Objective:To study the corrosion resistance of four metal-ceramic alloys in the presence of Streptococcus sobrinus, and to compare the effects of bacteria action in liquid and solid environment.Methods:Four metal-ceramic alloys were exposured to BHIA or BHI media with or without Streptococcus sobrinus and served as different groups. After inoculating 10 weeks, their I_ corr ,E_ corr ,E_ tp through cyclic polarization curves were obtained through Corrview 2 corrosion analyses software;and their surface changes after electrochemical corrosion were observed by field emission scanning electron microscopy (FESEM).Results:The E_ corr of four alloys were decreased in presence of oral microorganism, and the declines were more remarkable in BHI with bacteria. SEM observation showed the pittings in their surface.The corrosion resistance in BHI without bacteria were significant increased than that in the blank control. Conclusion:Streptococcus sobrinus can promote the corrosion behavior of metal-ceramic alloys and the corrosion resistance is more worse in BHI than BHIA with bacteria.

The flower-shaped Pt-Au-graphene oxide ( GO ) nanostructures were synthesized by one-step electrochemical reduction method. The electrocatalytic activities of the nanostructures toward glucose oxidation were investigated by cyclic voltammetry. The results indicated that the nanocomposites exhibited a higher electrocatalytic activity than the Pt-Au nanoparticles. A glucose nonenzyme sensor could be easily developed with the use of the flower-shaped Pt-Au-GO nanostructure. The sensor exhibited low detection limit (4. 0 μmol/L), a wide and useful linear range (1. 0-25. 0 mmol/L), and high sensitivity (26. 3 μA cm-2 ( mmol)-1 ) . In addition, the sensor exhibited excellent reproducibility, stability and selectivity for the determination of glucose, and it could be used to analyze real samples.

Objective To assess health-related quality of life and the mood disorder in adults with epilepsy,and to evaluate factors contributing to the quality of life.Methods Quality of life was measured by the Quality of Life in Epilepsy Inventory(QOLIE-31)and the World Health Organization Quality of Life Assessment-Bref(WHOQOL-BREF).Psychotic conditions were evaluated by Self-rating Depressive Scale (SDS)and Self-rating Anxious Seale(SAS).The multivariate analysis was used to assess the determinant factors.Results The study included 141 epilepsy patients in the teat group and 59 sex,age,and education matched normal controls. WHOQOL-BREF scores in the physical and psychological aspects were significantly lower in epilepsy patients(12.7 ±1.8 and 12.4±1.9,respectively)than those in the normal controls(15.1 ±2.3 and 13.9 ±1.9,respectively,t value were 11.75 and 8.625.both P<0.05).The survey reported that 57.4 % of the epilepsy patients suffered with depression.and 39.7 % anxiety.The patients with both anxiety and depression scored lower in all aspects in QOLIE survey except medical effect.Multivariate analysis showed that factors that effect the overall quality of life in order were anxiety.depression and disease duration.Conclusion Our results support that complications of anxiety and depression and long disease duration are key factors affecting the quality of life in epilepsy patients.

Objective: To study the effects of rapid eye movement sleep (REMS) deprivation on active behavior and the expression of mRNA coding for inducible nitric oxide synthase (iNOS) of hypothalamus in rats. Methods: The flower pot technique was adopted to deprive the REMS of Sprague Dawley rats for 24 h, 48 h and 72 h respectively. The active behavior of rats were examined by the open field test (OFT). The expression of mRNA coding for iNOS of hypothalamus in rats was assayed by reverse transcription polymerase chain reaction (RT PCR). Results: The OFT scores were significantly increased in rats subjected to 24 h and 48 h REMS deprivation ( P 0.05). There was no difference in the expression of iNOS mRNA among 24 h, 48 h REMS deprivation and the control groups, but the expression was significantly increased in 72 h REMS deprivation group ( P

Graphene ( GN) and multiwalled carbon nanotubes ( MWCNT) composites were coated on glassy carbon electrode ( GCE ) and then poly ( nicotinic acid ) ( PNA ) was electrodeposited on the modified electrode. The electrochemical behavior of pyridoxine hydrochloride ( VB6 ) was investigated at the modified electrode by cyclic voltammetry ( CV ) and differential pulse voltammetry ( DPV ) . Results showed the oxidation current of VB6 at the GN-MWCNT/PNA/GCE was obviously larger than that at GCE, PNA/GCE and GN/MWCNT/GCE. The oxidation process of VB6 was an irreversible diffusion-controlled process involving one electron and two protons. The liner range between the peak current intensity of DPV and the concentration of VB6 was 0 . 05-200 μmol/L with a detection limit of 0 . 02 μmol/L ( S/N=3 ) . The modified electrode showed a good reproducibility with a relative standard deviation of 3 . 1% ( n=8 ) . The proposed method was applied to the analysis of vitamin B6 in vitamin B6 tablets and compound vitamin B tablets with recoveries between 96 . 1%-104 . 5%.

Objective:To identify the Key genes in the development of sepsis through weighted gene co-expression network analysis (WGCNA).Methods:The gene expression dataset GSE154918 was downloaded from the public database Gene Expression Omnibus (GEO) database, which containes data from 105 microarrays of 40 control cases, 12 cases of asymptomatic infection, 39 cases of sepsis, and 14 cases of follow-up sepsis. The R software was used to screen out differentially expressed genes (DEG) in sepsis, and the distributed access view integrated database (DAVID), search tool for retrieval of interacting neighbouring genes (STRING) and visualization software Cytoscape were used to perform gene function and pathway enrichment analysis, Protein-protein interaction (PPI) network analysis and key gene analysis to screen out the key genes in the development of sepsis.Results:Forty-six candidate genes were obtained by WGCNA and combined with DEG expression analysis, and these 46 genes were analyzed by gene ontology (GO) and Kyoto City Encyclopedia of Genes and Genomes (KEGG) pathway enrichment to obtain gene functions and involved signaling pathways. The PPI network was further constructed using the STRING database, and 5 key genes were selected by the PPI network visualization software Cytoscape, including the mast cell expressed membrane protein 1 gene (MCEMP1), the S100 calcium-binding protein A12 gene (S100A12), the adipokine resistance factor gene (RETN), the c-type lectin structural domain family 4 member gene (CLEC4D), and peroxisome proliferator-activated receptor gene (PPARG), and differential expression analysis of each of these 5 genes showed that the expression levels of the above 5 genes were significantly upregulated in sepsis patients compared with healthy controls.Conclusion:In this study, 5 key genes related to sepsis were screened by constructing WGCNA method, which may be potential candidate targets related to sepsis diagnosis and treatment.

An ultrasensitive electrochemical immunosensor modified with graphene and dienestrol(DE)was developed for the detection of dienestrol through indirect competition with K3Fe(CN)6 acting as the redox probes. The results revealed that under optimized conditions a calibration for DE was obtained with a linear range of 500-5 000 ng/mL and the detection limit was up to 0. 2 ng/mL, showing that the proposed electrochemical immunosensor had excellent sensitivity and wide detection range. The immunosensor was examined in real samples for the analysis of DE. A good recovery in the range of 83. 8%-97. 7% was obtained in pork samples.

Objective To investigate the risk factors of bladder spasm after transurethral resection of the prostate (TURP) and the intervention effect of cluster nursing. Methods From January 2011 to December 2012, the clinical data of 120 cases of TURP patients were analyzed for the risk factors of bladder spasm after TURP. According to the results of the analysis, cluster nursing plan was constructed. The 150 TURP patients from January 2013 to December 2014 were selected as the control group and given routine nursing care. The 150 TURP patients from January 2015 to December 2016 were recruited as the observation group and given cluster nursing. The postoperative bladder spasm, catheter blockage and patients satisfaction were compared between the two groups. Results The results of the multivariate regression analysis indicated that the score of Self-rating Anxiety Scale, air bag water injection, unstable bladder or low compliance bladder, and postoperative constipation were independent factors of bladder spasm after TURP (OR=1.427, 0.092, 13.263, 27.437; P<0.01). After the implementation of cluster nursing, the postoperative bladder spasm rate was 6.0%, and the catheter blockage rate was 5.3%, which were both lower than those of the control group (28.4%,29.3%), and the differences were statistically significant (χ2=25.726, 30.149; P<0.01). The patient postoperative satisfaction of the observation group was (95.21±4.63), which was higher than that of the control group (85.76±4.23), and the difference was statistically significant (t=14.469, P<0.01). Conclusions Cluster nursing can reduce the rate of bladder spasm and catheter blockage after TURP, and increase patients' satisfaction after surgery.

Objective To determine the impact of progressive rehabilitation intervention based on the FITT principle on the recovery of elderly patients with acute ST-segment elevation myocardi-al infarction(STEMI)after percutaneous coronary intervention(PCI).Methods A total of 184 STEMI patients admitted to our hospital between December 2021 and June 2023 were enrolled and randomly divided into a study group(n=92)and a control group(n=92).Routine rehabilita-tion intervention was given to the control group,and progressive rehabilitation intervention based on the FITT principle was given to the study group.Both groups were intervened for 12 weeks.Metabolite equivalents(MET)of the two groups were evaluated before and at 4,8 and 12 weeks after intervention.Their cardiopulmonary reserve function and quality of life were evaluated with cardiopulmonary exercise testing and myocardial infarction multidimensional assessment scale(MIDAS)before and at 12 weeks after intervention.The incidence of adverse cardiovascular events such as ventricular arrhythmia and heart failure(HF)during 6 months of follow-up was recorded.Results There were significant differences in two sets of MET value groups,time points,and group time point interactions(P＜0.01).No statistical difference was found in MET values be-tween the two groups before intervention(P＞0.05).The MET values were increased in both groups at 4,8 and 12 weeks after intervention than before intervention,and those in the study group were higher than those in the control group at above three time points(P＜0.05).Com-pared with before intervention,the peak oxygen uptake and anaerobic threshold oxygen uptake in 2 groups increased after intervention(P＜0.05).The peak oxygen uptake and anaerobic threshold oxygen uptake in the study group were higher than those in the control group[(23.52±1.86)ml/(min·kg)vs(21.36±2.60)ml/(min·kg),P＜0.01;(14.33±2.02)ml/(min·kg)vs(12.08±1.76)ml/(min·kg),P＜0.01];Compared with before intervention,the carbon dioxide ventilation equivalent slope of 2 groups decreased after intervention(P＜0.05).The carbon dioxide equivalent slope of the study group was lower than that of the control group(26.12±2.34 vs 28.10±2.52,P＜0.01).Compared with before intervention,the MIDAS score was decreased in both groups af-ter intervention(65.30±5.20 vs 96.64±7.33,P＜0.01;70.46±5.87 vs 95.80±7.26,P＜0.01),and the score of the study group was lower than that of the control group(65.30±5.20 vs 70.46±5.87,P＜0.01).After a 6-month follow-up,there was no significant difference in the incidence of adverse cardiovascular events between the two groups(P＞0.05).Conclusion Progressive reha-bilitation interventions based on the FITT principle can promote exercise tolerance,improve car-diopulmonary reserve function,and enhance the quality of life of elderly STEMI patients after PCI.

Objective:To investigate the relationship between SNP and noise-induced hearing loss (NIHL) susceptibility in occupational noise exposure population in China.Methods:From 6297 for a certain steel works in contact with noise, contact length of 3 years or more and workplace noise exposure intensity of 80 dB (A) , ears or high frequency (3 000, 4 000, 6 000 Hz) average of hearing acuity 40 dB (HL) , or high frequency loss in both ears, on the basis of single whisper frequency (500, 1, 000, 2 000 Hz) average threshold of 26 dB (HL) or object as case group. A case-control study was designed with 1:1 matching. Subjects with the same gender, the same type of work, age ±5 years old, and working age ±2 years after noise exposure were selected as the control group. Subjects with any whisper frequency (500, 1, 000, 2, 000 Hz) whose hearing threshold in any frequency band was ≤25 dB (A) and whose average high-frequency hearing threshold in pure tone hearing test was <35 dB (A) were selected as the control group. Four sites of PON2 gene were genotyped by medium-and high-throughput SNP genotyping. Univariate logistic regression was used to analyze the relationship between single SNP polymorphism and NIHL.Results:A total of 286 case-control pairs were included. Smoking was statistically significant difference between cases and controls ( P<0.001) . Conclusion:No statistical difference has been found between single SNP polymorphism and NIHL. At the level of greater than 92 dB of high noise exposure, rs7785846 (CT+TT) genotype is a risk factor for occupational noise deafness, and its OR is 2.74 (95% CI: 1.09-6.89) compared with wild homozygous type (CC) . Conclusion. The rs7785846 (CT+TT) genotype carriers of PON2 gene are more susceptible to hearing impairment when exposed to high noise intensity.