vena cava resection and reconstruction with Gortex grafts in 23 casese; (12) Right sleeve pneumonectomy combined with partial resection and reconstruction of left atrium in 1 case; (13) Right upper-middle sleeve lobectomy combined with carinoplasty, right pulmonary artery angioplasty and partial resection and reconstruction of left atrium in 1 case; (14) Right upper-middle sleeve lobectomy combined with carinoplasty, right pulmonary artery angioplasty and right inferior pulmonary vein sleeve resection and reconstruction in 1 case.

RESULTSThere were two operative death in this series. The operative mordality was 2.38%. A total of 32 patients had operative complications. The incidence of operative complications was 38.10%. The 1-, 3-, 5-and 10-year survival rate was 81.34%, 59.47%, 31.73% and 24.06% respectively.

CONCLUSIONS(1) It is feasible in technique that carinal resection and reconstruction combined with heart, great vessel plasty in the treatment of locally advanced non-small cell lung cancer involving carina, heart and great vessels simutaneously; (2) Multiple modality therapy based on carinal resection and reconstruction combined with heart and great vessel plasty can remarkably increase the survival rate, and improve the prognosis and quality of life in patients with locally advanced non-small cell lung cancer involving carina, heart and great vessels.

Genome-wide physical protein-protein interaction(PPI)mapping remains a major chal-lenge for current technologies.Here,we reported a high-efficiency BiFC-seq method,yeast-enhanced green fluorescent protein-based bimolecular fluorescence complementation(yEGFP-BiFC)coupled with next-generation DNA sequencing,for interactome mapping.We first applied yEGFP-BiFC method to systematically investigate an intraviral network of the Ebola virus.Two-thirds(9/14)of known interactions of EBOV were recaptured,and five novel interactions were discovered.Next,we used the BiFC-seq method to map the interactome of the tumor protein p53.We identified 97 interactors of p53,more than three-quarters of which were novel.Furthermore,in a more complex background,we screened potential interactors by pooling two BiFC libraries together and revealed a network of 229 interactions among 205 proteins.These results show that BiFC-seq is a highly sensitive,rapid,and economical method for genome-wide interactome map-ping.