1.An investigation on the relationship between sulfonylurea receptor 1 gene intron 24-3t/c polymorphism and blood lipids in Chinese population
Yan FENG ; Qiongfang LI ; Changzhong CHEN
Chinese Journal of Diabetes 2000;0(05):-
Obeject To study the association of sulfonylurea receptor (SUR) 1 gene and blood lipids level.Method We investigated the SUR1 gene intron 24 3t/c polymorphism by polymerase chain reaction (PCR) and appropriate restriction enzyme (PCR RFLP) in 132 couples of type 2 diabetic case control and 282 type 2 diabetic pedigrees. The statistical methods were t test, multiple line regression and family based association test (FBAT).Result In the control group, the level of total cholesterol (TC), low density lipoprotein (LDL) and Apo B were higher in the cc genotype than that in the tt and the tc genotype. FBAT showed that sulfonylurea receptor 1 gene intron 24 3t/c polymorphism was significantly associated with TC, ApoB and BMI.Conclusion Sulfonylurea receptor 1 gene intron 24 3t/c polymorphism is associated with blood lipid level in north Chinese population.
2.Experimental study of Ti-75 alloy as a substrate of composite implant
Kejun HUB ; isheng CHEN ; Changzhong TAO ; Xiaozhong Jiang ; Zhixiong YANG
Journal of Practical Stomatology 1995;0(04):-
Objective:To evaluate the biocompatibility of Ti-75 alloy(TA) as a substrate of composite implant (CI) and the role of the shear strength of TA-coating in the CI-bone interface. Methods:A transcortical implant model was used. A total of 32 implants of CI and TA were implanted into bilateral femur of 4 adult mongrel dogs. the animals were killed 3 and 6 months after surgery. The implant-bone interfaces were observed by morphological examination and the interface shear strength was measured by push-out test. After push-out test, the failure interfaces were observed by SEM. Results:Osseo-integration was confirmed in the both interfaces of CI and TA with bone. The interface strength(MPa) of CI in 3 and 6 month was 7. 84? 0.99 and 12. 76? 1. 45, that of TA 3. 71? 1. 10 and 6. 33? 0. 89 respectively (P
3.Association of GYS1 and beta(3)-AR gene with postprandial hyperglycemia and serum uric acid in type 2 diabetes mellitus.
Guoying WANG ; Qiongfang LI ; Tianhua NIU ; Changzhong CHEN ; Xiping XU
Chinese Medical Journal 2002;115(9):1308-1311
OBJECTIVETo determine the relationships of Met416Val and XbaI polymorphism of muscle glycogen synthase (GYS1) gene and Trg64Arg variant of the beta(3)-adrenergic-receptor (beta(3)-AR) gene with type 2 diabetes mellitus (DM) and its intermediate phenotypes in the Chinese population.
METHODSPolymerase chain reaction-oligonucleotide ligation assay and restriction fragment length polymorphism assay were used to evaluate the GYS1 and beta(3)-AR gene polymorphisms in 102 pairs of case-control Chinese spouses.
RESULTSSubjects with Met416Val variant had a significantly higher 2-hour post-glucose level than subjects without this variant had in diabetic group (P = 0.032). The Met416Val polymorphism of GYS1 gene was not significantly associated with the risk of type 2 DM (adjusted OR = 1.67; 95% CI: 0.73 - 3.81, P = 0.223). Subjects with Trp64Arg variant had a significantly higher serum uric acid level than subjects without this variant had in diabetic group (P = 0.034). The combination of BMI and Arg64 allele carrier of the beta(3)-AR gene increased the diabetic risk over four-fold (adjusted OR = 4.00; 95% CI: 1.53 - 10.45, P = 0.005).
CONCLUSIONSIn the Chinese population, Met416Val polymorphism is identified in a subgroup of diabetic subjects with high 2-hour post-glucose. It will explain why some diabetic patients appear to be genetically predisposed to developing high postpradial glucose level. The presence of the Arg64 allele in the beta(3)-AR gene may predispose patients to higher serum uric acid level.
Adult ; Aged ; Alleles ; Body Mass Index ; Diabetes Mellitus, Type 2 ; blood ; genetics ; Glycogen Synthase ; genetics ; Humans ; Hyperglycemia ; genetics ; Middle Aged ; Polymorphism, Genetic ; Postprandial Period ; physiology ; Receptors, Adrenergic, beta-3 ; genetics ; Uric Acid ; blood
4.Progress in study of self-assembling peptides.
Yuanwei CHEN ; Changzhong ZHANG ; Tianquan LI ; Changxiu WAN
Journal of Biomedical Engineering 2006;23(1):209-211
Self-assembly of peptides is ubiquitous in the body of creatures. The molecules of peptides combine with each other to form proteins with different functions through self-assembly. The formation of a specific conformation of one type of protein is owing to the self-assembly of its compositive amino acids. So, researchers can design self-assembly of peptides at the molecular level and can control its formation and configuration. It has the potential for application in the preparation of new medicines and biomaterials. In recent years, self-assembling peptides have been increasingly high-lighted and used to simulate the function of natural biomolecules, to synthesize peptide-medicine, and to serve as the carriers of medicine.
Biocompatible Materials
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chemical synthesis
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Molecular Conformation
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Nanotechnology
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methods
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Peptides
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chemistry
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Protein Engineering
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methods
5.IL-17 Imbalance Promotes thePyroptosis in Immune-Mediated L
Wenfang XU ; Yanan WANG ; Changzhong JIN ; Weiyang ZHANG ; Jiangnan CHEN ; Xuefang CHEN ; Junli GAO ; Junshun GAO ; Hong WANG
Immune Network 2023;23(6):e46-
Autoimmune hepatitis (AIH) affects all age group and occurs mainly in women. Pyroptosis is a novel programmed cell death featured with cell bursting and release of proinflammatory cytokines. A deeper understanding of AIH pathogenesis will contribute to novel therapy for AIH patients. Here, we aimed to investigate the role of IL-17 in immune-mediated liver injury.The levels of cytokines were measured by ELISA, and mRNA levels of STAT3 and IFN gammainducible protein 16 (IFI16) were detected by PCR. Expressions of STAT3, IFI16, gasdermin D and cleaved caspase-1 were measured by western-blotting. Immunohistochemical staining and transmission electron microscopy were applied to evaluate liver histopathological changes of the treated mice. Our results showed that the levels of IFI16 was increased in hepatocytes treated with IL-17 protein, and further elevated after STAT3-overexpressed (STAT3-OE) lentivirus treatment. The levels of IFI16 were reduced in hepatocytes treated with IL-17 neutralizing Ab (nAb), but were significantly increased after STAT3-OE treatment. Pyroptosis was observed in hepatocytes treated with IL-17 protein, and further cell damage was observed after STAT3-OE lentivirus treatment. Liver damage was alleviated in mice treated with IL-17 nAb, however sever damage was experienced after STAT3-OE lentivirus treatment. A binding interaction between IFI16 and STAT3 was detected in IL-17 treated hepatocytes. Glutathione transaminase activity was enhanced in concanavalin A-induced AIH mice compared to the control group (p<0.01). IL-17 plays an important role in activating STAT3 and up-regulating IFI16, which may promote the pyroptosis in AIH-related liver injury through STAT3-IFI16 axis.
6.Way of 'analytical thinking' on data from epidemiological studies.
Chinese Journal of Epidemiology 2014;35(6):745-748
Analysis on data from epidemiological studies is the sequencing process of applying statistical methods to collected data from different angles, interpreting intermediate results, drawing statistical conclusions and forming scientific findings based on existing knowledge. This is also called the 'process of converting data to evidence'. Final results from the analysis are expressed through scientific papers. Process of an accurate, clear and comprehensive data analysis is critical to form a convincing conclusion on a paper. This article discusses how to form the analytical thoughts for conducting a thorough data analysis in order to draw a convincing evidence from epidemiological data.
Data Interpretation, Statistical
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Epidemiologic Studies
7.Way of‘analytical thinking’ on data from epidemiological studies
Chinese Journal of Epidemiology 2014;(6):745-748
Analysis on data from epidemiological studies is the sequencing process of applying statistical methods to collected data from different angles,interpreting intermediate results, drawing statistical conclusions and forming scientific findings based on existing knowledge. This is also called the‘process of converting data to evidence’. Final results from the analysis are expressed through scientific papers. Process of an accurate,clear and comprehensive data analysis is critical to form a convincing conclusion on a paper. This article discusses how to form the analytical thoughts for conducting a thorough data analysis in order to draw a convincing evidence from epidemiological data.
8.Advances of the mechanism study on Chinese herb monomers in improving premature ovarian failure
Rugen YAN ; Yuqi WANG ; Jing HE ; Yin CHEN ; Pingping CAI ; Changzhong LI
China Pharmacy 2022;33(21):2685-2688
Premature ovarian failure (POF) is a common gynecological endocrine disease ,which can cause follicular development stagnation or premature failure in the ovary ,leading to ovarian function loss ,and seriously affecting women ’s physical and mental health . Traditional Chinese medicine shows less toxicity and side effects but significant effect in the treatment of POF . Chinese herb monomers are the active ingredients of traditional Chinese medicine that exert their effects ,and their chemical structure are determined . From the perspective of Chinese herb monomers ,the author reviewed the mechanism of their effect on improving POF . The results showed that icariin ,quercetin,resveratrol and ginsenoside Rg 1 could improve the ovarian function of POF model animals by activating the phosphatidylinositol 3-kinase/protein kinase B signaling pathway ,inhibiting oxidative stress and apoptosis of granulosa cells ,promoting ovarian autophagy ,and regulating the level of sex hormones ;puerarin and resveratrol can improve the ovarian function of POF model by interfering with Wnt/ β-catenin signaling pathway ,reducing oxidative stress response ,and inhibiting follicular atresia . Although the Chinese herb monomers have certain effect in improving POF,most of them remain in the experimental stage ,and the clinical efficacy in improving POF cannot be objectively evaluated . In the future ,the development of new dosage forms of Chinese herb monomers should be strengthened to lay a foundation for the development of new drugs for treating POF .