Objective: To explore the characteristics of new occupational diseases in Changzhou City from 2006 to 2018, and provide scientific basis for relevant departments to formulate prevention and control strategies. Methods: In July 2019, the data of new occupational diseases in Changzhou City from 2006 to 2018 were collected, and the distribution of new occupational diseases, such as disease types, regions, population, industries, economic types and scale of enterprises were analyzed. Results: From 2006 to 2018, a total of 432 new cases of occupational diseases were reported in Changzhou, mainly including occupational pneumoconiosis and other respiratory diseases (175 cases, 40.51%) , occupational chemical poisoning (130 cases, 30.09%) , and occupational otorhinolaryngological and oral diseases (83 cases, 19.21%) . There were 378 males (87.50%) and 54 females (12.50%) . The gender distribution of new occupational diseases in different categories was statistically significant (χ²=32.12, P<0.01) . The average age of onset was (48.3±12.2) years, of which 40-49 years (152 cases, 35.19%) were the most. The age distribution of new occupational diseases in different categories was statistically significant (χ²=157.53, P<0.01) . The average length of service was (9.38±9.05) years, and most of them were 1-9 years (175 cases, 40.51%) . Manufacturing industry (290 cases, 67.13%) and mining industry (78 cases, 18.06%) are the major industries; private economic enterprises (274 cases, 63.43%) and state-owned economic enterprises (74 cases, 17.13%) are the major economic types; small enterprises (295 cases, 68.29%) and medium-sized enterprises (75 cases, 17.36%) are the major enterprises. Conclusion We should strengthen the prevention and control of occupational diseases in key areas, people, industries, etc, and the employer, workers and government department should exercise their rights and obligations together to do a good job in the prevention and control relevant of occupational diseases.

Objective To explore the therapeutic effects of trimetazidine (TMZ) on diabetic patients with coronary heart diseases.Methods We conducted a comprehensive electronic search of PubMed, EMBASE, and Cochrane databases between the inception dates of databases and May 2019 (last search conducted on 30 May 2019) to identify randomized controlled trials. The evaluation method recommended by Cochrane Collaboration for bias risk assessment was employed for quality assessment. Random or fixed models were used to investigate pooled mean differences in left ventricular function, serum glucose metabolism, serum lipid profile, myocardial ischemia episodes and exercise tolerance with effect size indicated by the 95% confidence interval ().Results Additional TMZ treatment contributed to considerable improvement of left ventricular ejection fraction (=4.39, 95%: 3.83, 4.95, <0.00001), left ventricular end diastolic diameter (=-3.17, 95%: -4.90, -1.44, =0.0003) and left ventricular end systolic diameter (=-4.69, 95%: -8.66, -0.72, =0.02). TMZ administration also significantly decreased fasting blood glucose (=-0.43, 95%: -0.70, -0.17, =0.001), glycosylated hemoglobin level (=-0.59, 95%: -0.95, -0.24, =0.001), serum level of total cholesterol (=-20.36, 95%: -39.80, -0.92, =0.04), low-density lipoprotein cholesterol (=-20.12, 95%: -32.95, -7.30, =0.002) and incidence of myocardial ischemia episodes (=-0.84, 95%: -1.50, -0.18, =0.01). However, there were no significant differences in serum triglyceride level, high-density lipoprotein cholesterol, exercise tolerance between the TMZ group and the control group. Conclusion TMZ treatment in diabetic patients with coronary heart disease is effective to improve cardiac function, serum glucose and lipid metabolism and clinical symptoms.

Objective:To observe the performance of the traumatic brain injury (TBI) patients with normal results in Mini-Mental State Examination (MMSE) in the Montreal Cognitive Assessment (MoCA) and Rivermead Behavioral Memory Test-3 (RBMT-3). Methods:From March, 2015 to June, 2018，40 TBI patients with cognitive complaints but normally in MMSE and other 30 healthy persons (controls) were assessed with MoCA and RBMT-3. Results:There were 14 patients sounded normally in MoCA. The scores of MoCA, as well as both the raw score and scale score of RBMT-3, were less in the patients than in the controls (t > 2.822, P < 0.01), especially in the verbal and delayed recall subtests of MoCA (t > 2.790, P < 0.01), and delayed story recall, skill learning instant recall, delayed skills recall (both raw and scale scores) (t > 2.507, P < 0.05), and remember name delay recall and story instant recall (raw score only) (t > 2.018, P < 0.05) of RBMT-3. Conclusion:For TBI patients with cognitive complaints, it is necessary to assesse with both MMSE and MoCA. If there is any memory impairment, RBMT-3 is needed.

OBJECTIVE: To analyze the association of Nur77 with overall survival of gastric cancer patients and investigate the role of Nur77 in invasion and migration of gastric cancer cells. METHODS: Oncomine database was used to analyze the expression of Nur77 in gastric cancer and gastric mucosa tissues, and the distribution characteristics of Nur77 protein between gastric cancer and normal tissues were compared using Human Protein Atlas. GEPIA2 was used to analyze the relationship of Nur77 expression and the patients' survival. The expression of Nur77 in gastric cancer cell lines GES-1, AGS and MKN-45 were detected by Western blotting. The regulatory interactions between IL-6 and Nur77 were verified by transfecting the cells with specific Nur-77 siRNA and Nur-77-overexpressing plasmid. The changes in migration ability of the cells following Nur-77 knockdown were assessed with scratch assay. The effect of Nur-77 overexpression or IL-6 knockdown, or their combination, on migration and invasion of the gastric cancer cells were examined using Transwell assay. The effect of Nur77 expression level on NF-κB/IL-6 pathway activation was analyzed using Western blotting. RESULTS: Oncomine database showed that gastric cancer tissues expressed a significantly higher level of Nur77 mRNA than normal tissues (P < 0.05). Nur77 expression was detected mostly in the nucleus, and a high Nur77 expression was associated with a poor survival outcome of the patients (P < 0.05). In gastric cancer cells, the high expression of Nur77 participated in the regulation of IL-6. Nur77 silencing significantly lowered the migration ability of the cells (P < 0.05), and IL-6 silencing significantly attenuated the enhanced migration caused by Nur77 overexpression (P < 0.05). Nur77 participates in the activation of NF-κB/IL-6 signaling pathway by regulating the expression of p-p65, p65, p-Stat3 and Stat3. CONCLUSION A high Nur77 expression is strongly correlated with a poor prognosis of gastric cancer patients. Nur77 promotes the invasion and migration of gastric cancer cells possibly by regulating the NF-κB/IL-6 signaling pathway.
Cell Line, Tumor ; Cell Movement ; Gene Expression Regulation, Neoplastic ; Humans ; Interleukin-6/metabolism* ; NF-kappa B/metabolism* ; Neoplasm Invasiveness/genetics* ; Nuclear Receptor Subfamily 4, Group A, Member 1 ; RNA, Messenger/metabolism* ; RNA, Small Interfering/genetics* ; Stomach Neoplasms/genetics*

Objective: To reveal the effect of functional training & diet intervention on functional movement screen (FMS) and physical health of obese college male students. Methods: Totally 56 obese college male students were divided into experimental group and control group on average and the experimental group took functional training & diet intervention, 2 times per week, with a total of 15 weeks. The control group was only given routine physical exercise without diet intervention. Indexes including seven FMS indicators, seven body composition indicators and five physical fitness indicators were monitored before and after experiment. Results: The results showed that except for shoulder flexibility, other FMS indexes (vault, hurdle, straight lunge, trunk stability and trunk rotation stability) of experimental group significantly increased after functional training & diet intervention(t=-2.11, -2.27, -2.22, -2.08, -2.21, P<0.05). The total score of FMS increased significantly from 12.07 to 14.06(t=-4.22, P<0.05). The body composition index including fat content, body water, body fat ratio and BMI decreased to 23.97 kg, 38.83 kg, 26.34% and 27.79 kg/m2(t/χ2=-5.85, -8.30, 9.05, 7.23, P<0.05). Protein increased significantly to 19.00 kg(t=-5.75, P<0.05). The comprehensive score of body composition in the experimental group increased significantly from 64.00 to 66.71(t=-6.27, P<0.05). The scores of experimental group physical fitness indicators including 50 m, sit forward, standing long jump, pull up and 1 000 m increased to 8.24 s, 14.45 cm, 206.61 cm, 3.07 and 278.27 s(t=5.05, -3.96, -7.46, -3.62, 4.40, P<0.05). Conclusion The study indicates that 15 weeks of physical function training & dietary intervention could effectively improve the body shape, body composition and motor dysfunction of obese male college students, thus to reduce sports risk and improve physical health.

Objective To investigate the effects of lncRNA FENDRR on the proliferation, migration, invasion and apoptosis of LUSC H226 cells and its molecular mechanism. Methods The expression levels of FENDRR in normal lung epithelial cells BEAS, lung adenocarcinoma A549 and H1299 cells and LUSC H226 cells were detected by qRT-PCR. H226 cells were transfected with FENDRR-siRNA as the experimental group, or with FENDRR-siNC as a negative control group. Cell proliferation was detected by CCK-8 assay. Cell migration and invasion were detected by Transwell assay. Cell apoptosis was detected by flow cytometry. The protein expression levels of MEK, p-MEK, ERK and p-ERK were determined by Western blot. Results FENDRR levels in H226 cells were significantly lower than those in normal lung epithelial cells. Compared with the negative control group, the knockdown of FENDRR could significantly promote the proliferation, migration and invasion of H226 cells, inhibit the cell apoptosis, and increase the protein levels of p-MEK and p-ERK. The addition of ERK inhibitor U0126 rescued the effect of FENDRR knockdown on H226 cells. Conclusions The knockdown of lncRNA FENDRR can promote the proliferation, migration and inhibit the apoptosis of H226 cells through ERK/MAPK pathway.

This study aimed to evaluate the effect of sanguinarine on biomechanical properties of rat airway smooth muscle cells (rASMCs) including stiffness, traction force and cytoskeletal stress fiber organization. To do so, rASMCs cultured were treated with sanguinarine solution at different concentrations (0.005~5 μmol/L) for 12 h, 24 h, 36 h, and 48 h, respectively. Subsequently, the cells were tested for their viability, stiffness, traction force, migration and microfilament distribution by using methylthiazolyldiphenyl-tetrazolium bromide assay, optical magnetic twisting cytometry, Fourier transform traction microscopy, scratch wound healing method, and immunofluorescence microscopy, respectively. The results showed that at concentration below 0.5 μmol/L sanguinarine had no effect on cell viability, but caused dose and time dependent effect on cell biomechanics. Specifically, rASMCs treated with sanguinarine at 0.05 μmol/L and 0.5 μmol/L for 12 and 24 h exhibited significant reduction in stiffness, traction force and migration speed, together with disorganization of the cytoskeletal stress fibers. Considering the essential role of airway smooth muscle cells (ASMCs) biomechanics in the airway hyperresponsiveness (AHR) of asthma, these findings suggest that sanguinarine may ameliorate AHR via alteration of ASMCs biomechanical properties, thus providing a novel approach for asthma drug development.

OBJECTIVE: To review the clinical features of a male twin affected with glutaric academia type I (GA-I) and analyze the variations of glutaryl-CoA dehydrogenase (GCDH) gene. METHODS: Clinical data of the pair of twins and their parents were collected. Genomic DNA was extracted from peripheral blood samples, and variants of GCDH genes were detected by capture sequencing using a customized panel. Variants of the twins and their parents were verified by Sanger sequencing. RESULTS: The level of glutaric acyl carnitine (C5DC + C6OH) was 3.26 μmol/L in the male twin. The relative level of glutaric acid in urine was 547.51 by gas chromatography mass spectrometry analysis. Cerebral ultrasonography showed that the patient had subependymal hemorrhage, but no serious clinical manifestation was noted. After treating with special formula milk powder and L-carnitine, the boy showed good growth and development. Two heterozygous variants of the GCDH gene were detected in the patient, among which c.416C>G was suspected to be pathogenic, while c.109_110delCA was unreported. The variants were respectively inherited from his parents. The twin girl only carried the c.416C>G variant. CONCLUSION GA-I can be diagnosed by mass spectrometry, urine gas chromatographic mass spectrometry, imaging as well as genetic diagnosis. Early diagnosis and intervention is important.
Amino Acid Metabolism, Inborn Errors ; genetics ; Brain Diseases, Metabolic ; genetics ; Female ; Glutaryl-CoA Dehydrogenase ; deficiency ; genetics ; Humans ; Male ; Mutation ; Phenotype

Objective: To explore the characteristics of event-related potential(ERP) in the development of alert attention of children aged 6-11 years old. Methods: A total of 144 children aged 6-11 years old were enrolled in a continuous performance test (CPT, Go/Nogo stimulus task). The latency and amplitude of frontal region cue/uncue-P3 and CNV were analyzed and compared in all age groups. Results: (1)Behavioral comparison among different age groups: correct number(35(31, 38), 37(34, 38.25), 37(34.75, 39), 38(35, 39.25), 39(37.25, 40), 39(39, 40)), correct reaction time((566.88±86.63)ms, (530.10±83.40)ms, (509.17±88.99)ms, (488.31±76.23)ms, (463.45±75.78)ms, (417.24±75.78)ms), the differences were statistically significant(P<0.01). (2)Fz lead ERP characteristics: ①Comparison of cue-CNV and uncue-CNV: 6-year-old group((-8.54±4.56)μV vs (-10.13±6.36)μV), 7-year-old group((-9.20±5.34)μV vs (-11.53±4.29)μV), 8-year-old group((-8.37±4.78)μV vs (-11.35±6.38)μV)in amplitude and 9-year-old group((689.25±268.22)ms vs (826.13±174.62)ms)in latency were significantly different(P<0.05). ②Comparison of cue-P3 and uncue-P3: 6-year-old group((4.29±4.11)μV vs (7.45±3.83)μV), 7-year-old group((4.47±4.71)μV vs (5.82±4.40)μV), 8-year-old group((3.76±3.39)μV vs (6.24±4.91)μV), 9-year-old group((4.27±3.57)μV vs (5.93±3.08)μV), 11-year-old group((4.24±4.66)μV vs (6.13±4.42)μV)in amplitude were significantly different(P<0.05). ③Longitudinal comparison among age groups: the latency of cue-CNV in 9-year-old group and 11-year-old group were shorter, and the difference was significant(P<0.05). The amplitudes of cue-CNV and cue-P3 in 10-year-old group were shorter than those in other age groups(P<0.05). Conclusion With the increase of age, the reaction time of school-age children shortened and the correct rate improved.The age of 9-10 may be the high-speed period of the development for brain network on vigilant attention and psychological prediction which may be related to the maturation and development of frontal cortex.

Objective: To investigate the potential independent risk factors of body mass rebound following laparoscopic sleeve gastrectomy (LSG) and construct a nomogram prediction model based on these factors. Methods: In this retrospective observational study, patients with obesity who had undergone LSG at the Department of Gastrointestinal Surgery of the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University between January 2015 and July 2017 were retrospectively enrolled. These patients were divided according to their status of postoperative body mass rebound. The inclusion criteria were patients aged between 16 and 65 years who had undergone LSG bariatric surgery with surgical indications according to the 2014 Chinese Guidelines for the Surgical Management of Obesity and Type 2 Diabetes Mellitus. The exclusion criteria were patients who had undergone other bariatric surgeries, who were taking weight-loss drugs or drugs that affected their body weight, who had severe gastroesophageal reflux and hiatal hernia, who were pregnant, who had incomplete clinical data, and who were lost to follow-up or were followed up for <3 years. In total, 241 patients with obesity (69 males and 172 females) who had undergone LSG surgery were enrolled. The mean age and body mass index (BMI) were (29.9±5.8) years and (40.8±4.8) kg/m², respectively. The patients were followed up till July 2022, with a focus on their body weight. Postoperative body mass rebound was defined as a percentage increase of ≥10% from the nadir body mass, which was the lowest body mass during the 3-year follow-up period. The body weight rebound following LSG and its influencing factors were observed, based on which a nomogram model was constructed and evaluated. The relationships between the patients' basic data, clinical indicators, preoperative hematological indicators, postoperative indicators, and body weight rebound following LSG were analyzed via univariate analysis. Independent risk factors were further screened by multivariate logistic regression analysis. Factors with a statistically significant difference were included into the nomogram prediction model. Moreover, the model was internally (modeling set) and externally (validation set, 80 baseline data-matched patients with obesity from our center) validated using receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) via R software. ROC curve analysis was used to analyze the predictive and cutoff values of the measurement data for body mass rebound. Results: Overall, 90 patients (37.3%) exhibited postoperative body weight rebound, with the lowest BMI of (29.5±2.6) kg/m² and time to reach the lowest BMI of (15.4±2.3) months; 151 patients (62.7%) did not exhibit body weight rebound, with the lowest BMI of (29.8±2.3) kg/m² and time to reach the lowest BMI of (14.7±2.1) months. The results of univariate analysis showed that BMI, depression, anxiety, C-reactive protein (CRP) levels, systemic immune inflammatory index (SII), prognostic nutritional index (PNI), and albumin/fibrinogen ratio (AFR) were associated with body weight rebound following LSG with statistically significant differences (all P<0.05). The results of multivariate regression analyses suggested that depression [odds ration (OR) = 1.31, 95% confidence interval (CI): 1.08-1.62, P=0.010], preoperative CRP levels of ≥8 mg/L (OR = 1.34, 95% CI: 1.09-1.69, P=0.007), SII (OR = 0.58, 95% CI: 0.41-0.86, P=0.013), PNI (OR = 2.06, 95% CI: 1.03-4.21, P=0.007), and AFR (OR: 0.49, 95% CI: 0.33-0.69, P=0.011) were five independent risk factors for body mass rebound. A nomogram prediction model was constructed based on the multivariate analysis results. The scores of PNI, SII, AFR, CRP, and depression were 92.5, 100, 72.5, 25, and 27.5, respectively. The total score was calculated by adding the individual scores of each risk factor, which was used to calculate the probability of body mass rebound following LSG. The evaluation results of the nomogram model showed a C-index of 0.713 and 0.762, sensitivity of 0.656 and 0.594, and specificity of 0.715 and 0.909 in the modeling and validation sets, respectively. The calibration curve analysis and DCA indicated that the nomogram model has a good predictive value for body mass rebound after LSG. Conclusion: Preoperative depression, CRP of ≥8 mg/L, SII, PNI, and AFR were independent risk factors for body mass rebound following LSG. Hence, the nomogram prediction model based on these factors can effectively predict body mass rebound in patients undergoing LSG.
Adolescent ; Adult ; Aged ; Anti-Obesity Agents ; C-Reactive Protein ; Diabetes Mellitus, Type 2 ; Factor Analysis, Statistical ; Female ; Fibrinogen ; Gastrectomy/methods* ; Humans ; Laparoscopy/methods* ; Male ; Middle Aged ; Nomograms ; Obesity ; Retrospective Studies ; Risk Factors ; Weight Loss ; Young Adult