Objective To investigate the effect of electro-acupuncture on behavior alterations,the expression of amyloid precursor protein(APP),amyloid β-protein(Aβ) proteins and neuroapoptosis in the cerebral cortex in the APPswe/PS1dE9 double transgenic mice with Alzheimer's disease (AD) induced by isoflurane.Methods Sixmonth-old AD mice and wild-type mice at the same age were randomly divided into wile-type control group,AD group,isoflurane group,electro-acupuncture group (N =8).The mice were given pretreatment with electro-acupuncture at Baihui(GV20) acupoint and Yongquan(KI 1) acupoint once a day for 3 successive days,15 min each time.And then the mice in electro-acupuncture group and isoflurane group were exposed to a box full of 1.2％ isoflurane for 4 hours.Morris water maze was used to test the learning and memory abilities of the mice,Western blotting method was used to detect the expression of APP-C83,APP-C99 and Aβ,and terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick end labeling(TUNEL) was used to detect neuroapoptosis in the cerebral cortex.Results The escape latency of AD group was longer than that of wild-type mice group(P＜0.05),and the latency of isoflurane group was longer than that of AD group (P ＜ 0.05),while the latency of electro-acupuncture group was shorter than that of isoflurane group(P ＜ 0.05).The percentage of retention time in the target quadrant and the times for crossing the target quadrant in isoflurane group were lower than those of AD group (P ＜ 0.05),but were higher in electro-acupuncture group than those in isoflurane group (P ＜ 0.05).APPC83 expression level in isoflurane group was significantly lower than that in AD group (P ＜ 0.05),while APP-C83 expression level in electro-acupuncture group was higher than that in isoflurane group (P ＜ 0.05).APP-C99 expression level in isoflurane group was significantly higher than that in AD group (P ＜ 0.05),and APP-C99 expression level in electro-acupuncture group was lower than that in isoflurane group (P ＜ 0.05).The cortical apoptosis index in isoflurane group was significantly higher than that in AD group (P ＜ 0.05),and the cortical apoptosis index in electro-acupuncture group was lower than that in isoflurane group (P ＜ 0.05).The expression level of Aβ in AD group,isoflurane group and electro-acupuncture group was significantly higher than wild-type control group (P ＜ 0.05).Conclusion Electro-acupuncture can relieve the AD-like neurotoxicity induced by isoflurane and inhibit the decline of learning and memory abilities of AD mice,and the mechanism is probably related with suppressing the overexpression of APP-C99 and reducing the production and accumulation of Aβ,thereby alleviating the neuroapoptosis.

From 2006 to 2008, the susceptibility of different species of animal hosts to Campyebacter jejubni infection was observed in various areas of Jiangsu province, in which the API Campy System was used to perform the biochemical identification and the multiple PCR assay was employed to analyse the C.jejuni isolates from 3010 specimens of fouls. Cattle, pigs and monkeys, and in addition, the susceptibility of isolates to various antibiotics was also determined. In these specimens investigated 402 samples were found to be positive in the detection of C.jejuni with a positive detection rate of 13.36%. The positive detection rates in chicken, water fouls, milk cows, pigs, monkey, red crowned crane and wapiti were 15.83% (258/1630), 10.4% (52/100), 8.24% (42/510), 15.63% (25/160), 15% (15/100), 12.5% (15/80) and 0% (0/30) respectively. Meanwhile, the antibiotics to which the isolates from different hosts showed high rate of sensitivity to 27 antibiotics of 10 varieties included: chloromycetin (100%), Almocylin (99.7%), amicarcin (92.59%), cefprozil (91.67%), alchimycin (90.74%); while the antibiotics to which these isolates showed high rate of resistance were compound neoromin (99.7%), cefoperazone (99.07%), trimethoprim (97.22%), cepronatin (91.67%), cepromondo (99,07%) respectively. It is evident that the susceptibility of different hosts to C.jejuni infection and the status of drug-resistance of the isolates appear to be quite different and more complicated in Jiangsu province.