Objective To study the differences of CXCR7 expression between in osteosarcoma tissues and normal peri-osteosarcoma tissues, and observe the effect on cell proliferation and inflammatory cytokine PGE2 secretion in MG63 cells when it had been transfected with pEGFP-Adv-CXCR7 vector. Methods CXCR7 expression in osteosarcoma tissues, cells and normal peri-osteosarcoma tissues detected by RT-qPCR. The CXCR7 mRNA expression, cell proliferation and PGE2 secretion of MG63 cells after transfected with pEGFP-Adv-CXCR7 expression vector were evaluated by RT-qPCR, MTT and ELISA, respectively. Results The expression rate and quality of CXCR7 mRNA in osteosarcoma tissue were 93.80% and (2. 221 ±0. 112), which significantly higher than those of normal peri-osteosarcoma tissues 45.71% and (0. 3448 ±0.098). Low expression of CXCR7 in MG63 cells was also observed. The proliferation and PGE2 secretion of MG63 cells were significantly increased at the time point of 48, 72 and 96h after pEGFPAdv-CXCR7 expression vector transfected into MG63 cells ( P < 0. 05). Conclusion The expression of CXCR7 mRNA in osteosarcoma tissues were significantly higher than normal peri-osteosarcoma tissues. CXCR7 high expression may have a negative effect on the progress of osteosarcoma.

ObjectiveTo investigate the early diagnostic value of serum level of myelin basic protein (MBP) on spinal cord injury (SCI).Methods54 rabbits were divided randomly into slight, moderate and severe SCI groups, and SCI degrees of each group were judged by cortical somatosensory evoked potential, histopathologic examination and Tarlov′s score. ELISA was used to determine the serum levels of MBP before and after SCI.ResultsThe serum levels of MBP of rabbits in three groups were significantly different at 24th, 48th and 72nd hour after injured (P<0.05), but there were not differences at 7th day (P>0.05).ConclusionThe serum level of MBP can be used as a early diagnostic marker for SCI.

BACKGROUND:It remains hard to judge characterization of lesion tissues using two-dimensional sectional image at present. Medical three-dimensional reconstruction technique could obviously improve work efficiency and accuracy of medical working staff on disease diagnosis.
OBJECTIVE:To develop a three-dimensional medical visualization system can rebuild three-dimensional model of corresponding parts by reading DICOM data, and observe the appearance of affected hip joint using reconstructed models.
METHODS:A three-dimensional (3D) medical visualization system was developed using the MFC in a PC with Windows XP operating system, development environment VC++6.0, VTK 5.6 instal ation, and necessary settings were made. Precise procedures are as fol ows: ① create a drawing object; ② create a drawing window, and draw objects was added in draw window; ③ read the CT image sequences, and set the path of the read image sequence; ④ isosurface (generate triangular facets) were extracted using MC algorithm, depending on the gray, skin and bones. Data of the input image sequence were set;gray value of tissue contour was set; ⑤the establishment of a V-belt objects and data mapping object; ⑥ graphics rendering, to receive the properties of the geometric data, and a different colors and transparency of bones and skin were set; ⑦ the viewing position was set to observe the position of the object and focus; ⑧ to create a human-computer interaction function.
RESULTS AND CONCLUSION:VC++6.0 and VTK could meet the needs of medical 3D visualization systems development. Developed 3D visualization system software could perform 3D reconstruction through reading CT images of DICOM format files. Thus, the relevant parts of the reconstructed 3D model could be observed by rotating, zooming, and panning so as to visual y observe the bone structure of hip joint, fractured appearance and type. It provided a reference for relevant therapy and operation.

BACKGROUND:It is difficult to adjust the anteversion angle of cementless hip joint. Some areas rely on the intraoperative three-dimensional navigation technique to ensure the accuracy of the anteversion angle, but its high cost limits the promotion prospects. OBJECTIVE:To design a kind of artificial femur head, which has special functions to freely adjust the anteversion angle of artificial femur head during operation and lock prosthesis handle to prevent femur prosthesis dislocation and looseness, and to intuitively display the special design concept of this kind of artificial femur head through the three-dimensional dynamic image aided by computer. METHODS:According to the design idea, AutoCAD software was used to get the design drawings for artificial femur head. The three-dimensional modeling was performed by 3DMax software in order to observe the form and degrees of verisimilitude of model. RESULTS AND CONCLUSION:The AutoCAD software was used to draw out the design drawings of artificial femur head according to the design requirements and design idea:the prosthesis handle had three lock hole channel and its basal part was for scale, and rotating the scale could change the anteversion angle of prosthesis neck;the shape of the prosthesis neck was dentate cylindrical. The 3DMax software was used to build the three-dimensional model of the artificial femur head, and the design, form and degrees of verisimilitude of the model comply with the design requirements. The whole structure of the three-dimensional model of artificial femur head is clear and the design is reasonable, which can provide a theoretical reference for further design of artificial femur head.

Objective To evaluate the feasibility of endoscopic transoral-transpharyngeal approach to the upper cervical. Methods Anatomic characteristics were observed and measured in the anterior column of 50 dry atlas and axis specimens. Conventional and endoscopic methods to decompress the spinal cord and excise the cartilage surface of the atlantoaxial joint by transoral-transpharyngeal approach were taken respectively in two groups of cadaveric heads and necks. All the cadaveric specimens were then open dissected to evaluate endoscopic operation methods, decompression size and the "safe zone". Results The anterior arch of atlas was of a length of (19.8?2.3) mm, the height of odontoid was (15.9?1.9) mm, the width (10.5?0.6) mm, and the thickness (11.5?1.9) mm; the maximal transverse diameter of superior facet of axis was (15.1?1.6) mm, and the anteroposterior one was (17.7?1.3) mm. The anterior tubercle of the atlas could be acted as landmark leading to the endoscopic atlantoaxis surgery. The arch could be drilled either from the tubercle to the lateral side or broken from the junction to the lateral mass. Endoscopic odontoid dissection should begin at the apex of the odontoid, and proceed inferiorly. It was necessary to move or slope the working tube to explore atlantoaxial lateral joint and dissect its cartilage, but the width and depth of cartilage dissection should be limited to 12 mm and 10 mm in order to avoid damage to vertebral artery and spinal cord. Measurements after postoperative open dissection showed that endoscopic decompression size were not significantly different from that of conventional method. There was a "safe zone" in the front of atlantoaxis of transoral-transpharyngeal approach, with (45.9?3.6) mm wide and (29.4?2.5) mm high. Conclusion Endoscopic transoral-transpharyngeal approach to the upper cervical is technically feasible, which had a good exploration and could get the same decompressing size with conventional transoral-transpharyngeal approach.

0 05 ) . But the results in the adolescent adult group undergone MED were better than the aged group (p0 05 ).Intravertebral complications had a significant effect on operative results (p

BACKGROUND: It was reported that the reason why the fracture healing become more and more difficult with the increasing age may be associated with the varied gene regulation of bone marrow mesenchymal stem cells differentiation into osteoblast and lipoblast. However, the exact mechanism under it remains poorly understood.OBJECTIVE: To explore the association of the age-related changes on expression of peroxisome proliferator-activated receptor γ (PPARγ) and core binding α1 (Cbfα1) with the age-relatad fracture healing impairment, we studied the expression changes of PPARγ and Cbfα1 during aging fracture healing.DESIGN, TIME AND SETTING: A randomized control animal trial was performed in the Third Xiangya Hospital of Central South University between October 2007 and Febuary 2008. MATERIALS: Six male SD rats of 3 months and the other six of 23 months, randomly-selected, were divided into two groups: the old experiment group (23 months) and the young control group (3 months). METHODS: Transverse osteotomies on the middle-upper parts of left tibiae were performed to the rats, with the self-made extemal mini-fixator implanted there simultaneously. Tractions were taken twice a day (0.2 mm/d) for 14 days from day 2 till day 15 post operation. On day 15 post operation, rats were sacrificed to harvest left tibea samples. MAIN OUTCOME MEASURES: Both imaging examination and histological observation were performed to the fractures of rats. The PPARγ and Cbfα1 expression were detected with reverse transcription-polymerase chain reaction (RT-PCR) technique. RESULTS: All samples were involved in the results analyses. The imaging examination showed that many osteotylus generated between the broken ends of fractures in the young control group; that the bone formation of the young control group was much better than that of the old experiment group. The histological observation showed that large amounts of osteotylus growth occurred to every animal in the young control group and their membrane bone formation was remarkable; comparatively, the osteotylus growth in the old group was weakened dramatically, and only fibrous joints were seen. The RT-PCR detection showed that differences of significance existed in both PPARγ and Cbfα1 mRNA expressions between the two groups, with the much higher Cbfol mRNA expression in the bone marrow of rats in the young group and the much higher PPARy mRNA expression in the bone marrow of rats in the old group.CONCLUSION: Rats of different ages show different fracture healing abilities, i.e. the higher ages, the lower fracture healing abilities. In addition, expressions of PPARγ and Cbfα1 in the bone marrow of rats alter with the increasing age, which indicates that there are certain associations between the changed expression level of the two factors and the fracture healing impartment.

Objective To establish an optimum DNA extraction method for Chinese traditional medical herbs in order to meet necessary for DNA barcoding research.Methods Four Chinese traditional herbs, Glycyrrhiza uralensis Fisch, Phellodendron Chinensis Cortex, Cistanche tubulosa Wight and Cistanche deserticola Ma were chosen as the experimental materials, the DNA was extracted by 6 different kinds of DNA extraction method, including the improved method of high-salt combined low-pH,the improved method of SDS,CTAB method,PVP method,PlantZol Kit and Ezup Kit, the quality of DNA was investigated by ultraviolet spectrophotometry,agarose gel electrophoresis and PCR amplification by using specific primers of ITS2 and psbA-trnH. ResuIts The quality of the DNA was better than other four kinds of methods by the improved method of high-salt combined low-pH and Ezup Kit, the value of OD260/OD280 was between 1.7 ~1.9,the yield of DNA was the highest by the PlantZol kit , followed by the improved method of high-salt combined low-pH( P <0.05 ) , but the purity of DNA was poor by the PlantZol kit.The DNA electrophoresis tests showed that the DNA integrity of Glycyrrhiza uralensis Fisch and Cistanche tubulosa Wight were better with the improved method of high-salt combined low-pH, the improved SDS method, the CTAB method and the PlantZol kit.The DNA of Phellodendron Chinensis Cortex and Cistanche deserticola Ma were extracted by the six methods appeared diffuse status in the lanes.But only the improved method of high-salt combined low-pH could make the PCR amplification of the success rate 100% by using specific primers of ITS2 and trnH-psbA.ConcIusion The DNA extraction method of high-salt combined low-pH can be used to establish the Chinese DNA barcoding which has the advantages of lower cost, simpler procedure and less time.

Objective To prepare poly (lactic-co-glycolic acid) (PLGA) microspheres that contain recombinant epidermal growth factor and then evaluate the effect of these sustained release in vitro.Methods Human serum albumin (HAS) microspheres were prepared with a double-emulsification solvent evaporation method.Experiment was designed to optimize the preparation condition of recombinant human epidermal growth factor (rhEGF)-loaded microspheres.Characters of optimal rhEGF-loaded microspheres were analyzed.In-vitro dissolution tests were performed on the microsperes.Results The speed of first emulsification,the concentration of PLGA,the concentration of polyvinyl alcohol (PVA),and the ratio of inner water to organic phase affected the particle size and the encapsulation ratio.The polymer's individual specificities especially density,molecular weight,polymerization,the diameter of microsphere,and the encapsulation efficiency were the crucial factors to influence the speed and time of drug release from microspheres in vitro.The optimal microspheres possessed a smooth and round appearance.It also showed good lente liberantes effect in vitro.Conclusions The optimal microspheres possessed a smooth and round stable appearance and showed good lente liberantes effect in vitro.This technique was simple and had a good reproducibility.

Objective To investigate the feasibility and effectiveness of fast track surgery (FTS) in neurosurgery. Methods One hundred fifteen patients who underwent neurosurgery surgery in Henan Province People's Hospital from June 2012 to March 2014 were enrolled in this study. All the patients were divided into FTS group (62 cases) and the tra?ditional operation group (53 cases). The clinical index, postoperative hospital stay and hospitalization cost were compared between the two groups. Results The clinical index were significantly lower in FTS group than in traditional operation group (P<0.05). Length of hospital stay (days) and hospitalization cost of FTS group were significantly shorter and lower in FTS group compared with traditonal operation group (8±1 vs. 11±2 days and RMB 4.58 ±0.75 vs. 5.78 ±0.64 ten thou?sand, respectively) (P<0.05). Conclusion FTS in neurosurgery operation is an all-new concept for surgery which can ef?fectively reduce postoperative complications, shorten length of hospital stay, decrease hospitalization cost and promote postoperative recovery.