0.05) after non-fever limit (non-FL) dose and FL dose endotoxin (ET) was intravenously injected into rabbits. The increase of PGE_2 in CSF was not limited during the occurrence of ET FL. 2. Intracerebroventricular injection (ICV) of different dose of PGE_2 into rabbits induced dose-dependent fever, but there was no more rise in body temperature when the febrile response had reached a certain height. This is termed "PGE FL". 3. The concentration of cyclic adenosine-3′, 5′-monophosphate (cAMP)in CSF paralleled the fluctuation of temperature (r=0.9906, P

40 New Zealand white rabbits were used to observe the effect on the fever caused by ET (endotoxin) and EGTA [ethylene glycol bis-(?-aminoethylethylether)-N, N, N′, N′-tetraacetic acid] after ICV (intracerebroventricular) infusion of Tau and CaCl_2 into rabbits. The results showed that Tau ICV perfusion could inhibit the initial febrile response to ET and EGTA in rabbits (P0.05), which could be blocked by ICV infusion of CaCl_2. The authors suggested that Tau might increase the level of Ca~(++) in the hypothalamus and reduce Na~+/Ca~(++) ratio, then lower the fever.

Objective To examine miRNA-31 expression in serum of children with airway allergic disease (asthma and allergic rhinitis) and determine the correlation with CD44.Methods The expression of miR-NA-31 and CD44 in serum of 26 children with acute asthma and allergic rhinitis and non-acute phase and 36 normal control children were determined by real-time reverse transcriptase polymerase chain reaction.Results The miRNA-31 was significantly overexpressed in serum of children with acute asthma and allergic rhinitis compared with non-acute phase and normal control children and significantly correlated with CD44.Conclusion The correlation between miRNA-31 and CD44, the aberrant expression of miRNA-31 may account for the occurrence and development of airway allergic diseases.

Objective To investigate neoplastic lesions in gastrointestinal tract treated by endoscop-ic mucosal resection (EMR). Methods The properties, extent and infiltrating depth of the neoplastic lesions in gastrointestinal tract were detected by chromoendoscopy, magnifying videoscopy and endoscopic ul-trasonograpy. The tumors in niucosa or its muscular layer including the early cancer, precancerous lesion, submucosal tumors, polyps in laterally development, large polyps with or without peduncle were removed by EMR or transparent plastic cap EMR. Results Thirty-one submucosal tumors and four early cancers (esoph-ageal cancer 2, cardiac carcinoma 1 , early colonic carcinoma 1 ) and moderate or severe gastric dysplasia 2 were treated by EMR or transparent plastic cap EMR, no recurrence happened within 3-18 months follow-up. Laterally spreaded 13 polyps and 21 subpedunculated or sessile typed large polyps were resected by the procedures of EMR and Endoscopic Piecemeal Mucosal Resection ( EPMR). Five (7. 04% ) cases were complicated with bleeding during the procedure, and cured under endoscopies. One patient with villus adenoma ( 1. 41% ) recurred three months after EMR. Conclusion Under the guidance of chromoendoscopy, magnifying videoscope and endoscopic ultrasoundgraphy, EMR is a safe and effective endoscopic therapy for early cancers, submucosal tumors and large polyps in gastrointestinal tract.

Objective To evaluate the efficacy and safety of sodium aescinate for abdominal operation in general surgery.Methods A multicenter,prospective and open labeled trail was conducted.Data of 2001 patients in PPS group who had undergone either conventional treated (n =500) or sodium aescinate treated (n =1 501) for abdominal operation in general surgery were analyzed.In the sodium aescinate treated group,sodium aescinate was given by ivgtt 12 h before the surgery started,and 20 mg daily after the surgery for 6 days.Time to recovery of gastrointestinal sounds (TRGS),time to recovery of passage of gas (TRPG),and time to recovery of bowel movements (TRBM) were recorded to evaluate the bowl function recovery.The difference of incision edema was observed by the evaluate edema level.Results The TRGS of sodium aescinate treated group was significantly shorter than that of the conventional treated group,P ＜ 0.01.The TRPG of sodium aescinate treated group was significantly shorter than that of the conventional treated group,P ＜ 0.01.The TRBM of sodium aescinate treated group was significantly shorter than that of the conventional treated group,P ＜ 0.01.The incision edema of sodium aescinate treated group was better than that of conventional treated group.The rate of adverse event was low in the sodium aescinate treated group,phlebitis was the most common one,accounting for 0.66％.No serious adverse event happened.Conclusion Sodium aescinate is effective and safe in accelerating patients recovery in general surgery.

AIM: To identify and quantitative analysis gentiopicroside content in the extract of Violent gentian of Tibetan herbal medicine. METHODS: Application of HPLC-the electricity sprays fog-mass spectroscopy/mass spectroscopy(HPLC-ESI-MS/MS) and the technique of LC-DAD-UV,by contrast with the reservation time of the authentic gentiopicroside,the ultraviolet absorbed spectrum,the molecule~+,quickly identified the gentiopicroside in the extract of violent gentian.As to it's quantitative analyis we adopted the capillary HPLC methods,with the weight of authentic gentiopicroside for abscissa. RESULTS: MS showed that the methanol extract of violet gentian mainly had five peaks.the compound structure of first peak was gentiopicroside,molecule~+ 356.9,result showed that gentiopicroside in gentian was 1.97%. CONCLUSION: Gentiopicroside is the mainly component of violet Gentia.

AIM: To investigate association between children interleukin 1?(-511) gene polymorphisms and susceptibility to febrile seizures. METHODS: Children were devided into two groups: those with febrile seizures (FS) and healthy control, FS group was also devided into family history group and non-family history group. Single nucleotide polymorphisms of IL-1?-511 gene in promoters were detected by PCR-RFLP among these groups. RESULTS: Genotypes for IL-1? promoter -511 (T-T, T-C, C-C) in total febrile seizures and healthy controls were not significantly differen. Genotype IL-1?-511(T-T) in family history patients is 46.2% ,which is much higher than those in non-family history group (10.3%) and healthy control group (5.7%). The genotype proportions for IL-1? promoter-511 in family history group were markedly different from those in non-family history patient group and control group (P

Based on Sun Yat-Sen University Cancer Hospital’s practice of applying big data for prevention of registration traffickers,the paper analyzes the behavioral characteristics of technology-based registration traffickers'malicious occupation from the aspects of abnormal registration,abnormal withdrawal,abnormal grab and abnormal user binding,makes the corresponding technical plans and countermeasures,so as to limit the malicious registration of registration traffickers using the reservation system and guarantee the medical resources can be distributed to patients fairly and impartially.

Objective: To study the optimum extraction condition of effective composition in Rhizoma Alpiniae Officinarum by CO 2 supercritical fluid extraction. Methods: CO 2 supercritical fluid extraction condition was selected by uniform design. The extraction pressure, extraction temperature, analytic pressure, analytic temperature were four factors in the experiment. Seven levels of each factor were selected.Results: The optimum extraction condition in SFE was: extraction pressure at 30MPa, extraction temperature at 32?C , analytic pressure at 6MPa, analytic temperature at 36?C . Conclusion: The method is simple, selective and efficient.

Objective To investigate the effect of hepatitis C virus (HCV) genotype on antiviral therapy in patients with human immunodeficiency virus (HIV)/HCV coinfection in Henan province.Methods A total of 129 patients were coinfected with HIV and HCV, among whom, 70 were HCV 1b genotype and 57 HCV 2a genotype.And 131 patients were HIV single infection.Immunological failure rate, virological suppression, CD4+ T lymphocyte counts and liver and renal function after antiretroviral therapy (ART) were compared among the three groups.Flow cytometry was used to count CD4+ T lymphocytes and polymerase chain reaction amplification was used to detect HIV RNA.The liver and renal function were tested by automatic biochemical analysis.Statistical analysis was conducted by χ2 test, analysis of variance and LSD-t method.ResultsImmunological failure rate in HCV 1b group, HCV 2a group and HIV single infection group were 7.14% (5/70), 15.79% (9/57) and 9.92% (13/131), respectively.There was no significant statistical difference among the three groups (χ2=2.59, P>0.05).The CD4+ T lymphocyte counts in three groups were (614±258), (529±245), and (518±243) cells/μL, respectively.The difference was statistically significant (F=3.17, P<0.05).The virus inhibition rates of three groups were 87.0% (HCV 1b), 78.2% (HCV 2a), and 82.3% (HIV single infection).The HIV virus failure rates were 8.6% (HCV 1b), 14.5% (HCV 2a), and 13.1% (HIV single infection).There was no significant difference among three groups (χ2=1.967, P>0.05).The levels of aspartate transaminase, alanine aminotransferase and total bilirubin in HCV 1b group and HCV 2a group were all significantly higher than those in HIV single infection group (F=27.38, 15.22 and 7.33, respectively, all P<0.05), while there was no significant difference between HCV 1b and HCV 2a groups (t=1.27, 0.29 and 1.59, respectively, all P>0.05).Conclusions The main HCV genotypes in patients with HIV/HCV coinfection by blood transmission are HCV 1b and HCV 2a in Henan province.HIV/HCV coinfection does not affect the effect of ART, but could aggravate the liver damage in acquired immune deficiency syndrome patients.