Bone marrow mesenchymal stem cells (BMSCs) is a good seed cell,and it is widely used in intervertebral disc tissue engineering research.The improvement of cellular scaffolds,cell factors and further understanding of intervertebral disc (IVD) cell pathophysiology and BMSCs biology have laid the foundation for cell-based therapies for IVD degeneration.However,BMSCs repairing IVD is still in the early stage of research,at present there are still many problems,one of the most prominent is the survival of transplanted cells.This review mainly introduces the current research status of BMSCs in IVD tissue engineering.

HIFU treatment allows the destruction of a precise volume of tissue within an tumour without harming surrounding structures.The FEP-BY02 model is one of commercially available machines based on this technique,which is proved effectively in clinical practice.It's basic architecture,functional principle and some suggestions for safety use are introduced in this paper.

Medical vacuum knife (MVK) is a new approach for liver surgery. This paper introduces its principles and applications. Some detailed issues related to MVK are also presented such as its differences from other methods, its development and application perspective to abdominal surgery.

Objective To evaluate the formation of coronary collateral circulation with intracoronary Doppler ultrasound in pigs after acute occlusion and the influence of nitroglycerin and phentolamine on it.Methods The blood flow spectrun was detected in 14 pigs using intracoronary Doppler ultrasound before and after acute occlusion of the left circumflex artery (LCX) and its response to nitroglycerin and phentolamine.The average peak velocity (APV) and the diastolic systolic velocity ratio (DSVR) were recorded. Results The APV of the LCX decreased significantly at 10 min after acute occlusion (0.97?1.05 vs 25.78?4.36 cm/s,P0.05). Conclusion Intracoronary Doppler ultrasound can be used to evaluate coronary collateral circulation. The formation collateral circulation was insufficient after acute occlusion of coronary artery in pigs and nitroglycerin or phentolamine can improve the blood flow.

After introducing the predominance and status of remote maintenance,the paper lays emphasis on analyzing its application in medical equipment and problems to be solved in the development.Besides,the developing trend of remote maintenance is forecasted.Remote maintenance is one of the development directions for medical equipment maintenance support.

This paper introduces the applications of network technology, wire and wireless technologies to designs of medical equipments. The theories and applications of such technologies are presented as the ones of serial communication, concurrent communication, USB interface, Bluetooth HomeRF, wireless network communication and GPRS.

Objective To develop a database management system specially applied for medical equipment maintenance administration, Methods Under PowerBuiider/SQL Server development environment, the C/S mode system structure was confirmed and the system function modules were designed. Results The application of C/S mode structure was coincident with the division-cooperation work flow in large and medium hospitals, and the design of function modules met the demand of information collection and utilization of medical equipment maintenance.Conclusion The database management system can preserve the information of medical equipment maintenance effectively and facilitate its usage. Moreover, many statistical indexes about the maintenance administration are available in this system.

Objective: To explore the effect of resveratrol (Res) on angiogenesis of human umbilical vein endothelial cells (HUVEC) with the possible mechanisms in vitro.
Methods: The HUVECs were cultured in 6 groups.①Control group, HUVEC were cultured with high glucose in DMEM,②Res group, the cell were cultured with Res at different concentrations,③Res with PI3K blocker LY294002 (Res+Blocker 1) group, ④Blocker 1 group, HUVEC were cultured with LY294002 alone, ⑤Res with eNOS blocker L-NAME (Res +Blocker 2) group and ⑥Blocker 2 group. The effect of Res on HUVEC proliferation was detected by CCK-8 kit, the protein expressions of p-Akt, p-eNOS were examined by Westin blot analysis, nitric oxide (NO) level was measured by nitrate reduction method and the endothelial cell migration was assayed by transwell chamber method.
Results: ① Compared with Control group, HUVEC proliferation increased in Res (1, 5μmol/L ) group, P<0.01, the proliferation in Res (5μmol/L) group was higher than those in Res (0.2, 10, 20μmol/L) group, P<0.01, while Res (20 μmol/L) group could inhibit the proliferation P<0.01. ②Compared with Control group, Res (5μmol/L) group had the higher protein expressions of p-Akt, p-eNOS, P<0.05-0.01, higher NO level, P<0.05.③Compared with Res group, Res+Blocker 1 group had lower expressions of p-Akt, p-eNOS, P<0.01, lower NO, P<0.05; the expressions of p-Akt, p-eNOS and NO level were similar between Res+Blocker 1 group and Blocker 1 group, all P>0.05.④Compared with Control group, the cell migration and tubing formation were higher in Res (5μmol/L) group, P<0.01;compared with Res group, the cell migration and tubing formation were lower in Res+Block2 group, P<0.01.
Conclusion: Res could up-regulate NO level via activating PI3-K/Akt/eNOS signaling and therefore, improving the proliferation, migration and tubing formation of HUVEC in vitro.

Objective To develop medical equipment management information system for medical engineering department. Methods Under PowerBuilder9.0/SQL and Server2000 development environment, the C/S mode system structure was adopted, and the system function modules, such as data input, browsing, querying, statistical analyzing, report generating, were designed. Results Data exchange between client and server was realized. Meanwhile, the designed function modules achieved normal operation. Conclusion The system structure and functions meet the requirements of working practice in medical engineering department.

Objective To perform multi -condition combination query in the browse window based on PowerBuilder datawindow technology. Methods After the interested combination of query conditions specified on certain columns in datawindow was constructed by if...then statement, the setfilter () and filter () function was used to retrieve and display data which met the filter criteria. Results A flexible query object was incorporated into the data browsing window. Conclusion The datawindow-based query is suitable for situations where batch of data are filtered against not so many columns.