Acupuncture Therapy ; Adolescent ; Adult ; Bone Diseases ; immunology ; therapy ; Cartilage ; Cartilage Diseases ; immunology ; therapy ; Female ; Humans ; Male ; Ribs ; immunology ; Young Adult

Brain Injuries ; surgery ; Female ; Humans ; Hypoxia-Ischemia, Brain ; surgery ; Infant, Newborn ; Male ; Neural Stem Cells ; cytology ; transplantation

Objective:To explore the effect of gene hepaCAM transfection on the proliferation in human bladder carcinoma cell line. Methods: Plasmid pEGFP-N2-hepaCAM was transfected into T24 cells by Lipofectamine2000, and the T24 cell stably expressing hepaCAM gene was established by G4l8 selection.The expression of hepaCAM mRNA and protein was detected by RT-PCR and Western blot assays. The effects of hepaCAM on cell proliferation were measured by MTT and colony formation assays. The cell cycle progression were measured by flow cytometry. The expression of CyclinD1 mRNA was detected by RT-PCR. Results:Expression of hepaCAM gene and protein were proved by RT-PCR and Western blot assays. The MTT assay showed cell growth in the T24/hepaCAM-GFP group was significantly slow when compared with non-transfection and mock-vehicle groups whereas the rates of clonal formation were greatly decreased(P

[Objective]For more pertinently opening medical students mental health education course.[Methods]Through the issuance of 500 copies of ques-tionnaires, the current medical kind of col ege students' psychological health education curriculum requirements and attitude is researched. [Result] The medical specialty of college students ' psychological health status, with a certain degree of self adjustment ability, as wel as improvements to the curriculum and teaching methods are expected. [Conclusion]Recommended that through played curriculum, scientific curriculum, to strengthen the course construc-tion of improvements in areas such as mental health course.

Objective To explore the expression and significance of vascular endothelial growth factor (VEGF),tissue polypeptide specificity antigen (TPS) and tumor type M2 pyruvate kinase (TuM2-PK) before and after interventional therapy in lung cancer (LC).Methods Sixty cases of LC treated by interventional treatment in our hospital from January 2013 to January 2015 were analyzed.The levels of VEGF,TPS and TuM2-PK on preoperative 1 d,postoperative 1,3,7,14,28 d were detected by enzyme-linked immunosorbent assay(ELISA).The change of VEGF,TPS and TuM2-PK levels in the patients with different treatment outcomes was observed.Contemporaneous 40 individuals undergoing healthy physical examination served as the healthy control group.Results The levels of VEGF,TPS,TuM2-PK on preoperative 1d,postoperative 3,7,14,28 d had statistical differences between the observation group and control group (P<0.05,P<0.01).Compared with pre-treatment,the levels of VEGF,TPS and TuM2-PK in partial remission patients were significantly improved,which in the patients with stable and progressive condition,the levels of VEGF,TPS and TuM2-PK were significantly increased (P<0.05).Conclusion The levels of VEGF,TPS and TuM2-PK are significantly elevated before and after interventional treatment in LC patients,moreover the worse the therapeutic efficacy,the higher the levels of VEGF,TPS and TuM2-PK,which can be used as the detection indexes of LC interventional treatment.

Objective To investigate the risk factors and microbial causes related to the pathogenesis of ventilator-associated pneumonia(VAP).Methods Totaly 138 intubated newborn infants for longer than 48 hours in Ningbo Women and Children's Hospital were studied,who were divided into VAP group (56 cases)and non VAP group(82 cases).Microbial causes of VAP were analyzed.Results Malnutrition,repeated tracheal intubation,repeated endotracheal suctioning,indwelling tube,posture,hyaline membrane disease,gestational age,birth weight,duration of intubation,duration of hospitalization were independent risk factors for ventilator-associated pneumonia(P<0.05).Klebsiella pneumoniae,pseudomonas aeruginosa,acinetobacter species and enterobacter cloacae were the main pathogenic bacteria of VAP.Conclusion Shortening of mechanical ventilation duration,avoiding repeated tracheal intubation and indwelling tube usage,semi-fowler positioning can lower the incidence of VAP.Pathogenic bacteria control is essential for VAP.

Objective To explore the diagnosis and treatment for the incision cesarean section scar pregnancy(CSP).Methods The clinical data of 34 patients with CSP admitted were retrospectively analyzed,and analyzed the methods of diagnosis and treatment of CSP.Results Our results showed that 9 cases after treatment with mifepristone combined with suction palace obtained success,2 cases of bleeding assisted with balloon compression;8 cases after mifepristone combined hysteroscopy under suction palace treatment obtained success,1 case was failed and transfer to laparotomy.Mifepristone combined with transvaginal lesion resection of scar pregnancy was succeed in 9 cases,2 cases were failed,of which 1 case was bleeding more and transfer to laparotomy,1 case was confirmed remaining during a review after half a month open.7 cases were succeed in the transabdominal lesion resection of pregnancy (3 cases of transit);without hysterectomy;3 cases were turn to the superior hospital for treatment of intervention.The statistical results showed that no statistical significant difference was obtained among the scores of different age, length of hospital stay, hospital expenses and rescue of shock in different teams of surgery.For the haemorrhage amount, the bleeding was at least in hysteroscopy group, and was at most in abdominal resection of lesions group (rescue of shock was on the high side).Conclusion Uterine scar pregnancy is a relatively rare and dangerous clinical special type of ectopic pregnancy, early diagnosis and appropriate therapeutic strategy are important.The mifepristone combined with suction palace and resection of the pregnancy lesions are safe and effective method for treatment.

Objective To investigate the effect of electroacupuncture plus functional exercise in patients with olecranon comminuted fracture.Method Seventy-sevenpatients with olecranon comminuted fracture were enrolled as the subjects and allocated, using a random numbertable, to a control group (38cases) and a treatment group (39 cases).The control group received routine rehabilitation such as postoperative functional exercise and the treatment group, electroacupuncture in addition.Inthe two groups of patients, the pain score was recorded at one,sevenand fourteen days after the surgery; elbow joint activity was examined at two days and two months after the surgery; the elbow function score was recorded at six months after the surgery.Result There was no statistically significant difference in the pain score between the twogroupsat one day after the surgery (P>0.05).Thepain score was lower in the treatment group than in the control group atsevenand fourteen days after the surgery (P<0.05).There was no statistically significant difference in elbow jointactivity between the two groupsat two days after the surgery (P>0.05). Elbow joint activity was larger in the treatment group than in the control group at two months after the surgery (P<0.05).The elbow HSS score was higher in the treatment group than in the control group at six months after the surgery (P<0.05).Conclusion Electroacupuncture plus functional exercise can effectively decrease the pain score and increase elbow joint activity and the elbow function score in patients with olecranon comminuted fracture.It has greater value in clinical application.

15 cases of diaphragmatic traumatic hernia were reported. The majority of patients were young people. The location of lesion was on the left in 14 cases and one on the right. There were 8 close and 7 open cases. The size of the rupture on the diaphragm was from 3 to 15cm. The importance of earlier diagnosis and treatment were also discussed.

The second part of (2008 edition)includes iodine deficiency disease,thyroditis and thyroid nodule.This guideline reaffirms the epidemic characteristics,the prevention and the management of iodine deficiency disease.It identifies the measurement of iodine nutritional status and the necessity of iodinized salt.It emphasizes the regular diagnosis and treatment of subacute and chronic thyroiditis,and also mentions the advancement on differential diagnosis and rational therapy of thyroid nodules.In addition,it gives some advices on the management of thyroid nodules in two special groups(pregnant women and children).