1.Effect of allergen at different concentrations on the production of IL-5 by T cells of asthmatic mice
Journal of Third Military Medical University 2003;0(16):-
Objective To investigate the effects of allergen at different concentrations on the production of IL 5 by lymphocytes of mice with asthma and its mechanism. Methods The levels of IL 5 produced by T cells treated with allergen of different concentrations and monocytes as antigen presenting cells in vitro were measured by ELISA and the apoptosis rate of T lymphocytes was determined by TUNEL. Results ① The administration of high dose of allergen inhibited the expression of IL 5( P
3.Effects of transfecting immature dendritic cells with indoleamine 2,3-dioxygenase gene on the spleen CD4~+ T cells in ovalbumin-allergized mouse model
Xiaojing AN ; Guisheng QIAN ; Changzheng WANG
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
Objective To observe the effects of mouse indoleamine 2,3-dioxygenase (IDO) gene on CD4+ T cells by transfecting an eukaryotic expression vector containing mouse IDO gene fused with enhanced green fluorescent protein (pEGFP-N1). Methods The immature dendritic cells (imDCs) derived from mouse bone marrow were cultured in vitro and morphologically identified by transmission electron microscope, scanning electron microscope and flow cytometry. The immature dendritic cells were transfected by DOTAP liposome, and the expression of green fluorescence protein was confirmed with inverted fluorescence microscope. The spleen-derived CD4+ T lymphocytes from mice were isolated and purified in vitro, and then carried to mixed lymphocyte reaction (MLR) with 3H-TdR incorporation. The effect of IDO gene on the proliferation of spleen-derived CD4+ T lymphocytes of the allergized mouse challenged by ovalbumin was assayed by 3H-TdR MLR incorporation, and the apoptosis of spleen-derived CD4+ T lymphocytes was assayed by TUNEL. Results The proliferation of spleen-derived CD4+ T lymphocytes of the allergized mouse challenged by ovalbumin were inhibited by the imDCs transfected with IDO gene, the number of CD4+ T lymphocytes treated with the imDCs transfected pEGFP-N1-IDO eukaryotic expression vector decreased significantly compared with that in control group (P
4.Comparison of the bronchial obstruction reversibility in patients with chronic obstructive pulmonary disease and with asthma
Zhiqiang ZHAO ; Jinping WANG ; Changzheng WANG ;
Journal of Third Military Medical University 2003;0(07):-
Objective To explore the bronchial obstruction reversibility in patients with chronic obstructive pulmonary disease, and to conduct a comparison with that in patients with asthma. Methods The pulmonary ventilation function of patients with chronic obstructive pulmonary disease(COPD) and with asthma before and after inhaling salbutamol inhaler was separately determined with 6200 Autobox DL, and all corresponding parameters of in two groups were compared. Results No difference of the corresponding parameters of baseline ventilation function in two groups was found. The pulmonary function of patients in two groups was obviously improved after inhalation of drug( P
5.Side effects of inhalation of hyperosmotic saline on sputum induction in asthmatic patients
Yan WANG ; Changzheng WANG ; Kexiong LIN ;
Journal of Third Military Medical University 2003;0(07):-
0 05). Excessive bronchoconstriction occurred in one patient (1/41, 2.44%). Nausea and vomiting, pharyngeal dryness and pharyngalgia, dizziness and headache, discomfort of chest, and so on were found in other 43 9% patients, but the side effects were mild and endurable. Conclusion Sputum induction after inhalation of short acting ? 2 agonist is safe for asthmatic patients in the remission stage.
6.The determination and the clinical significance of maximal forced inspiratory flow volume curve
Jucai JIA ; Jinping WANG ; Changzheng WANG
Journal of Third Military Medical University 1983;0(03):-
The procedure to delineate the maximal forced inspiratory flow volume curve(MIFVC)was described and the curve was determined in 56 normal subjects to serve as the control and 62 patients with various chronic obstructive pulmonary diseases(COPD).It was found that MIFVC was significantly lower in patients with various COPD than in the control(P
7.Expressions of the common β subunit and specific α subunit of IL-5, IL-3, and GM-CSF receptor mRNA in eosinophils in BALF from guinea pigs with asthma
Zhikui LI ; Changzheng WANG ; Guisheng QIAN
Journal of Third Military Medical University 2001;23(2):146-148
Objective To investigate the relationship of the expressions of common β (βcR) and specific α chains of IL-5, IL-3 and GM-CSF receptors with the eosinophils(Eos) apoptosis for the roles of these receptors in asthma. Methods All of 12 guinea pigs were equally randomized into normal and ovalbumin sensitized asthmatic group. The apoptosis of hypodense and normodense Eos in bronchoalveolar lavage fluid (BALF) was detected by TUNEL method. The expression of common β and specific α chains of the 3 receptor mRNA were measured with RT-PCR and in situ hybridization. Results More Eos were observed in asthmatic group than in normal group, especially those in hypodense (P<0.01). The apoptotic rate of Eos in BALF were significantly lower in asthmatic group than in normal animals (P<0.01), but no difference was found between hypodense and normodense Eos. Compared with normal group, the expressions of the 3 receptor mRNA α chains were decreased in asthmatic group than in normal group (P<0.01), but βcR expression was increased significantly. More IL-3 Rα and GM-CSFRα mRNA were expressed in hypodense Eos than in normodense Eos. The contents of IL-5Rα and IL-3Rα mRNA were lower in asthmatic group than in normal group. Conclusion The apoptosis of Eos is inhibited in BALF from guinea pigs with asthma. The expressions of IL-5, IL-3 and GM-CSF receptors in Eos are regulated through 2 mechanisms: the reduced expressions of their specific α chains which attenuating the negative regulation on Eos activation and inhibited apoptosis and the increased expressions of their βcR which enhancing the positive regulation on the 2 aspects. These data suggest that IL-5, IL-3 and GM-CSF receptors might be involved in the pathogenesis of asthma through regulating the apoptosis of Eos.
8.Effects of Aminophylline on expression of Fas mRNA and apoptosis in eosinophils
Zhikui LI ; Changzheng WANG ; Guisheng QIAN ;
Chinese Pharmacological Bulletin 1986;0(05):-
AIM To investigate the effects of aminophylline (AM) on expression of Fas mRNA and apoptosis in eosinophils(Eos) of bronchalveolar lavage fluid(BALF) in asthmatic guinea pig, and the mechanism of AM promoting Eos apoptosis. METHODS After guinea pigs were stimulated by ovalbumin(OVA) 48 h, hypodense Eos(HEos) and normodense Eos(NEos) were purified from BALF by gradients of percoll. Purified Eos were incubated with different concentration of AM (10 -6 ~10 -3 mol?L -1 ) in RPMI 1640 medium 24 h, the mRNA of Fas in Eos was measured by hybridization and apoptosis was detected by TdT mediated dUTP nick end labeling. RESULT Eos cultured under the condition of AM administration in vitro, apoptotic Eos increased and the expression of Fas mRNA increased in a dose dependent manner. The expression of Fas mRNA was correlated with Eos apoptosis. CONCLUSION AM promote Eos apoptosis and expression of Fas in dose dependent manner in vitro. Fas might be one of mechanism that AM promoting apoptosis of Eos.
9.Expression of the Bcl-2 and Bax mRNA in eosihophils of BALF in guinea pig with asthma
Zhikui LI ; Changzheng WANG ; Guisheng QIAN
Chinese Journal of Immunology 2000;0(08):-
Objective:To investigate the relation between expression of Bcl-2,Bax and eosinophils(Eos) apoptosis,explore effect of Bcl-2 and Bax on asthma.Methods:Guinea pigs were divided into normal and asthmatic group randomly. Ovalbumin was used to prepare model of asthma in guinea pig. Apoptosis of Eos in BALF was detected.mRNA expression of Bcl-2 and Bax in Eos were measured by hybridization and RT-PCR.Results:Comparing with normal group,Eos in BALF from asthmatic group were significantly higher; the percentage of apoptotic Eos from asthmatic group were significantly lower( P
10.The protective effect of metformin on the myocardium of diabetic rats
Yuanzheng WANG ; Xiaoming ZHAO ; Changzheng MU
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
0.05). The pathological change in myocardial ultrastructure was markedly milder in DM rats. Conclusion Metformin may exert a certain protective effect on cardiomyopathy of diabetes in rats.