natural enemy. Conclusion Relative humidity, temperature and natural enemy were important ecological factors affecting the population dynamic of T.putrescentiae, and the prevalence curve of T.putrescentiae shows a bimodal pattern.

Objective To study the cryptosporidiosis infection among young children in 29 kindergartens in the province, and to explore the best way to diagnose the infection. Methods Stool specimens of 1 204 children were collected, oocysts of Cryptosporidium were identified with auarmine O-modified staining, acid-fast staining, safranine T and methylene blue staining, and auarmine O-modeified acid-fast staining. Results The detection rate of Cryptosporidium in four stainings were 2.46%,1.50%,1.98% and 3.46% respectively, and the rate was significantly higher by auarmine O-modified acid-fast staining than other stainings (P

Objective To investigate the epidemic situation of trematode Aspidogastrea in the Huaihe River. Methods The mussels in the Huaihe River were collected numbered and dissected to detect the trematode and the species of trematodes were identified after dyeing. Results Totally 79 mussels were collected and the trematode Aspidogastrea were found from 23 mussels with the infection rate of 29.11% 209 trematode Aspidogastrea were collected and the average infectiosity of mussels was 9.09. Conclusion Trematode Aspidogastrea is endemic in the Huaihe River and the species is Aspidogaster sp.

Objective To investigate the acaroid mite pollution in houses in Huaibei area,Anhui province and to prevent allergic diseases caused by acaroid mites. Methods The samples of acaroid mites were collected from the houses,hotels and undergraduate dormitories,then the acaroid mites were separated,classed and counted. Results Fifteen kinds of acaroid mites were detected from the samples,belonging to 5 families,11 genera. The analysis showed that average density of acaroid mites in the common houses ranked the first,32.5. The values of the species richness index R was 1.36,the species diversity index was 2.16,the species evenness index was 0.9. Conclution The density of acaroid mites in the common houses is higher in Huaibei area and the effective measure should be taken to control the acaroid mites.

The influence of acaridida on the peoples' living was discussed in this review, the harm of acaridida to stored grain and human health were mentioned also. It was pointed out that house acaridida pollution was a common phenomenon. So some suggestions were given to control acaridida breeding in house. First of all, make sure that no good living condition for acaridida when you select house and decorate it, secondly, the resistance of furniture to mites should be considered, last but not least, more attention should be paid in daily activity to control house acaridida pollution.

This article discusses 4 methods of deepening reformation entirely to improve quality of running a school in medical college in comprehensive university,such as how to enhance framework adjustment of specialty and construct scientific and reasonable framework of specialty,how to strengthen basic foundation of teaching,improve condition of running a school,how to deepen teaching reformation and reform pattern of students'cultivation,and how to strengthen teaching management and establish system of quality control.All these methods were detailed in this article to offer certain practical experience for other similar medical colleges in comprehensive universities.

Objective To clone,sequence and subclone the cDNA coding for group 1 allergen of Dermatophagoides farinae (Der f 1). Methods The cDNA of Der f 1 was amplified by RT-PCR and PCR. After purified, the gene fragment was cloned into a vector pMD-18T. The recombinant plasmid pMD-18T-Der f 1 was transformed into E.coli JM109. Positive clones were screened and identified by PCR and digestion with restriction enzyme. The sequence of inserted Der f 1 gene fragment was also detected. Der f 1 was then subcloned into the vector of pET-32a(+). Results The Der f 1 gene fragment of Dermatophagoides farinae was specifically amplified from RNA by RT-PCR and PCR. The recombinant plasmid pMD-18T-Der f 1 and pET-32a(+)-Der f 1 was constructed and digested by Bam HⅠand SacⅠ, the size of gene fragment was 646 bp and in accordance with the expected one. Conclusion The pET-32a(+)-Der f 1 subcloning has been constructed successfully.

OBJECTIVE: To establish and optimize the preparation method of protoporphyrin disodium (NAPP) from non-anticoagulant porcine blood.METHODS: Heme was prepared from non-anticoagulant porcine blood firstly.Ferrous sulfide was added into heme to prepare NAPP by saponification and esterification reaction.UV absorption spectrum and infrared spectrum were applied for qualitative test,and ultraviolet spectrophotometry was adopted for quantitative detection of NAPP.Orthogonal experiment was used to optimize the preparation method of NAPP and to determine the optimal parameters of the preparation process.RESULTS: The preparation method of NAPP from non-anticoagulant porcine blood was established.10.6 g heme could be obtained from per kilogram of non-anticoagulant porcine blood and 3.46 g NAPP could be obtained from per 5 g heme.The purity quotient of NAPP was 88.8%.The optimal parameters for the preparation of NAPP were as followed: incremental amount of methanol vs.chloroform was 70 mL ∶ 120 mL in estrification process;Incremental amount of toluene vs.1 mol?L-1 sodium hydroxide-methanol solution was 50 mL ∶ 80 mL in saponification process.The sample was extracted by continuous therma reflux at 90 ℃ for 2.0 h.CONCLUSION: The established preparation method of NAPP from non-anticoagulant porcine blood is an ideal approach for the preparation of NAPP with simple operation,abundant raw material,short preparation cycle,low cost and high yield.

Objective To investigate the composition and diversity of acaroid mites community in stored food in Maanshan area. Methods The samples of 30 kinds of stored food were collected from the supermarket warehouse and storehouse in July, 2006. The acaroid mites were isolated by Waterflotation and Tullgren, identified and counted under the microscope, the composition and diversity of acaroid mites community were analyzed. Results 15 species of acaroid mites which belong to 7 families and 12 genera were found. The average breeding density of acaroid mites was 3.56/g and the species richness index was 1.63, the diversity index was 2.28, the evenness index was 0.84. Conclusion The composition of acaroid mites community in stored food takes on diversification and the polluted situation is serious, vacuum packaging is an effective methods to decrease mites breeding.

Objective To investigate the composition and diversity of acaroid mite community in the house in Xuancheng area in Anhui province.Methods The dust samples from 4 sites selected in each house were collected in June-July,2006,acaroid mites were isolated,counted and identified.Results 3 386 acaroid mites were obtained,belong to 6 families,12 genera and 14 species.The species richness index Rmargalef for the four habitats ranged from 0.73 to 1.75,while the species diversity index(Shannon-Wiener index) ranged from 1.36 to 1.87 and Pielou index ranged from 0.70 to 0.92.Conclusion The results of the present paper show that the habitat condition is the main factor that can directly influence the community structure and diversity of acaroid mites in the houses and the human activity is also an influencing factor to be reckoned with.