1.Quantitative Analysis of Bcl-2,Bax, Bcl-xl Expression in Basal Cell Carcinoma with Immunohistochemistry
Liehua DENG ; Gang ZHAO ; Chaoyong XIA
Chinese Journal of Dermatology 1995;0(03):-
Objective To explore the roles of Bcl-2, Bax, Bcl-xl proteins in the carcinogenesis of basal cell carcinoma(BCC). Methods Forty-six BCC cases were divided into 2 groups (21 in non-aggressive BCC group and 26 in aggressive BCC group) according to their pathological features. Bcl-2, Bax and Bcl-xl were evaluated in formalin fixed, paraffin embedded specimens by immunohistochemical methods and analyzed by image-analysis methods to obtain average optical density (AOD) of each protein for each case. The AOD data of 3 proteins and Bcl-2/Bax ratios were then compared between the 2 BCC groups. Results The level of Bcl-2 expression and Bcl-2/Bax ratio of non-aggressive BCC group were higher than those of aggressive group,(P=0.005 and 0.044, respectively). For the expression level of Bcl-xl and Bax, there was no statistically difference between the two groups (P=0.097 and 0.979, respectively). Conclusion Bcl-2, Bax,Bcl-xl proteins might participate in the carcinogenesis and development of BCC.
2.Tubulin expression of basal cells in patients with Alzheimer's disease and vascular dementia
Jihua XIAO ; Chaoyong XIA ; Yanbin JIA ; Pei ZHENG
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To investigate quantitatively the tubulin expression of basal cells in patients with Alzheimer's disease(AD) and vascular dementia(VD) and evaluate its significance in diagnosing Alzheimer's disease. METHODS: ?-tubulin of basal cells was examined using LSAB immunohistochemical technique, and analyzed qualitatively and quantitatively by image analyzer. RESULTS: The integrated absorbance (I A ,261.43?25.21) and the average absorbance (A A ,1.89?0.14) in the AD group were significantly higher than those (I A ,120.55?19.71 and A A , 0.85?0.14, respectively)in the VD group ( P
3.EXPRESSION OF INTERRELATED POLYPEPTIDES AND THEIR REGULATORY FUNCTION IN MOUSE FETAL LUNGS
Xian LI ; Zhongxin HUANG ; Chaoyong XIA ; Li QIN ;
Acta Anatomica Sinica 1954;0(02):-
Objective To observe the distributions of epidermal growth factor receptor(EGFR),transforming growth factor ?(TGF ? 1,? 2,? 3)and thyroid transcription factor 1(TTF 1)in developing lungs,and to discuss their functions and relationships each other. Methods The expression of EGFR and TGF ? was examined in mouse fetal lungs by immunohistochemistry.TIGER image analysiser was used in TTF 1 quantitative study. Results 1.In the early developmental phase,EGFR localized in the epithelium of airway surface,TTF 1 was expressed predominantly in the nuclei of distal lung buds and TGF ? was expressed in the bronchioles with localization patterns.2.At the late developmental phase,the positive reactions of EGFR and TTF 1 were mainly detected in alveolar cells.TGF ? expressed in mesenchymal at high levels.3 The average optical density(A A )of TTF 1 in the distal epithelial cells increased gradually during the development,while their volume(VOL)and volume integrated optical density(VI A )all decreased.Conclusion\ The functions of EGFR,TGF ? and TTF 1 might be respectively different at different developmental stages.They not only stimulate the process of branching morphogenesis,but also modulate epithelial maturation and differentiation.\;[
4.Expression of Matrix Metalloproteinase-9 and Its Tissue Inhibitor in Cutaneous Squamous Cell Carcinoma
Jun FENG ; Liehua DENG ; Gang ZHAO ; Tao LU ; Guangyu JIANG ; Chaoyong XIA ; Jie LIU ; Ze LIN
Chinese Journal of Dermatology 2003;0(07):-
Objectives To investigate the expression of matrix metalloproteinase-9 (MMP-9) and its tissue inhibitor (TIMP-1) in cutaneous squamous cell carcinomas (SCC) and the relationship between the expression and invasive growth and metastasis of SCC. Methods Immunohistochemical method of streptavidin-peroxidase (SP) was used to detect the expression of MMP-9 and TIMP-1 proteins on the paraffin embedded sections of 65 patients with cutaneous SCC and 5 cases of normal epidermis. The immunohistochemical results were analyzed quantitatively using an image analysis system. Results MMP-9 and TIMP-1 proteins were diffusely expressed on the tumor nests and the mesenchymal cells around the nests, while in normal epidermis MMP-9 and TIMP-1 were weakly positive. The expression level of MMP-9 protein and the ratio of MMP-9/TIMP-1 were higher in aggressive SCC group than those SCC in situ group (t = 2.33 and 2.36, respectively, P
5.Disruption of circadian rhythms inhibits wound healing of corneal epithe-lium in mice
Peng LIU ; Jun LIU ; Yunxia XUE ; Fang SONG ; Hanqing WANG ; Ting FU ; Chaoyong XIA ; Zhijie LI
Chinese Journal of Pathophysiology 2015;(3):499-504
[ ABSTRACT] AIM: To observe the effect of circadian rhythms on wound healing of mouse corneal epithelium. METHODS:The C57BL/6 male mice were used in the study.A part of corneal epithelium (2 mm in diameter) was struck off by a golf-like knife to form a round wound area.The dynamics of epithelial healing in the wound area were ob-served under microscope with fluorescein staining.In addition, with related antibodies and DAPI, the dynamic changes of the neutrophils, platelets and dividing cells were also investigated.RESULTS:The healing rates in LL group (12 h light/12 h light) and DD group (12 h dark/12 h dark) were obviously slower than that in LD group (12 h light/12 h dark), mainly showing delayed re-epithelialization, decreased epithelial cells, increased diameter of blood vessel, and delayed re-cruitment of neutrophils and platelets, but more cell number.CONCLUSION:Disruption of circadian rhythms significant-ly inhibits the wound healing of corneal epithelium, mainly through delaying the inflammation and re-epithelialization, but aggravating the inflammatory responses.
6.Biological characteristics of fully demineralized cancellous bone matrix
Qiang LI ; Jicun TANG ; Ruiying WANG ; Chaoyong BEI ; Linwei XIN ; Yayi XIA ; Wenzhong LIU ; Qin ZHANG ; Meng WU
Chinese Journal of Tissue Engineering Research 2009;13(47):9257-9260
BACKGROUND: As an ideal scaffold of cartilage tissue engineering, demineralized bone matrix (DBM) has been widespread used. But some of biological characters remain poorly understood.OBJECTIVE: To determine the degradation capacity, interval porosity and adhesion rate of mesenchymal stem cells (MSCs) onto DBM in vitro.DESIGN, TIME AND SETTING: An observation experiment in vitro was complicated in Institute of Orthopedics, Second Hospital of Lanzhou University from January 8~(th) to April 15~(th) in 2005 and Central Laboratory of Guilin Medical University from August 1~(st) to November 15~(th) in 2007.MATERIALS: One chinchilla rabbit was killed under anesthesia. Referring to the method described by Urist, DBM was made by cancellated bone harvested from metaphysis and vertebral body METHODS: DBM was soaked into phosphate buffered solution to determine its degradation capacity; liquid replacement method was used to test its interval porosity; The 3~(rd) passage MSCs at a concentration of 1×10~8/L were cocultured with DBM in vitro and adhesion rate of MSCs onto DBM was tested using cytometry.MAIN OUTCOME MEASURES: The degradation capacity, interval porosity and adhesion rate of MSCs onto DBM.RESULTS: The degradation rate of DBM was accelerated with the prolonging of time, and the complete degrading time was about 10-12 weeks; The holing rate tested was (77.15±3.44)%; The 3~(rd) passage cells had a higher adhesive rate of 71.25% onto DBM.CONCLUSION: DBM degradation curve is consistent with MSCs proliferation curve, indicating a satisfactory adhesion capacity and interval porosity and DBM is an ideal biological scaffold material for cartilage tissue engineering.