Objective To investigate the effect of Hongjintian decoction on the levels of serum MDA , SOD, NGF, MBP and bFGF of physical fatigue athletes.Methods 40 students of physical education college were randomly divided into experimental group and control group, 20 cases in each group.After the end of sport, the experimental group were given Hongjintian decoction, and control group were given sugar water, the levels of serum MDA, SOD, NGF, MBP and BFGF in two groups were detected.ResuIts Compared with control group, the level of MDA in experimental group was lower(P<0.05), and the level of SOD was higher (P<0.05); NGF and BFGF levels were higher in experimental group(P<0.05), MBP level was lower (P<0.05).ConcIusion Hongjintian decoction can significantly reduce serum MDA, MBP levels, increase serum SOD, NGF and BFGF levels of physical fatigue athletes.

Dengue is the most wide-spread arthropod-borne viral disease of humans in the tropic and sub-tropic regions.In this study,genomic sequences of more than 3 000 dengue viruses available in the GenBank were aligned and analyzed by sero type.According to phylogenetic trees generated by the minimum evolution method of MEGA5.0,dengue viruses were divided into 4-6 genotypes within the four serotypes,respectively.Meanwhile,it was indicated that the distribution of most genotypes was associated with geographic origins of dengue viruses.Probable origins for most of the 39 strains from China with genomic sequences were deduced from relevant ancestral strains in the context of ME trees.These results revealed that the genotype distribution of dengue viruses was geographic origin-specific at genomic level,and that diverse introduction sources were attributed to dengue outbreaks in China.

A novel automatic manufacture device for tissue micro-array is introduced in this paper. Based on the analyses of task and process, the new device prototype is researched and developed. The device consists of a paraffin positioning module and a three-manipulator module. The control system is composed of accurate navigation sub-system, digital image recognition sub-system and punching-filling operating sub-system. The results of experiment demonstrate that the device can accomplish the operations such as image automatic recognition, accurate position, auto-punching and filling. It fulfills the requirements to automatic manufacture of tissue micro-array.
Automation ; Equipment Design ; Humans ; Tissue Array Analysis ; instrumentation ; methods

This study used the natural flavonoid compound silybin as the parent compound and synthesized 16 silybin derivatives through oxidative dehydrogenation, alkylation, selective demethylation, and acylation. The structures of these derivatives were confirmed by 1H NMR, 13C NMR, and MS. All derivatives were found to be new compounds never reported in previous literature. Using gastric cancer cell line SGC-7901 and human glioblastoma cell line LN-229, the in vitro anti-tumor activity of the novel silybin derivative was determined through MTT assay with lapatinib as the positive control. The experimental results indicate that the synthesized novel silybin derivatives have a certain degree of anti-proliferative effect on two types of cancer cells, with compounds I2 and I14 showing strong anti-proliferative activity against LN-229 and SGC-7901 cells.

OBJECTIVETo analyze the risk factors of surgical site infection (SSI) following posterior lumbar intervertebral fusion.

METHODSThis retrospective case-control study was conducted in 2904 patients undergoing posterior lumbar intervertebral fusion from 2011 to 2016. Forty-three patients with SSI within 30 days after the operation served as the case group, and 334 randomly selected patients without infection served as the control group. Age, gender, diabetes, body mass index (BMI), albumin level, multilevel procedures, subcutaneous fat thickness, surgery duration and the percentage of lumbar multifidus muscle fat infiltration were analyzed, and univariate and multivariate logistic regression analyses were performed to identify the risk factors of SSI.

RESULTSMultivariate logical regression analysis identified a female gender, subcutaneous fat thickness, multilevel surgery, and lumbar multifidus muscle fat infiltration as significant risk factors for SSI ( < 0.05). BMI was not correlated with fat infiltration in the lumbar multifidus muscle ( > 0.05).

CONCLUSIONSA female gender, multilevel surgery, subcutaneous fat thickness and fat infiltration in the multifidus muscle are related to SSI following posterior lumbar intervertebral fusion. Fat infiltration in the multifidus muscle was a spine-specific risk factor for SSI independent of BMI.