Based on clinical features of refractory diseases and clinical practice experience for many years,the author expounded the therapeutic strategies from establishing individualized system of differentiation treatment,treatment strategies of refractory diseases,and the key for medication,and emphasized individual concerned therapy,separating primary and secondary treatment and grasping the physiological state to treat pathological changes.Thus complicated and changeable diseases can be treated clinically.

Microorganisms can affect the development of body immune system.Intestinal flora regulate immune function by promoting the intestinal immune system development,inducing T cell differentiation to avoid or reduce the incidence of immune-related diseases.Allergic diseases are related with the body's immune system hypoplasia and imperfect immune regulation mechanism.The immune system can be affected by intestinal flora,and the distribution of flora in the body between children with allergies and healthy children are significantly different,indicating that the occurrence of allergic diseases are associated with intestinal microflora.Studies have reported probiotic are helpful to the prevention and treatment of allergic disease,providing a new way for the prevention and treatment of allergic diseases.

Anti-Bacterial Agents ; therapeutic use ; Emergencies ; Enterocolitis, Necrotizing ; diagnosis ; epidemiology ; etiology ; prevention & control ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; diagnosis ; epidemiology ; etiology ; prevention & control ; Infant, Very Low Birth Weight ; Infection Control ; Intensive Care Units, Neonatal ; Premature Birth ; prevention & control ; Risk Factors

Brain Injuries ; surgery ; Female ; Humans ; Hypoxia-Ischemia, Brain ; surgery ; Infant, Newborn ; Male ; Neural Stem Cells ; cytology ; transplantation

Lin28 is a highly conservative RNA-binding protein. It can bind to the terminal loop of the precursor of let-7 family miRNAs and block the maturation of let-7. Lin28/let-7 signal pathway is involved in the stem cell pluripotency and tumor development. Combined transfection of Lin28, OCT4, SOX2, and NANOG can reprogram human somatic cells into pluripotent stem cells. Tumors with high expression of Lin28/Lin28B are often poorly differentiated and have poor prognoses.

Objective: To observe the influence of hepatocyte growth-promoting factor (pHGF) on monocyte chemotatic protein-1-(MCP-1) and aristolochic acid I (AA I )-induced epithelial-to-mesenchymal transition (EMT) and apoptosis of human kidney epithelial cell line(HKC). Methods: The HKC cells were randomly divided into blank control group (control groups), epithelial-to-mesenchymal transition model group (model group), and pHGF inhibition group (pHGF groups) with pHGF at different concentrations (0. 15, 1. 5, 15, 150, and 1 500 ng/ml). The EMT model was established by exposing HKC cells to MCP-1 (0. 1 μg/ml)and AA I (10 μg/ml). Cells in the pHGF groups were the model cells treated with different concentrations of pHGF. Cells in the control group were cultured routinely. WST-8 method and flow cytometry were used to observe the proliferation and apoptosis of HKC cells, respectively. The mRNA expression of α-smooth muscle actin(α-SMA) was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), and the expression of α-SMA, fibronectin (FN), and transforming growth factor-β1 (TGF-β1) in HKC cells were assessed by indirect enzyme immunohistochemistry. Results: The cell inhibitory rate, apoptotic rate, and expression of α-SMA mRNA were significantly increased in the model group and pHGF groups compared with those in the control group (P<0. 01), indicating the successful establishment of EMT model. Compared with the model group, pHGF at 150 ng/ml, but not at other concentrations, significantly decreased the inhibition rate of HKC cells(P<0. 01). The apoptotic rate of HKC cells in all the pHGF groups were significantly lower than that in the model group (P<0. 01), pHGF at 150 ng/ml also greatly decreased the expression of α-SMA mRNA, and significantly down-regulated the expression of α-SMA, TGF-β1, and FN protein. Conclusion: pHGF at 150 ng/ml can partly reverse MCP-1- and AA I -induced HKC cell growth inhibition, apoptosis, and EMT.

3cm than in the patients with the largerest dominant diameter≤3cm.Embolization with combination of PLE and GSP had improved the percentage of reduc- tion in myomas volume.Conclusion The factors affecting results include tumor size and location,using of GSP,and performing of bilateral embolization.

Histamine H 1R antagonists are mainly administered to treat the diseases of hypersensitivity reaction. And histamine H 2R antagonists are mainly administered to treat gastroenteric diseases. But in recent years administered simultaneously H 1R and H 2R antagonists can enhance their effects. Combination of H 1R and H 2R antagonists has good therapeutic effect on hypersensitivity reaction, cancers, asthma, etc, and can eliminate side effects.

AIM: To evaluate the efficacy and safety of multiple vitamin E, C, B facial mask for the patients with acne vulgaris. METHODS: One hundred and seventeen patients aged over 13 a, with acnes mainly sited on the face and an acne severity rating above grade 2 were enrolled in this study. The mask treatment was used once a day with 8 wk as a course.The primary endpoint included the number and the degree of acne lesions including comedoes, papules, pustules and cysts. The secondary endpoint was the overall efficacy evaluation by the physicians. RESULTS:After 8 wk of treatment with multiple vitamin E, C, B facial mask, the mean number of acne lesions:comedoes, papules, pustules and cysts decreased 13 + s 13,10 ± 12, 5 + 7 and 3 + 3, respectively. These changes were statistically significant (P ＜ 0.01 ). The results of overall efficacy evaluation showed that 7 patients '(6.0 % ) symptom was greatly improved, 44 patients '(37.6 % ) symptoms were moderately improved, 56patients'(47.9 % ) symptoms were slightly improved,and 10 patients' symptoms did not change. Among all 117 patients, 59 patients (50.4 % ) showed no adverse reactions, including swelling, redness, itching,burning or scaling. CONCLUSION: Multiple vitamin E, C, B facial mask is efficacious for the treatment of acne and more than half study subjects experienced in none adverse reaction. Multiple vitamin E, C, B facial mask possesses an alternate therapy in acne vulgaris.

Anemia of prematurity is very common in preterm infant.Phlebotomy losses and inadequate production of erythropoietin are the main reasons.Treatment of anemia of prematurity includes delayed umbilical cord clamping at delivery,decreasing phlebotomy losses,use of red cell growth factors such as erythropoietin properly,red blood cell transfusions,which are based on indications and guidelines.In this article we review recent studies in preventing and treating anemia in preterm infants.