Objective To make a concrete analysis of micro-perfusion pump and compatible syringes used in neonatal intensive care unit (NICU) for low birth weight infants,and to find solutions to the defects in application.Methods 20 ml sterile syringes were set different infusion rates (1ml/h,5ml/h,6ml/h,10ml/h),different infusion time (5min,30min,lh).Then,the card slot on the micro-perfusion pump where the end of 20ml sterile syringe was placed was regulated.The regulated syringes were also set different infusion rates (5ml/h,6ml/h,l0ml/h) and time as before.The error accuracy was calculated.Results The results showed that at the end stage of 20ml sterile syringes pumping,there was always 0.5～1.5 ml residual liquid which could not be pumped out,but perfusion pump displayed injection was completed.Before regulation,the error accuracy between different infusion rates of 20ml sterile syringes was 6.00％,3.80％,2.33％,1.30％ respectively.However,after improvement,the regulated syringes could completely pump all liquid out,and the error accuracy between different infusion rates and different infusion time was also superior to pre-improvement.Conclusions Improved micro-infusion pump and sterile solution prefilled syringe have no drug residual,and display high accuracy and small error accuracy.

OBJECTIVE To evaluate the quality of Indigo Naturalis， and to provide reference for the quality control of Indigo Naturalis. METHODS UPLC-MS/MS method was used to determine the contents of 6 indole alkaloids （indigo， indirubin， isatin， tryptanthrin， indole and indole-3-carboxaldehyde） in Indigo Naturalis from different origins. Cluster analysis， principal component analysis and partial least squares-discriminant analysis （PLS-DA） were used to evaluate the quality of Indigo Naturalis from different origins. RESULTS The contents of indigo， indirubin， isatin， tryptanthrin， indole and indole-3-carboxaldehyde in Indigo Naturalis from different origins were 20 320.83-26 585.01， 1 327.69-3 102.25， 141.69-894.50， 2.17-5.27， 2.14-5.93 and 1.69-4.34 μg/g， respectively. The Indigo Naturalis from different areas were clustered into two categories by cluster analysis. Samples S1， S2， S4， S6， S7， S9 and S10 were clustered into category Ⅰ， and samples S3， S5， S8， S11 and S12 were clustered into category Ⅱ. Indigo Naturalis from different origins was evaluated with 3 principal components. The results showed that category Ⅰ sample scored higher and had better quality， while category Ⅱ sample scored lower and had worse quality. PLS-DA showed that indigo， indirubin， tryptanthrin and isatin were the main substances that reflected the quality difference of Indigo Naturalis. CONCLUSIONS The quality of Indigo Naturalis from different origins is different， and the quality of Indigo Naturalis of different batches from the same area is not stable. The quality evaluation method of Indigo Naturalis established in this paper is stable and reliable， which can provide a basis for the quality control of Indigo Naturalis.