Objective To explore the prominent clinical features and the prognosis of corpus callosum solitary lesions cases in children.Methods There were 16 cases with corpus callosum solitary lesions receiving treatment at our hospital from January to December 2013.All of the clinical features were collected and retrospectively analyzed.Results The 16 cases aged 1 year to 13 years with an average age of (3.44 ± 3.65) years.The causes of the disease included the viral encephalitis,convulsions,and rotavirus infection.The most prevalent disorders were convulsions (10 cases),gastrointestinal symptoms (8 cases),fever (8 cases),positive cervical resistance (6 cases),fatigue (5 cases),altered states of consciousness (2 cases),headache (1 case),and dizziness (1 case).Corpus callosum lesions lasted for 13-50 days,with an average of (28.8 ± 10.2) days.Cranial magnetic resonance showed the splenium of the corpus callosum with isolated oval lesions,low signal on T1 weighted images and apparent diffusion coefficient images,high signal on T2 weighted images and diffusion weighted images.Conclusions The splenium of the corpus callosum lesions of children occur in a lower rate than adults with the etiology including intracranial infection,convulsions and rotavirus infection.Main clinical manifestations include fever,convulsions and gastrointestinal symptoms (nausea,diarrhea,etc).The cases followed-up showed the lesions and symptoms are reversible,and the prognosis is good.

Objective:To detect the anti-cancer component, trans-resveratrol in red wine by high performance liquid chromatography, and to determine the influence of environmental factors on its content. Methods: A method for determining trans-resveratrol in red wine by HPLC is described. The operating conditions were ZORBAX-C18 column(4.6 mm×250 mm, 5μm) at room temperature, 30%acetonitrile as mobile phase at a flow of 1ml/min and UV detection at 306 nm. Results:The content of trans-resveratrol in Changyu dry red wine and Great Wall Claret Cabernet are (1.01±0.03)mg/L and (2.36±0.02)mg/L, respectively. The results show that, sunlight, high temperature and poor seal condition can decrease the content of trans-resveratrol. Conclusion:The content of trans-resveratrol varies in different brands of red wine. Red wine should be stored in cool, dark and sealed conditions.

Objective To explore the usage of anatomic al M-mode echocardiography in observing pulmonary artery motion curve of pulmonary hypertension patients.Methods The subjects included 21 pulmonary hypertension patients complicated with tricuspid valve insufficiency, and 21 controls whose age and gender matched with the patient group. The inner diameter of main pulmonary artery and right atrium, acceleration time of pulmonary valve Doppler signal and maximum velocity of tricuspid insufficiency (ACTpv) were measured during routine echo examination. From the maximum velocity of tricuspid insufficiency the pulmonary artery systolic pressure (PASP) was calculated. The pulmonary artery motion curve and pulsating amplitude of pulmonary artery wall (PAWPA) was observed and measured with a home-made anatomical M-mode echocardiography with instrument. Results PAWPA in the patient group was lower, and simultaneously complicated with broader pulmonary artery, larger right atrium and shorter ACTpv in comparison with normal group (all P

Modern wars result in a significant increase in the incidence of burn injury,and a lot of soldiers with extensive deep burn will occur intensively in a short time.At present,allogeneic skin graft is a principal method to treat extensive burn,but allogeneic skin source is in extremely short supply.Therefore,this review concentrates on the source of allogeneic skin,the necessity to establish allogeneic skin bank for preparedness against war,the present existing problems and main solutions to related problems.

To study the diagnosis of pulmonary stenosis (PS) in patients with the secundum atrial septal defects(ASD) by the measurement of maximal velocity through pulmonary valve (Vpv max ). There were 98 cases with secundum ASD, and 27 cases with secundum ASD and PS diagnosed by echocardiography(TTE). All the cases with secundum ASD were confirmed by surgery. The diagnosis of 19 cases out of 27 cases as diagnosed by TTE were confirmed by surgery, and the other 8 cases were simple secundum ASD. These results suggested that the two dimensional echocardiography and Vpv max should be utilized in order to achieve a better diagnostic accuracy in the diagnosis of PS in the patients with ASD, and PS should be considered if the Vpv max increased to 2 65 to 3 25m/s.

10 h. Results 15 patients had been misdiagnosed as epididymis orchitis or acute abdomen and undergone orchiectomy because of necrosis of the testicles,including 11 cases of intrascrotal torsion (testicular torsion in scrotum with an orchiectomy rate of 79%) and 4 cases of cryptorchidism with torsion.3 cases scrotal orchidopexy has been performed and during the follow up ( 6～36 months) the testicle in 2 of which appeared viable and normal while testicular atrophy has been observed in 1.In recent years 11 adolescents underwent exploration because of acute scrotal pain,7 cases being testicular torsion and 4 acute epididymitis. Conclusions It is emphasized that in younger patients suffering suddenly from pain in scrotum, especially occurring at night,the diagnosis of testicular torsion should be considered.Differentiating cryptorchidism with torsion from incarcerated hernia or acute abdomen,testicular torsion in scrotum from acute testitis and epididymitis should be serious considered.In order to save the testicle with early surgical exploration orchidopexy might be mandatory to aviod recurrence.

Objective To improve the diagnosis and treatment of renal mucinous spindle cell carcinoma,and the knowledge of the type of kidney cancer.Methods The clinical date of 1 case of renal MTSCCa was reported.The case of patient was a 38-year-old women with abdominal discomfort and hematuresis.Ultrasound tip:the upper pole of the right kidney mixed echo placeholder,tumor and the surrounding is obvious blood flow signal.CT revealed a low density shadow in the upper pole of the right kidney,involving the right renal vein,partial filling defect,visible in the inferior vena cava are visible low density shadow,uneven enhancement after the reinforcement,and has delayed reinforcement.MRI shows:the upper pole of the right kidney huge soft tissue mass,T1WI shows low signal,T2WI show slightly higher signal,uneven enhancement and have delayed reinforcement.Preoperative diagnosis:right renal clear cell carcinoma with right renal vein and inferior vena cava tumor emboli.With kidney cancer radical resection and take out inferior vena cava tumor emboli,in the operation right kidney tumor,about 10 cm × 20 cm,right renal vein and inferior vena cava tumor emboli in formation,inferior vena cava tumor emboli within about 8 cm,the clinical and pathologic feature was analyzed and discussed with litcrature review.Results The operation smoothly.Pathological findings suggested higher grade mucinous tubular and spindle cell carcinoma;microscopically a large number of ingredients mixed bundle arrangement of spindle cells,nuclear fission as easy to see.Immunohistochemical staining:Vimentin ±,Syn +,NSE +,CD99 +,Ki-67 + 70％.According to the 2010 AJCC for kidney cancer TNM staging,the pathological pT3c N0M0.According to the Robson stage]].The patient reserved interferonα-2a associate with interleukin-2 theraph after a month.During treatment in patients with fever and other flu symptoms,weekly review of routine blood,liver and kidney function has no obvious abnormalities.The patient died of pulmonary embolism,liver,lung multiple metastases two months later.Conclusions Renal mucinous tubular and spindle cell carcinoma is a rare cell types in the kidney pathological classification,women in high incidence,higher pathological types,worse prognosis.

ObjectiveTo explore the time of chronic spinal cord injury and to determine the time and spatial distribution of glial scar after completely transected rat spinal cord injury. Methods24 adult female Wistar rats were completely transected spinal cord. The responses of activated glial fibrillary acidic protein (GFAP), NG2 and the length of the cavity on the 1st, 3rd, 7th day, 2nd week, 1st, 2nd, 3rd and 3.5th month after operation were examined with histochemistry and immunohistochemistry. ResultsThe length of the cavity and the expression of GFAP begun to be stable at the 2nd month post injury, and were fully stable at 3rd month. The expression of NG2 begun to be stable at the 1st month post injury, and fully stable at 3rd month. ConclusionThe injury begun to stabilize at 1st month post injury, and fully stabilized at 3rd month.

OBJECTIVE To explore the incidence rate,pathogen distribution and characteristics of drug resistance of ventilator-associated pneumonia(VAP) patients due to acute lung injury and acute respiratory distress syndrome(ALI/ARDS) after severe injury,and analyze impact factors on VAP.METHODS All 183 cases with ALI/ARDS after severe injury from Jan 2004 to Mar 2008,were admiitted and given mechanic ventilation over 48 hours in our ICU and EICU.RESULTS VAP was found in 98 cases,accounted for 53.56%.Among them,42 were early-onset VAP and the other 56 were late-onset VAP.Altogether 276 pathogens were isolated,and most of them were Pseudomonas aeruginosa,Staphylococci aureus,Klebsiella pneumoniae,Acinetobacter baumannii and Escherichia coli.In 41 cases only one pathogen was isolated,the other 57 cases were with two or above pathogens.The revealed important risk factors were coma,prolonged mechanical ventilation,tracheotomy,antiacid treatment,use of antimicrobial agents and low albumin.CONCLUSIONS The incidence rate of VAP due to ALI/ARDS is high,and most of the isolated pathogens are drug resistant.Mixed infections are found commonly in these cases.

AIM: To investigate the expression of mitog en -activated protein kinase phosphatase-1 (MKP-1) in cardiac ventricle muscles of spontane ously hypertensive rats and Wistar Kyoto rats at different ages and the relati onship between MKP-1 and myocardial hypertrophy. METHODS: The ratio of left ventricle weight to body weight (LVW/ BW) was measured to reflect myocardial hypertrophy. Protein expression of p-ERK was detected by Western blotting. MKP-1 mRNA in cardiac ventricle muscles was ex amined by RT-PCR. RESULTS: (1) The blood pressure (P