Object To study the effect of pH on callus tissue growth and paclitaxel content of Taxus cuspidata Sieb. et Zucc. and Taxus chinensis var. mairei (Lemee et Le ′vl.) Cheng et L. K. Fu Methods The callus tissues were cultured on B5 medium with defferent pH values, and the growth rate, PAL activity and paclitaxel content were determined. Results Different callus tissues of taxus need different optimum pH values. The pH values, which could promote callus growth significantly, were all inhabitory to PAL activity and accumulation of paxlitaxel. Conclusion pH had great influences on the growth of the callus and the level of secondary metabolism, thereby the synthesis and accumulation of paclitaxel were affected.

Objective: To analyze the efficacy and safety of intervertebral endoscopy in the treatment of double segmental protrusion of lumbar intervertebral disc. Methods: From February 2016 to May 2017, 126 patients with double segment lumbar disc herniation diagnosed in Rizhao Central Hospital were divided into the observation group and the control group according to the different treatment methods, with 63 cases in each group.The observation group was treated with intervertebral foramen technique, and the control group was treated with conventional fenestration.The treatment efficacy of the two groups was compared.The differences of VAS score and ODI between the two groups before and after operation were compared. Results: Compared with the control group, the observation group had shorter hospitalization time[(7.1±3.1)d vs.(11.5±4.2)d], smaller incision length[(1.2±0.3)cm vs.(4.3±1.6)cm], less blood loss[(22.3±8.2)mL vs.(36.3±9.2)mL], there were statistically significant differences between the two groups (t=9.365, 3.965, 5.566, all P<0.05). From preoperation to 6 months after operation, the VAS scores of the two groups showed a significant downward trend.There were statistically significant difference in the VAS scores between the two groups at different time points, between groups and at different time points (F=5.688, 4.589, 3.998, all P<0.05). From preoperation to 6 months after operation, the ODI of the two groups decreased first and then increased, and there was statistically significant difference between the two groups of ODI in different time points (F=4.254, P<0.05). The good rate of curative effect in the observation group was 87.3%, which in the control group was 84.1%, the difference was not statistically significant (χ²=0.259, P>0.05). There was no statistically significant difference in the incidence rate of adverse reactions between the two groups (6.4% vs.9.5%) (χ²=0.434, P>0.05). Conclusion The treatment of double segment lumbar intervertebral disc herniation with intervertebral foraminoscopy is reliable, with small tissue trauma and mild pain.