1.Analysis of test results using residual reagents in electrochemiluminescence immunoassay analyzer
Wenli XU ; Changzheng LIAO ; Yanhua LIN
International Journal of Laboratory Medicine 2014;(18):2529-2530
Objective To evaluate the detection efficiency of residual reagent used in electrochemiluminescence immunoassay an-alyzer .Methods Alpha fetoprotein (AFP) ,carcino-embryonic antigen(CEA) and total prostate specific antigen(TPSA) were de-tected by using remaining reagents .The precision and recovery rate of remaining reagents were analyzed .Referring to the document of NCCLS EP9-A2 ,8 clinic samples were detected everyday by residual reagent and new reagent respectively .The test results of to-tal 40 samples were recorded within 5 days .The test results derived from 2 kinds of reagents were analyzed comparatively and their bias was evaluated by using new reagent as a control method and residual reagent as experimental methods .Results The within-run and between-run coefficients of variation(CV) of the 3 items measured by residual reagents in low and high levels of quality control products met the related standard .The recovery rate was variable from 90% to 110% .The test rewults of the 2 kinds of reagents were positively correlated(r2 >0 .95) .Their anticipated biases were within allowed biases on the medical decision level of CEA ,AFP and T PSA .Conclusion Residual reagent of electrochemiluminescence immunoassay analyzer can meet the clinical practice needs , which also can ensure the quality of measurement and the reduction of the cost .
2.Side effects of inhalation of hyperosmotic saline on sputum induction in asthmatic patients
Yan WANG ; Changzheng WANG ; Kexiong LIN ;
Journal of Third Military Medical University 2003;0(07):-
0 05). Excessive bronchoconstriction occurred in one patient (1/41, 2.44%). Nausea and vomiting, pharyngeal dryness and pharyngalgia, dizziness and headache, discomfort of chest, and so on were found in other 43 9% patients, but the side effects were mild and endurable. Conclusion Sputum induction after inhalation of short acting ? 2 agonist is safe for asthmatic patients in the remission stage.
3.Chitosan/PVA nerve conduits repair sciatic nerve defect in rats
Yong LIU ; Chunlin HOU ; Haodong LIN ; Zhen XU ; Changzheng WEI
Chinese Journal of Microsurgery 2011;34(4):297-300
ObjectiveTo investigate the effects of chitosan/PVA nerve conduits which used for repairing sciatics nerve defect in rats.MethodsTwenty-seven rats were divided into 3 groups randomly,with 9 rats in each group. Firstly, the 15mm defects in the left sciatic nerves were made in the rats and were respectively repaired with chitosan/PVA conduits graft (group A), the silicon conduits graft (group B),and autografts (group C). At 12 weeks after the operations, the left sciatic nerves were taken out, and the comparative evaluation was made on the repairing effects by wet weight of gastrocnemius and soleus muscles, histological examination,computerized imaging analysis and True Blue retrograde tracing. ResultsThe wet weight of gastrocnemius and soleus muscles showed no significant difference between the chitosan/PVA graft and autograft groups (P > 0.05). The wet weight of gastrocnemius and soleus muscles in significant difference between the chitosan/PVA graft and the silicon group at 12 weeks after the operation(P < 0.05). The nerve fiber density showed no statistically significant differences between the chitosan/PVA and autograft groups(P> 0.05).The regenerative nerve fiber in group B had normal morphological and structural characters under transmission electron microscope.True Blue-labeled neuron cell bodies were found within both anterior horn of gray matter in the spinal cord and dorsal root ganglions (DRGs) ipsilateral to the operated side of the tested rats on illumination with ultra-violet light 1 week after the injection of True Blue.Conclusion Chitosan/PVA nerve conduit can effectively promote the nerve regeneration and myelinization of rat sciatic nerve, which is expected to substitute for autograft to repair nerve defects succesfully.
4.The effects of dendritic cells overexpressing Serrate1 on the differentiation of CD4+ CD25+ T cells in asthmatic mice
Kun SUN ; Kexiong LIN ; Changzheng WANG ; Kui WU
Chinese Journal of Internal Medicine 2009;48(9):756-759
ction of immune tolerance of T cells to allergens.
5.Effects of modified fat clearance technique in the diagnosis of metastatic lymph nodes in the mesorectum of patients with rectal cancer
Yunfeng YAO ; Lin WANG ; Yiqiang LIU ; Changzheng DU ; Jin GU
Chinese Journal of Digestive Surgery 2009;8(2):140-143
Objective To investigate the number, distribution and metastatic rule of lymph nodes in the mesorectum of rectal cancer specimen after processing the recta] cancer specimen with modified fat clearance technique. Methods Sixty patients with mid-low rectal cancer who had been admitted to Peking University School of Ontology from 2003 to 2008 were assigned to test group. All the 60 patients who denied neo-adjuvant therapy were treated with total mesorectal excision, and the rectums resected were processed with the modified fat clearance technique. Rectums from another 50 patients with mid-low rectal cancer in control group were fixed in formalin solution for 24 hours. The mesorectum was divided into anterior, posterior, left and right quadrants, and each quadrant was further divided into upper, middle and lower parts. The numbers of lymph nodes harvested and metastatic rate of lymph nodes between the 2 groups were analyzed by t test and chi-square test. Results (1) The numbers of lymph nodes harvested in the test and control groups were 1436 and 525, with statistical difference between the 2 groups (t =- 12. 153, P <0.05). The number of small lymph nodes(diameter≤5 mm) harvested in test group was 985. (2) The numbers of lymph nodes harvested in the anterior, posterior and bilateral mesorectum were 125,696 and 615, respectively. The numbers of lymph nodes harvested in the upper, middle and lower part of the mesorectum were 395,534 and 507, respectively. The metastatic rate of lymph nodes in the upper part of the mesorectum was 18.5% (37/200), which was significantly lower than in the middle [43.5% (87/200)] and lower [38.0% (76/200)] part of the mesoreetum (X2= 9. 414, 6.406, P < 0.05). Two hundred metastatic lymph nodes in 33 patients were harvested, 48.0% (96/200) of which with a diameter of ≤ 5 mm. Twenty percent patients had their TNM stage changed after the retrieval of metastatic small lymph nodes. Conclusions Modified fat clearance technique significandy improves the retrieval of lymph nodes, and more small lymph nodes retrieved can increase the accuracy of staging. Rectal cancer cells have strong tendency to disseminate to the distal mesorectum. Adequate excision of the distal mesorectum is key in controlling the local recurrence.
6.Cost-effectiveness Analysis of Six Therapeutic Schemes for Upper Urinary Tract Infections
Yanmei FENG ; Aijun ZHANG ; Jinqiu WANG ; Changzheng LIN
China Pharmacy 2001;12(6):343-344
OBJECTIVE: To evaluate the economic effectiveness of different pharmacotherapeutic schemes for the same disease.METHODS: Using pharmacoeconomical cost-effectiveness analysis, six schemes for upper urinary tract infections were compared.RESULTS: The total effective rate and cost were both the lowest in group B(70.00% ).The cost-effectiveness ratios of A, B, C, D, E, F were 28.60、 19.13、 43.27、 29.99、 43.02、 29.14, respectively.They became 23.65、 18.01、 34.00、 24.87、 33.91、 24.20 by sensitivity.CONCLUSION: The A and F were both the most inexpensive and effective schemes.
7. Problems and countermeasures in liver stem cell research and application
Academic Journal of Second Military Medical University 2017;38(9):1092-1097
Stem cell has been one of the focuses in current biomedical research. It has been well documented that there are various liver stem cells (LSCs) with different markers in liver, and they play important roles in liver regeneration. Moreover, some progresses have been made in autologous and allogeneic stem cells transplantation for the treatment of end-stage liver diseases. However, the origin and markers of LSCs have not been fully clarified, and the mechanism involved in LSCs proliferation and differentiation remains to be deeply studied. With the improvements of lineage tracing technique and stem cell isolation and culture system, it is believed that the origin of LSCs and their role in liver regeneration will be further clarified, and LSCs treatment for liver diseases will soon become a reality.
8. Cranioplasty following decompressive craniectomy; an analysis of complications
Academic Journal of Second Military Medical University 2012;33(10):1148-1150
Objective To discuss the incidence, influencing factors and management of various complications following cranioplasty. Methods The clinical data of 302 patients who received cranioplasty in our hospital from Jan. 2005 to Jan. 2011 were retrospectively analyzed. Results Fifty-two (17. 22%) patients were found to have complications after operation, with 10 of them having more than two complications. The complication rates in patients with preoperative Glasgow Coma Scale (GCS) scores of GCS 3-5, GCS 6-8, GCS 9-12, and GCS 13-15 were 44. 44%, 29. 63%, 13. 98%, and 9. 24%, respectively (P< 0.001). Conclusion The complication rate following cranioplasty increases with the severity of brain injury; measures should be taken for early prevention, discovery, and treatment so as to obtain a better prognosis.
9.Protection of Pingkui Powder from acute gastric ulcer in rats
Changzheng LIN ; Yongxiao CAO ; Changshun LI ; Jing LIU ;
Chinese Traditional Patent Medicine 1992;0(02):-
AIM: To study the action of Pingkui Powder (Herba Gynostemmatis Pentaphylli, Fructus Hippophae, etc.) on the experimental acute gastric ulcer and its influence on gastric mucosa injury in rats. METHODS: The experimental models were established by means of histamine and indomethacin, area of ulcer was measured and quantity of gastric mucosa secretion of rats was analyzed. RESULTS: Pingkui Powder could reduce areas of ulcers, promote the gastric mucosa secretion. CONCLUSION: Pingkui Powder can improve the healing of acute ulcer and promote gastric mucosa secretion.
10.Comparative study on immunoregulation of sulfated propylene glycol alginate and its fractions
Changzheng LIN ; Huashi GUAN ; Guangli YU ; Haihua LI ; Caixia GU
Chinese Journal of Marine Drugs 2001;0(05):-
Objective To investigate the influence of sulfated propylene glycol alginate(PSS) and its fractions on the immunoregulation.Methods The immunoregulation activity of PSS and its fractions were investigated by using immunocyte cultivation technique in vitro.The structure activity relationship was analysed on the basis of the structure studies of PSS' fractions.Results The experimental results showed that PSS could improve spleen cell proliferation,enhance macrophage phagocytic function and inhibit T-cell and B-cell proliferation.Conclusion PSS possessed significant immunoregulation effect,whilst the immunocompetence comparison of PSS' fractions proved that the different immunocytes had different requirements for saccharides length.