Objective To observe the effects of cisplatin on the expression of tumor stem cell markers in human hepatoma cell line HepG2 .Methods The cultured human hepatoma HepG2 cells in logarithmic growth phase were used for the study ,which were divided into control(no special treatment was administrated) and experimental groups(cisplatin was added) ,then the rates of cell growth inhibition ,transitional healing rates and changes in tumor associated markers associated P53 ,caspase‐3 ,caspase‐8 ,CD133 ,a‐denosine triphosphate‐binding cassette superfamily G member 2(ABCG2) ,intercellular cell adhesion molecule‐1(ICAM‐1) were measured .Results Comparison of cell growth inhibition :with the increase of drug concentration increased ,compared with the con‐trol group ,the difference was statistically significant(P<0 .05) .Transitional healing rate:with the increase of drug concentration , compared with the control group ,the difference was statistically significant(P< 0 .05) .The expression of related tumor markers P53 ,caspase‐3 ,caspase‐8 increased while the drug concentrations increase and were higher in the control group(P< 0 .05) ,the difference were statistically significant .In the immunofluorescence for the detection of CD133 ,ABCG2 ,ICAM‐1 ,the fluorescence in‐tensity increased with the concentrations of cisplatin .Conclusion Cisplatin could inhibit the growth of tumor cells by up‐regulating the tumor markers such as P53 ,caspase‐3 and caspase‐8 .The migration of tumor cells in cell scratch test could also be inhibited by cisplatin w hile their drug resistance w ere enhanced .

Objective Linkage analysis were performed in 2 pure Chinese paroxysmal kinesigenic dyskinesia families to localize the locus of them. Method Microsatellites markers corresponding to pericentrometric region of chromosome 16 were used in parametric and nonparametrie linkage analysis for 27 members in the 2 pedigrees, haplotypes were constructed subsequently. Result The maximum LOD score and NPL score in the 2 families were all negative, P values were significantly larger than 0.05.No haplotype segregated with PKD phenotype was found. It showed no evidence of association with known PKD loci in both pedigrees, providing evidence for a novel PKD locus. Conclusion PKD is heterogeneous, a novel PKD locus may be in pure Chinese pedigrees.

Hospice care is a health care involving end-of-life quality of millions of people. Compared with the growing demand for hospice services, the domestic service supply is seriously insufficient, making it urgent to explore the development path to meet the diversified health service needs of the public. Based on the analysis of the current situation of hospice development in the mainland and Taiwan of China, the " five-in-one" hospice development path consisting of top-level design, legislative protection, personnel training, social support, and publicity and education was proposed, which was expected to provide a basis for promoting the development of hospice practice in Chinese mainland. The aim is to provide a reference for promoting the development of hospice practice in Chinese mainland.

Objective:Given the indispensable role played by medical ethics committees in protecting the rights and interests of subjects and researchers, such committees in China are found with weak organizational capacity. This study was designed to analyze the driving factors to improve their organizational capacity.Methods:Form July to September 2020, a survey and interview of 71 tertiary A hospitals in eastern China were conducd, and a total 60 valid samples were obtained. Through crisp-se qualitative comparative analysis(csQCA), the medical ethics committees of 60 tertiary hospitals were analyzed, and the factors affecting the organizational capabilities of the medical ethics committees and their combinations were explored.Results:Protection of rights and interests was key to medical ethics committee′s organizational capacity, while incompetency of the members and the irregular review process were sufficient reasons for the weak organizational capacity. There were six combinations of sufficient conditions for the medical ethics committee to be strong in organizational capacities, which could be explained by the three models of member capacity-orientation, system-process-orientation, and resource-system-orientation.Conclusions:The medical ethics committees are recommended to enhance members capacity, enhance team building; complete rules and regulations, and process mechanism; clarify organization structure, and optimize resource matching, in an effort to enhance the organizational capacity of these committees in China.

The widespread application of next generation sequencing (NGS) in clinical settings has enabled testing, diagnosis, treatment and prevention of genetic diseases. However, many issues have arisen in the meanwhile. One of the most pressing issues is the lack of standards for reporting genetic test results across different service providers. The First Forum on Standards and Specifications for Clinical Genetic Testing was held to address the issue in Shenzhen, China, on October 28, 2017. Participants, including geneticists, clinicians, and representatives of genetic testing service providers, discussed problems of clinical genetic testing services across in China and shared opinions on principles, challenges, and standards for reporting clinical genetic test results. Here we summarize expert opinions presented at the seminar and report the consensus, which will serve as a basis for the development of standards and guidelines for reporting of clinical genetic testing results, in order to promote the standardization and regulation of genetic testing services in China.